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Characterization of Selenium-Modulated Effects and Kinetics Research on Rat Dorsal Root Ganglion Neurons

Author: YuanHuiJun
Tutor: LinJiaRui
School: Huazhong University of Science and Technology
Course: Biomedical Engineering
Keywords: Nano-selenium (Nano-Se) Sodium selenite Na2SeO3 Patch clamp Whole-cell Rat dorsal root ganglion (DRG) Voltage -dependent sodium current Tetrodotoxin -sensitive sodium currents (TTX-S) Non tetrodotoxin -sensitive sodium currents (TTX-R)
CLC: R341
Type: Master's thesis
Year: 2006
Downloads: 59
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Abstract

Selenium in cancer chemoprevention and treatment has a clear role. However, current research data show that levels of nutrition and its ultra-high dose only when significant effect, these doses close to toxic levels. Nano-Se, using the latest nanotechnology nanoparticles made of its high activity and low toxicity characteristics can release more energy selenium for cancer prevention [13]. Selenium functional studies have been reported on many. However, the role of selenium on the nervous system regulating the research, we also poorly understood. Within the scope of its low toxicity anti-cancer cancer at the same time, whether they would affect the human nervous system? Article using patch-clamp technique, for the first time in SD rat dorsal root ganglion (DRG) for the study, the whole-cell mode under different concentrations of sodium selenite (Na2SeO3) and nano-selenium (Nano-Se) and its regulatory role in the membrane current channel dynamics for a more in-depth study. The results show: Selenium had no effect on cell membrane potassium currents, however, its effect on sodium currents significantly. Regulatory role in a time-dependent and dose-dependent manner. In its nutritional range, voltage-dependent sodium current increases; weak toxicity and toxicity in the range of sodium current decreases. Close and, on the voltage-dependent sodium current analysis found that selenium on TTX-R sodium currents in type no effect, while the TTX-S sodium current model has significantly enhanced and inhibition. In this context, further more detailed studies within the scope of the low toxicity of selenium on voltage-gated sodium channel type TTX-S current regulation and related dynamics analysis. Flip through the membrane potential, membrane conductance, steady-state inactivation, activation and recovery curve and its associated parameters kinetic analysis, the following conclusions: the role of selenium in the channel pore, rather than acting on the external surface of the channel protein channel protein or near external surface. Channel blocking effect of selenium is - open block, i.e. when the channel opening has a blocking current. Finally, in the nano-selenium and sodium selenite comparative experiments analyzing the results, we propose the following conclusions and reasonable assumptions: 1) maintaining efficient biological activity, while nano-selenium selenite lower than the short-term toxicity, This ensures that this new type of nano-drugs in anti-cancer therapy has been widely used; 2) the role of nano-selenium and sodium selenite competition sites on the same channel, first with the binding site of the drug makes the follow-up Drug due binding site can not be invalidated; 3) nano-selenium and sodium selenite different competitive within the channel loci, the first position and the corresponding point of the drug combination of drugs can added binding site mutation resulting in subsequent failure of drug action.

Characterization of Selenium-Modulated Effects and Kinetics Research on Rat Dorsal Root Ganglion Neurons

Abstract

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