Dissertation > Excellent graduate degree dissertation topics show

The Role of Redox Factor 1 in Ischemia Preconditioning for Reduced-for-size Liver Transplantation of Rats

Author: LiuXianZhong
Tutor: LiXiangCheng
School: Nanjing Medical University
Course: Surgery
Keywords: Rats Liver transplantation Reduced-size liver transplantation Ischemic preconditioning Ischemia-reperfusion injury Redox factor 1
CLC: R657.3
Type: Master's thesis
Year: 2007
Downloads: 47
Quote: 0
Read: Download Dissertation

Abstract


The purpose of establishing the Department of reduced-size liver transplant case studies with similar clinical reduced-size liver transplantation animal model. Past established a small animal model of reduced-size liver transplant research. The establishment of a small animal model of liver transplantation is the basis of the experimental study of liver transplantation, liver transplantation animal model international rat orthotopic liver transplantation model, such as the classic Kamada \reduced-size liver transplantation in rat liver ischemia-reperfusion injury and ischemic preconditioning mechanisms in-depth study, the research group by reference Xiang-Cheng Li, using a modified \reduced-size liver transplantation in rats (reduced-for-size liver transplantation, RSLT) model. Objective To observe the ischemic preconditioning (ischemia precondition, IPC) reduced-size liver transplantation redox factor -1 (Redox factor-1, Ref-1) protein expression to explore IPC reduced-size liver transplantation injury in rats the protective effect and its mechanism. 100 Lewis adult male rats were randomly divided into three groups: IPC transplantation group (IPC group), 50% of the transplant group (PLT group) and sham-operated group (SO), respectively, after transplantation, 0.5h, 2h, 6h 24h sacrificed at various time points by Western immunoblotting hybridization and immunohistochemical techniques combined with image analysis quantitative detection transplant Ref-1 expression changes, combined with serological and histopathological analysis of the significance of Ref-1 expression. The results showed that serum ALT values ??were postoperative PLT group at each time point (595.06 ± 108.78) U / L,; (723.18 ± 117.24) U / L; (1186.65 ± 142.31) U / L; (1498.91 ± 126.79) U / L; serum ALT values ??after IPC group at each time point were (459.06 ± 84.73) U / L,; (587.71 ± 95.23) U / L,; (799.61 ± 125.97) U / L,; (659.27 ± 135.68) U / L,. IPC group after 6h and 24h serum ALT values ??decreased significantly compared to the PLT group (t = 4.553, P <0.05; t = 10.110, P <0.01). The pathology analysis shows the The PLT group after 24h periportal inflammatory cells infiltrating the liver sinusoidal dilatation significantly heavier liver injury and can be seen; IPC group less damage. Compared to the PLT group, IPC group, reduced-size liver transplantation 24h observed in Ref-1 protein expression was significantly higher (P <0.05) in the liver parenchymal cells. Conclusion Ischemic preconditioning can reduce the reduced-size liver transplantation early liver damage, and promote liver tissue Ref-1 protein expression, suggesting that ischemic preconditioning less size liver graft early injury mechanisms may promote Ref-1 protein expression .

Related Dissertations

  1. Effects of CrPyr on Protein Metabolism in Rats,S865.12
  2. The Protective Effect of Rat Myocardial Ischemia/reperfusion Injury Following Bone Marrow Mesenchymal Stem Cell Pretransplantation for 1 Week,R542.22
  3. The Effects of Atorvastatin on Expression of Osteopontin in Kidney of the Diabetic Rats Its Molecular Mechanism,R587.2
  4. Meridians of Asthma Thread Burying Model Rats IL-4 Such Impact Experimental Study,R245
  5. The Study of the Effects of Isoflavones of Puerarin and Daidzein on Bone Metabolism in Ovariectomized Female Rats,R580
  6. Effect of Microwave on GAP-43 Expression in the Spinal Cord after Sciatic Nerve Lesions in Rats,R651.3
  7. The Expression and Mechanism of NF-κ B and IL-6 after 90% Portal Vein Ligation in Rats,R657.3
  8. In Vitro Culture and Identification of Vascular Smooth Muscle Cells from Rat Intra- and Extracranial Arteries,R743.3
  9. Effects of Different Species Meats on Growth, Digestion Metabolism and Antioxidant Status in Rats,S865.12
  10. Effect of Grape Seed Procyanidins on the Blood Pressure in Renovascular Hypertensive Rats and Its Mechanism,R544.1
  11. The Research on Treatment of Lentivirus-mediated hVEGF-165 Gene Transferring Mesenchymal Stem Cells on Rat Hind Limb Ischemia,R329
  12. Experimental Study of Neural Stem Cells around the Lateral Ventricular Zone of the Hydrocephalus Rats,R742.7
  13. Effect of Nrf2-ARE Pathway on Isolated Rat Hearts Using Ischemia or Pinacidil Postconditioning,R96
  14. Anti-gout Capsules on the Treatment of Gouty Arthritis and Its Mechanism,R259
  15. Experimental Study of GUI QIN MISTURE on Adriamycin-induced Nephropathy in Rats,R285.5
  16. Effects of Losartan on Na~+, K~+-ATPase in Proximal Tubules Epithelial Cells of Spontaneously Hypertensive Rats,R544.1
  17. The Influence of Cold Self-bloodcardioplegia to MMP-2 Concentration for Infant with Cardiopulmulnary Bypass,R726.5
  18. Morphological Characteristics of Astrocytes in EAN–a Tricellular System for Study on Neuroprotection and Neuroregeneration,R651.1
  19. The Relevance Research of Neuroglobin and Caspase-3 in the Hippocampus CA1 Regin in Global Cerebral Ischemia-reperfused Rats,R741
  20. Neuroprotective Mechanism of NBP Preconditioning in Cerebral Ischemia Reperfusion Injury on Sprague-Dawley Rats,R743.33
  21. Exogenous PCr anti- animal brain ischemia-reperfusion injury and brain-derived experimental study MODS,R965

CLC: > Medicine, health > Surgery > Of surgery > Abdominal surgery > Liver and liver tube
© 2012 www.DissertationTopic.Net  Mobile