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The Study on the Expression of Apoptosis in the Mouse Myocardium/Brain after Ketamine Administration

Author: YangJu
Tutor: BianShiZhong
School: Suzhou University
Course: Forensic
Keywords: Ketamine Drug Abuse Myocardial cells Brain cells Apoptosis Caspase-3 TUNEL
CLC: D919
Type: Master's thesis
Year: 2007
Downloads: 179
Quote: 0
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Abstract


Objective: To observe ketamine chronic poisoning induced expression of mouse cardiac / brain cell apoptosis. Methods: Using the mouse tail vein administration, the establishment of ketamine poisoning in mice model. 90 Kunming mice were randomly divided into control and experimental group and 10 control group, the experimental group (n = 80), the group is divided into 1, 2, 4, 8, 12 weeks in the control group and the experimental group, the experimental group mice at each time point were given 4mg/kg, 10mg/kg, 20mg/kg, 30mg/kg ketamine injection, the control group were given saline. Observe and record the behavior of ketamine poisoning mice histological changes; observed by light microscopy ketamine chronic poisoning histopathological changes in mice; ketamine poisoning caused by myocardial cells was observed by transmission electron microscopy and ultrastructure of nerve cells apoptosis-related changes, qualitative detection; deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) staining observed DNA nucleosome degradation, count the number of apoptosis cells, quantitative detection; in order to infer the pathway of apoptosis, confocal microscopy activated Caspase-3 protein expression. Results: (1) ketamine poisoning mice mouth and facial twitching and nodding, forelimb clonus behavior changes, the changes related to the dose of ketamine, with the increasing doses of ketamine, mice behavioral changes more obvious. (2) the HE staining observed by light microscopy results show that the control group no significant abnormalities, normal mouse tissue cell structure of the experimental group, deeply stained nuclei or nuclear-free change. (3) transmission electron microscope (TEM) results showed that the mice of each experimental group were seen in ketamine poisoning one week of myocardial cells / brain tissue nuclear condensation, margination, fragmentation, Ketamine can induce apoptosis of myocardial cells and brain tissue. (4) TUNEL results showed that ketamine poisoning one week myocardial tissue positive cells increased significantly compared with the control group, a significant difference (P lt; 0.05); thereafter gradually reduced with the time of apoptotic cells was time-dependent which to 20mg/kg experimental group at 12 weeks, TUNEL-positive cell count is still higher than that of the control group (P lt; 0.05), of 30mg/kg experimental group of TUNEL-positive cell counts in the first eight weeks when compared with the control group significant difference (P gt; 0.05). Ketamine poisoning, 4 to 10mg/kg experimental myocardial apoptosis in a dose-dependent increase in the dose-effect relationship is not obvious in the 20 30mg/kg experimental group. TUNEL assay showed that ketamine poisoning one week, the brain tissue positive cells increased significantly, compared with the control group, there were significant differences (P lt; 0.05); followed over time, gradually reduced the number of apoptotic cells was time-dependent, which 4 to 20mg/kg experimental group at 12 weeks, TUNEL-positive cell counts with the control group still significant differences (P lt; 0.05), of 30mg/kg experimental group of TUNEL-positive cell counts in the first eight weeks with the control group no significant difference (P gt; 0.05) Ketamine poisoning at different times, a dose-dependent increase in cell number of apoptotic cells of the brain tissue of 4 to 10mg/kg experimental group, dose-effect relationship is not obvious in the 20 to 30mg/kg experimental group. (5) confocal microscopy results showed that ketamine poisoning one week increase in myocardial tissue of mice Caspase-3 activation number of positive cells compared with the control group were significantly different (P lt; 0.05), the peak in the ketamine poisoning one week, with the with the extension of time Caspase-3 activation number of positive cells in a time-dependent decrease, of which 4 to of 20mg/kg experimental group at 12 weeks, caspase-3 activation positive cells is still higher than that of the control group (P lt; 0.05) 30mg/kg experimental group at 8 weeks when compared with the control group, no significant difference (P gt; 0.05). Ketamine poisoning at different times, to 10mg/kg experimental group myocardial tissue Caspase-3 activation positive cells in a dose-dependent increase in 20 to 30mg/kg dose-effect relationship is not obvious. The confocal results show that ketamine poisoning one week, the number of positive cells, brain tissue of mice Caspase-3 activation was significantly increased and reached a peak, compared with the control group were significantly different (P lt; 0.05), with the time Caspase- 3 activate positive cells in a time-dependent decrease, of which 4 to of 20mg/kg experimental group at 12 weeks, caspase-3 activation positive cells is still higher than that of the control group (P lt; 0.05), 30mg/kg experimental group in 8 weeks, no significant difference compared with the control group (P gt; 0.05) To 10mg/kg experimental brain tissue Caspase-3 activation positive cells in a dose-dependent increase in 20 to 30mg/kg dose-effect relationship is not obvious. Tip Caspase-3 activation positive cell count changes in myocardial and cerebral number of apoptosis same trend. Conclusion: Ketamine and chronic poisoning myocardial tissue cells and brain cells can induce apoptosis mechanism and Caspase-3 activation.

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