Dissertation > Excellent graduate degree dissertation topics show

Cloning and Function Analysis of ZmGW2, A RING-domain Protein

Author: JiangYing
Tutor: LuYunCai；LiHuiYong
School: Heilongjiang University
Course: Crop Genetics and Breeding
Keywords: Zea mays L. ZmGW2 PCR Arabidopsis thaliana Transformation
CLC: S513
Type: Master's thesis
Year: 2011
Downloads: 41
Quote: 0
Read: Download Dissertation

Abstract


High yield play important roles in breeding. In this study, a gene was cloned from maize (Zea mays L.) by RT-PCR method and named as ZmGW2. The initial structure and function of ZmGW2 was analyzed by bioinformatics.The expression of ZmGW2 was detected by sqRT-PCR in different tissues and different treatments. ZmGW2 was introduced into maize and Arabidopsis thaliana by Agrobacterium-mediated transformation.1. Bioinformatics analysis revealed that the predicted ZmGW2 protein has 431 amino acids, and has RING-type domain. all RING proteins act as E3 ubiquitin protein ligases, with implications for a variety of biological areas.2. SqRT-PCR analysis showed that the ZmGW2 expression was induced by salt (NaCl) and abscisic acid (ABA), and ZmGW2 was resistant to drought and cold. Furthermore, the ZmGW2 showed different expression patterns in different tissues and was more abundant in stems and kernels. ZmGW2 expression was higher in silk of F1 than parents. These results suggest that may play multiple roles in yield, plant stress response, as well as reproductive growth and development.3. Over-expression vector of ZmGW2 and RNA interference expression vector of AtGW2 were constructed and transformed into Arabidopsis thaliana.The grain size and kilo kernels weight of ZmGW2 overexpression transgenic plants were lower than those of AtGW2 RNA interference transgenic plants. These results suggested that ZmGW2 and AtGW2 negatively regulates the grain size and weight in Arabidopsis.4. Compared with wild-type Arabidopsis, T1 seeds and plants of ZmGW2 overexpression transgenic plants were treated with drought, cold, NaCl and ABA. The growth of transgenic Arabidopsis was all better than wild-type plants. The results showed that ZmGW2 may play multiple roles in plant stress response.

Related Dissertations

  1. Rebellion of an and Shi and Poetic Style Evolution from Kaiyuan to Tianbao,I207.22
  2. Research of Wavefront Transformation Based on Liquid Crystal Spatial Light Modulator,TN761
  3. Service Model Driven Architecture (SMDA) Oriented Modeling Techniques and Tool Implemention,TP311.52
  4. Chinese television media discourse paradigm of the evolution of the multi-angle analysis,G220
  5. Cloning and Expression of CHS and CHI Genes and Their Regulation on the Accumulation of Flavonoids in ’Cara Cara’ Navel Orange (Citrus Sinensis Osbeck) and ’Guoqing NO.4’ Satsuma Mandarin (Citrus Unshiu Marcow),S666.4
  6. Studies on the Quality of Phisio-Chemical and Fresh Keeping Technologies of Post-Harvest in Volvariella Volvacea,S646.13
  7. Construction of the Recombinant of HSV-2gD Minotope、 HBsAg、 IL-18 DNA Vaccines and Its Inducing Immune Respons in Mice,R392
  8. Rural Energy Transformation and the Benefits of Ecological Environmental,S210.3
  9. Directed Evolution of Lipase from Penicillium Expansum FS1884 by Error Prone PCR,Q78
  10. Improvement of the Resistance of Nicotiana Tabaccum to Virus by RNAi and Initial Establishment of Genetic Transformation System of L.regale,S435.72
  11. Xu Peng age of academic thought and clinical experience medication regularity of the treatment of chronic gastritis,R249.2
  12. Based on statistics of the lognormal distribution heteroscedasticity model inferred,O212.1
  13. Mutagenesis of a Laccase Gene from Pleurotus eryngii in Vitro and Expression in Pichia Pastoris,TQ925
  14. Survey in Nanjing and Analysis of the Mitochondrial Genome of Frankliniella Occidentalis (Pergande),S433
  15. Identification of Cyto-and Molecular Genetics of Multisomic Addition Line "Nj08-063" in Brassica Napus and Its Agronomical Traits,S565.4
  16. Transformation of RdreBlBI Gene Drived by Rd29A in ’Benihope’ Strawberry,S668.4
  17. GIH cDNA Cloning and Mrna Expression in Litopenaeus Vannamei,S917.4
  18. The Detection of the Virulence-related Gene from Vibrio Alginolyticus and the Study on Preservation Methods,S943
  19. Gene Prediction and Sequence Analysis of Insect OBP CSP and sid-1,Q78
  20. Cloning and Expression Analysis of Scavenger Receptor Class B Type Ⅰ and Antifreeze Proteinstype Ⅱ Genes in Lutjanus Sanguineus,S917.4
  21. Isolation, Identification and Molecular Characteristics of Japane Encephalitis Virus in Henan Province,S852.65

CLC: > Agricultural Sciences > Crop > Cereal crops > Corn ( maize )
© 2012 www.DissertationTopic.Net  Mobile