Dissertation > Excellent graduate degree dissertation topics show

Purification, Structural Characterization and Biological Activity of Peptides from the Skin of Rana Nigromaculata

Author: WangJian
Tutor: ShangDeJing
School: Liaoning Normal University
Course: Genetics
Keywords: Skin Bioactive Polypeptides Structure Identification Mass Spectrometry Detection
CLC: Q51
Type: Master's thesis
Year: 2011
Downloads: 112
Quote: 0
Read: Download Dissertation

Abstract


Amphibians is the most primitive terrestrial vertebrates adapt to both terrestrial and aquatic life. Amphibians skin is uncovered and without hair, feathers and shells. Amphibians skin play an important role in the complex and diverse environment to maintain its own living. Amphibians skin contains biological vivid substances with various comploicated biofunctions, and these substances play an important role in resisting the invasion of harmful environmental factors. Meanwhile, the skin of different Amphibians species consists of different bioactive substances. Amphibian’s skin is rich in natural resources and used in new bioactive peptides’discovery, refiner and function research. Rana nigromaculata of China is one of animal resource, which has medical value in northeast regionof china. Researches have shown that Rana nigromaculata skin contains various active peptides, such as bradykinin-like peptide, bombesin peptide, growth factor, morphine and moisturizing factors.In this paper, Rana nigromaculata growth in Jinzhou Dengshahe of Dalian of Liaoning was choosed as the experimental material. The isolation, purification and structural characterization of bioactive peptides from the skin of Rana nigromaculata were carried out by gel chromatography and RP-HPLC. The optimized conditions of purification were screened, and seven of skin bioactive peptides were obtained. MS and Q-TOF MS/MS were used to determinate their molecular weight and amino acid sequences. Rana nigromaculata skin secretion was obtained by electrical stimulation. The secretion was purified using VydacTP510 RP-HPLC into 6 single peptides.The results of analysis of amino acid sequences showed that three of these peptides were bradykinin or bradykinin-related peptides, and the other three were antimicrobial peptides. Their molecular weights were from 1060.2 to 3285.96 Dalton, and composed of 9 to 32 amino acids. Their primary structure was RPPGFSPFR,,RPPGFSDSSPLAPGT,LKNVGKEVGFDVENELRLAGHARPLGK,KSPGV GALAGKKPHGLLLSGLKNVGGAEAGFD,LGLGKVLGVGQRALAHSPPK and LVPLV SYHLPVL. After BLAST searched in NCBI Protein Databases, we found that peptides RPPGFSPFR was typical Bradykinin composed of 9 amino acids and RPPGFSDSSPLAPGT had the motif of Bradykinin (RPPGFS-) and belonged to bradykinin-related peptides,named it Ranakinin-RN1.Although the peptide LVPLVSYHLPVL does not contain the conserved domain of bradykinin, we still named it as Ranakinin-RN2 due to its higher homology bradykinin activity. Meanwhile, the rest of peptides shared a lower homoloy with some known amphibian skin peptides. Therefore, they are the new peptides, named them as nigromin-1,nigromin-2, nigromin-3, respectively.The bradykinin activity of bradykinin and bradykinin-related peptides was tested by assaying the contractile activity on isolated guinea pig ileum. The results showed Bradykinin-RN and Ranakinin-RN1 contracted guinea pig ileum in a positive linear relationship, bradykinin activity of Bradykinin-RN was more potent than Ranakinin-RN1. bradykinin activity of Ranakinin-RN2 was inhibited after reaching its maximum, showing that the peptide might be the bradykinin antagonist peptide.

Related Dissertations

  1. The Application of Five Falp Z-plasty with the Fasciocutaneous Scar Skin Flap in Reconstructing Webbed Cicatricial Contracture Deformity of Axilla,R622
  2. Study on Antioxidant Components of Tibetan Medicine Sanguo Tang San,R29
  3. The Influence of Long-term Regular Exercise to the Distribution and Change Regulation on Human Skin Temperature,G804.2
  4. Studies on the Extraction, Saparation and Purification of Red Pigment and Procyanidin of Red Skin of Peanuts,TS202.3
  5. Radiation therapy of acute radiation-induced skin reactions Prevention Research Status,R473.73
  6. The Research of Histopathologic and Related Factors in Chronic Dacryocystitis,R777
  7. Effects of resveratrol on the experimental study of the natural aging mice GSH and superoxide anion radical content change,R285.5
  8. Study on Preparation and Characterization of Collagen from Carp Scales,TS254.9
  9. Cause Analysis and Solution of Tube Burst Caused by Oxide Skin Peeling on a Supercritical Boiler,TK228
  10. Research on the Technologies of Special Content-Based Erotic Image Filtering,TP391.41
  11. Study on Extraction, Purification and Structure Identification of Anthocyanins from Sunflower Seed Hull,TQ464.3
  12. Theoretical and Experimental Study of Helium Leakage Mechanism of Aerostat Envelope Materials,V250.2
  13. Studies on Charge Storage Stability of Electret Transdermal Patches and Mechanism of Drug Transdermal Transfer,R94
  14. The Advantages of a Novel CoQ10 Delivering System in Skin Aging Protection,R758.1
  15. Research on Facial Feature Extraction Algorithm Based on Gabor Filter,TP391.41
  16. Research and Implementation of Network Sensitive Information Filtering,TP393.09
  17. The Clinical Study on Efficacy about Wu Gong Bai Du Yin Decoction in the Treatment of Psoriasis Valgaris (The Type of Yuzhijifu),R275.9
  18. Application of Soft Tissue Expansion in Craniofacial Plastic and Reconstructive Surgery,R622
  19. Survey of Inhaled Allergens of Allergic Rhinitis in Yinchuan,R765.21
  20. Evaluate the Efficacy and Safety of Variable Pulse Width Frequency Doubled Nd: YAG Laser in the Treatment of Dermovascular Disease,R758.6
  21. Studies on Preparation and Properties of Ascorbic Acid Nanoliposomes,TQ466.3

CLC: > Biological Sciences > Biochemistry > Protein
© 2012 www.DissertationTopic.Net  Mobile