Dissertation > Excellent graduate degree dissertation topics show

Phenotype Analysis of Arabidopsis Thaliana AtBAG T-DNA Mutants and Transformation of Arabidopsis by Agrobacteria Harboring AtBAG Overexpression Vectors

Author: DengLi
Tutor: YangWanNian
School: Central China Normal University
Course: Botany
Keywords: Arabidopsis thalina AtBAG NaCl stress Green fluorescent protein
CLC: Q943.2
Type: Master's thesis
Year: 2008
Downloads: 278
Quote: 2
Read: Download Dissertation

Abstract


The co-chaperone Bcl-2 associated gene product（BAG）proteins was first identified in animals.Analysis of the Arabidopsis thaliana genome revealed eight genes encoding proteins with a conserved BAG domain.The BAG-like protein family in A.thaliana can be grouped into three classes based on the presence of other conserved domains.ClassⅠconsists of AtBAG-1,AtBAG-2,AtBAG-3,and AtBAG-4,each containing an additional ubiquitin-like domain.These four proteins are structurally similar to the human BAG-1 proteins and might be BAG-1 orthologs in plants.ClassⅡconsists of only one protein AtBAG-5 that has no other conserved motifs.ClassⅢincludes AtBAG-6,AtBAG-7, and AtBAG-8,which may be regulated by Ca2+because of the presence of a calmodulin-binding domain.In order to study the function of AtBAG,We identify two BAG genes:AtBAG4 and AtBAG7,and analysis the phenotype of the Arabidopsis thaliana AtBAG T-DNA mutants.We find that these mutants have no obvious differences against wild type,they can growing,flowering and seeding.Treat Arabidopsis with oxidative,salt stress between wild-type and the homozygous AtBAG T-DNA mutants,their tolerance to stress were compared.It was inferred that AtBAG maybe have some effect on Arabidosis resistance to PCD.To confirm the function of BAG protein in NaCl stress in Arabidopsis thaliana,we constructed new plant expression vector contain BAG gene and GFP: pBI121-GFP-AtBAG7 and pBI121-GFP-AtBAG4.The recombined plant expression vector were transferred into Agrobacterium tumefaciens strains of GV3101 and transformed Columbia wild-type Arabidopsis with immature flower cluster,and obtained the Arabidopsis thalina AtBAG over expressing mutants of T1.It is a good start to study the influence of over expressing in the phenotype of the Arabidopsis thaliana,and the distribution of BAG protein in the cell ofArabidopsis thaliana.

Related Dissertations

  1. Effects of Exogenous GA3 and 6-BA on Physiological and Biochemical Characters of Different Strawberry Varieties’ under NaCl Stress,S668.4
  2. The Studies of Adhesion of Vibrio Fluvialis to Epinephelus Awoara,S943
  3. The Effects of NaCl Stress on Seed Germination and Physiological Indexes of Seedlings of Two Species of Halophytes,Q945.78
  4. Sequence Analysis of Internal Transcribed Spacers (ITS) of Populus Wutunensis and Physiological Responses to Salt Stress,S792.11
  5. Study on Salt Tolerance of Four Different Rootstock-scion Apple Combinations,S661.1
  6. Effects of Lead and NaCl Stress on Physiolagical Respense of Hydrangea Macrophylla,S682.19
  7. Physiological Mechanisms of Salt Tolerance Improved by Gratfing in Mini-Watermelon Seedlings,S651
  8. Construction and Identification of Liver-specific Vector,R346
  9. Effection of Exogenous ALA on Pumpkin under NaCl Stress,S642.6
  10. Study on Physiological and Biochemical Changes of Alfalfa under NaCl Stress,S541.9
  11. Effect of Different Nitrogen Level on the Growth of Kosteletzkya Virginica Under Nacl Stress,Q945.78
  12. Study on Tolerant Ability of Three Species of Photinia L. under NaCl Stress,S687.2
  13. Study on Effects and Assessments of NaCl Stress on Seed Germination and Physiological and Biochemical of Seedling of Herbages,S54
  14. Studied on Propagation and Salt Resistance of Dichroa Febrifuga L.,S567.19
  15. The Study on Transferring of Human Tumor Suppressor Gene P53 in Chicken,S831
  16. Modified Responses to Salt Stress of Catharanthus Roseus by Exogenously Supplied Nitrogen Forms,S567.239
  17. hTERT promoter PE38KDEL gene vector and its expression in MiaPaCa2 cells,R735.9
  18. Identification of Genes Related to Virulence of Fusarium Graminearum and Biopaning of DON Toxin Specific Affinity Peptides,S432.4
  19. Occurence and Transmission of Airborne Escherichia Coli in Chicken House,S858.31
  20. Construction of the CRE/loxP Recombination Activation System for Analysising the Function of Stress Tolerance Genes and Promoters,S336
  21. Detection of Anti-TMV Activity of Brucein D by Green Fluorescent Protein Labeling,S435.72

CLC: > Biological Sciences > Botany > Plant Cell Genetics > Plant Genetic Engineering
© 2012 www.DissertationTopic.Net  Mobile