|
Gray leaf spot disease caused by Cercospora spp corn (Gercosporazeae-maydisTehon Daniels) disease in the world. Since the 1990s, promote the cultivation of the susceptible varieties, the disease is increasing year by year, has become one of the important diseases of the North East of spring maize area. Practice has proved that the cultivation and planting resistant varieties is the most effective way of prevention and treatment of gray leaf spot, which depends on the results of breeding for disease resistance against the source of the screening and identification of disease inheritance of understanding. In this paper, from the anti-corn gray leaf spot germplasm screening, using backcross introgression lines to explore and verify the resistance gene locus, Group mixed packet analysis to find the gray leaf spot resistance molecular markers, three gray leaf spot disease preliminary study. The main results are as follows: (1) seriously ill prone areas of gray leaf spot by three in the year, 66 of China's major maize inbred lines of gray leaf spot resistance identification, screening out high resistance, disease resistance, anti-susceptible , sense of material 2,15,13,7,29 of copies, respectively, accounting for 3.1% of the total number of test materials, 22.7%, 19.7%, 10.6% and 43.9%. Combined analysis of an inbred germplasm groups, to only PB groups germplasm performance for high resistance or resistance to disease, Sipingtou Ludahonggu, PA, BSSS groups germplasm susceptible or highly susceptible. (2) through (BC4F4) artificial inoculation of Gray Leaf Spot (tuck 478 SH15) x Ye478 Introgression groups identified six resistant plants to verify the corn leaf powder calcium carbonate agar medium gray leaf spot pathogen and artificial spore suspension for injection inoculation method operability; genotypes of SSR markers resistant plants, through linkage disequilibrium analysis in section 3 (3.08) 5 (5.03), 8 (8.05-8.06) on chromosome The detected three sections with gray leaf spot disease resistance gene action additive effect. (3) Group mixed packet analysis, 10 resistant and 10 susceptible maize inbred lines were constructed resistant pool and susceptible pool. With AFLP marker analysis of resistant and susceptible pool, screened 54 polymorphic primers from 100 AFLP primer; further verify the formation of resistant and susceptible pool 20 inbred lines detected eight polymorphic markers; fragment, cloned and sequenced the AFLP markers (P51M38-200) into SCAR markers (Scar-100). Use Scar-100 mark of 66 inbred lines the marker genotypes analysis, combined with field resistance phenotype correlation analysis, prove the Scar-100 mark with gray leaf spot resistance highly correlated; tuck (tuck 478 SH15) × 478 backcross population (BC4F4) 6 marker genotypes resistant plants, their genotype and disease phenotype. Use Mo17 × Huangzaosi, segregating population (190 F2 plants) and X178 × B73 recombinant inbred line population (of 181 F8 pedigree), combined with the existing SSR Markers, Scar-100 Markers in section 3 (3.08 ) chromosome is located SSR the mark umc1399-bnlg1754 and the umc1320-bnlg1754. Scar-100 mark may be a larger application prospects in the resistance to Gray Leaf Spot disease molecular marker-assisted breeding.
|