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Basic Research and Clinical Trial of Tacrolimus Ointment for Treatment of Actinic Injury in Adults

Author: FuLan
Tutor: HeLi
School: Kunming Medical College
Course: Dermatology and Venereology
Keywords: Tacrolimus ( TM ) Ultraviolet A ( UVA ) HaCaT cells Polymorphous light eruption ( PLE ) Chronic actinic dermatitis (CAD)
CLC: R758.1
Type: Master's thesis
Year: 2008
Downloads: 128
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Objective: To explore the proliferation of HaCaT cells after UVA irradiation, tacrolimus and Research Department (TM) HaCaT cells after UVA irradiation expression of ICAM-1 the tacrolimus (TM), the impact of VEGF, clear tacrolimus The mechanism of action of the light-induced skin damage; while observing the 0.1% tacrolimus ointment treatment efficacy and safety of adult photosensitive skin (PLE CAD). Methods: MTT assay different intensity UVA irradiation of HaCaT cells cell activity and cell proliferation TM change; the ELASI and immunohistochemical assay of different intensity UVA irradiation Hacat cells, expression of ICAM-1 and VEGF changes. Selected 30 patients with photosensitive skin oral TRIPTERYGIUM Begonia, the side of the lesions treated with tacrolimus ointment (test side), the other side coated with the compound zinc oxide paste treatment (control side) before treatment and 1, 2 and 4 weeks after treatment, follow-up, the efficacy and safety evaluation. Results: (1) HaCaT cells by UVA irradiation 24h, compared with without UVA irradiation group, cell proliferation was inhibited (P <0.05), TM no significant change (P> 0.05); HaCaT cells by UVA irradiation after 48 hours, with no compared to UVA irradiation, the cells proliferated significantly (P <0.05), TM proliferation was suppressed (P <0.05). 2 HaCaT cells by UVA irradiation after 24 h, compared with cells without UVA irradiation, ICAM-1 expression was significantly increased (P <0.01) compared to TM group ICAM-1 expression was significantly inhibited (P <0.01). 3 HaCaT cells by UVA irradiation after 24 hours than without UVA irradiation cells compared VEGF expression were significantly increased (P <0.05), give the TM group, the expression of VEGF were significantly suppressed (P <0.05) of VEGF expression in the TM concentration of 50 pg / ml when the strongest suppressing effect. ④ the end of treatment, 0.1% tacrolimus the Secretary ointment side effective rate was 93.3% (28/30), with the control efficiency compared to 86.6% (26/30), the difference was statistically significant (P <0.001); skin physiological functions in the detection of patients before and after treatment, the test side and the control side of the skin moisture, grease improvement, but the test side than the control side (P <0.05), the elastic did not change significantly (P> 0.05); self-evaluation in patients with medication before and after the effect, the test side was significantly better than the control side (P <0.001). Test side performance of major adverse reaction to medication site itching / increased itching, burning, but were an over. Conclusion: the first time that (1) TM can inhibit the UVA irradiation of HaCaT cells caused by excessive proliferation, expression of ICAM-1 and VEGF inhibition, revealing TM HaCaT cells after UV irradiation has a protective effect on the the HaCaT cell after UVA irradiation, and UVA-induced inflammation inhibition. (2) 0.1% tacrolimus ointment for the treatment of adult photosensitive skin with a clear efficacy and safety.

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