Dissertation > Excellent graduate degree dissertation topics show

Mage-H1 and Mage-D1 regulation of the cell cycle and differentiation difference

Author: Ding
Tutor: LiuShaoJun
School: PLA Military Academy of Medical Sciences
Course: Cell Biology
Keywords: Mage-H1 Mage-D1 PC12 Cell cycle Cell differentiation
CLC: Q42
Type: Master's thesis
Year: 2008
Downloads: 26
Quote: 0
Read: Download Dissertation

Abstract


The traditional view, neurons can not be split, but observed in the previous work of our laboratory, multiple sources of mature neurons can be split, and with continuous photographic methods to capture to in mitosis all time separatist neurons, further with a variety of biological technology, to prove that it has various features typical of mature neurons in the division neurons. This shows, the splitting of neurons in the nervous system of universal significance. Why neurons from the cell cycle? Why, under certain conditions, can be split? Explore the molecular mechanism of which have important biological significance. Start from the study of two structurally similar but the function is relatively molecular the Mage-H1 and Mage-D1, investigate their possible role in the cell cycle, the regulation of cell proliferation and differentiation, so as to explain the molecular mechanisms of neurons from the cell cycle lay the foundation. In-depth study, we first dominant epitopes Mage-H1 and Mage-D1 predict. Mage-D1 protein N-terminal 53aa-295aa prokaryotic expression and preparation for Mage-D1 protein antibody to lay the foundation for further study its function. (Mage-H1 antibody preparation section will be set forth in our laboratory a Master thesis.) This study found that, after the induction of differentiation of PC12 cells, Mage-H1 upregulation, at the same time, Mage -D1 expression was lowered. This shows that the Mage-H1 may be involved in the differentiation of PC12 cells, maintain cell resting midterm play an important function; Mage-D1 may promote the proliferation of PC12 cells maintain cell dedifferentiation state to play an important function. Subsequently, we will be able to express the plasmid with the GFP-tag Mage-H1 transfected PC12 cells and found that the part of the expression the Mage-H1 of PC12 cells differentiation. The flow cytometry analysis showed the Mage-H1 transient overexpression obvious G0-G1 phase arrest of PC12 cells. Western-blot detection PC12 cells after transfection Mage-H1 cDNA24hrs, Cdk2 that were down-regulated, and this downward can last 72hrs. Furthermore, we obtained were screened by G418 exogenous MAGE-H1 in PC12 cells stably expressing clones. Flow cytometry analysis showed that compared with the native PC12, PC12 cells stably expressing Mage-H1 clone obvious G0-G1 phase arrest. Moreover, Mage-H1 PC12 cells stably expressing clones were induced by NGF easier to differentiate into neurons. In the yeast two-hybrid screening experiments, we find the interacting proteins Rit2 of Mage-D1. Rit2 active form through ERK and p38 MAPK pathway to promote the differentiation of PC12 cells. We speculate that, Mage-D1 through inhibition of the activity of the Rit2 PC12 cells dedifferentiated state.

Related Dissertations

  1. Study of Oridonin on SGC-7901 Cell Proliferation by Inhibitting Cell Cycle Proteins,R285
  2. Expression of Prolyl Isomerase Pin1 in Osteosarcoma and the Effect of Regulation on Cell Cycle,R738.1
  3. The Elective Toxic Effect of One Component Separated from Cobra Snake Venom on the Neuronal Cell and Its Possible Mechanisms,Q51
  4. Heme Oxygenase is Involved in Salinity-Induced Tomato Lateral Root Development and Its Relationship with Hydrogen Peroxide,S641.2
  5. The Involvement of Heme Oxygenase/Carbon Monoxide System in Cobalt Chloride-Induced Lateral Root Development in Tomato Seedlings,S641.2
  6. The Impact of Histone Deacetylase Inhibitor Trichostatin a on Proliferation and Apoptosis of Porcine Granulosa Cells,S828
  7. Study on the Cell Cycle of Liver Cells during Rat Liver Regeneration,Q253
  8. Effects of Different Immunization on the Development of Immune Organs,cell Proliferation and Expression of Apoptosis-relatived Gene in Chicken,S858.31
  9. Influences of Temperature on SCF/c-kit Prolifertation Pathway and Cell Cycle of Rat Spermatogonial Cells Cultured in Vitro,R698
  10. Amiloride Is Protective in PC12 Cells Via Autophagy Pathway,R329
  11. The Study on the Radiosensitizing Effect of Artesunate on Nude Mouse Transplanted with HeLa and Siha Cells of Cervical Cancer,R737.33
  12. Radiosensitizing Effect of Cathepsin L on Glioma Stem Cells,R739.4
  13. Study on Anticancer Effect of Two Novel Topoisomerase Inhibitors and Their Mechanisms,R96
  14. Erigeron Breviscapus and Its Active Component of Anti-lymphoma Effect,R285.5
  15. The Influence of Exogenous Hyaluronic Acid on Chondrogenic Differentiation of Rabbit Bone Marrow Mesenchymal Stem Cells,R329
  16. Effect of 5-Aza-CdR on the Biological Activity and Inhibitor of DNA Binding 4 (ID4) Gene Expression of Human Erythromyeloblastoid Leukemia Cell Line K562,R733.73
  17. The Expression of CyclinA、CyclinD1 and P27 in Different Situations of Astrocytes,R329
  18. Role of Ubiquitin B in 17-AAG-Induced Cell Cycle Arrest in HeLa Cells,R737.33
  19. β 2 -adrenergic receptor agonist clenbuterol on PC12 cell injury and its mechanism,R96
  20. Low-dose microcystin on rat liver cells proliferation effect and Its Mechanism,R363
  21. The Preminary Study about the Effect of Rosiglitazone on Proliferation Inhibit and Radiosensitivity of Human Nasopharyngeal Carcinoma Cell Line CNE-2,R739.63

CLC: > Biological Sciences > Physiology > Neurophysiology
© 2012 www.DissertationTopic.Net  Mobile