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Developed in the 1990s, resonance light scattering (RLS) is a new trace analytical techniques, simpler operation than the average fluorescence spectrometry, better selectivity, higher sensitivity, etc., the RLS technology have developed rapidly in recent years. Currently, RLS as a new analysis methods have been widely applied to the analysis of proteins, nucleic acids, cells, drugs, surfactants, inorganic ions and nanoparticles such. RLS technology to further research and development to analysis of the determination of a new system and new methods of research and one of the objectives pursued by the analysis of chemical workers. This dissertation is divided into five parts, the main contents are as follows: First, the basis for the development of resonance scattering spectroscopy, principles, quantitative analysis, and chrysin osthole, Paeonol and riboflavin and its main analysis method be summarized. Second, the use of resonance light scattering spectroscopy, based on pH ='s 3.8 HAc-NaAc buffer solution, sodium dodecyl sulfate (SDS) dialogue the of salicin (Chrysin)-Al 3 System Resonance Light Scattering significantly enhanced role, the establishment of a new method for the determination of wood butterfly chrysin, optimized experimental conditions, at 565 nm, the system RLS intensity and the concentration of chrysin linear relationship (r = 0.9942), linear the range of 0.05 to 2.8μg/mL a detection limit of 2 × 10 -4 μg / mL, and the recovery was 92.1 to 101.3%. Method for determination of the herbs Oroxylum Poplar, with satisfactory results. , PH = 5.0 of BR buffer solution conditions, beta-cyclodextrin Osthole Osthole - Alizarin Red by Resonance Light Scattering significantly enhanced role determination Cnidium osthole a new method, and by optimizing the experimental conditions obtained at 365 nm, the the system RLS intensity and osthole concentration of was a linear relationship (r = 0.9985), the linear range of 0.5 to 12.0μg/mL, a detection limit of 7.3 × 10 -3 μg / mL, the recovery was 95.6 to 105.1%. Determination on the herbs Cnidium osthole content, with satisfactory results. Fourth, the establishment of the determination of the content of Dan phenol (Paeonolum) resonance light scattering (RLS) spectroscopy. HAc-NaAc medium pH = 4.5 of Paeonol with cobalt (II), alizarin red (Alizarin) mainly by virtue of the hydrophobic interaction combined lead to significantly enhance the system by resonance light scattering; at a wavelength of 554 nm, RLS The degree of enhancement Paeonol concentration showed a good linear relationship (r = 0.9967). The linear range is 0.08 to 1.4μg/mL a detection limit of 3.6 × 10 -2 microg / mL. The method is simple, rapid and high sensitivity, for drug pharyngitis film Paeonol determination, with satisfactory results. Five - hydrochloric acid buffer solution, riboflavin (Riboflavin) and 1 - naphthol interactions that lead to the resonance light scattering intensity is significantly enhanced in the presence of sodium dodecyl sulfate, pH = 3.0 sodium citrate, pursuant to which the resonance light scattering method for rapid determination of riboflavin in tablets. In the λ = 540 nm at the maximum resonance light scattering intensity (ΔIRLS) and light scattering intensity and the concentration of riboflavin in the range of 0.2 to 3.0μg/mL showed a good linear relationship (r = 0.9925), and a detection limit of 2.38 × 10 -2 μg / mL. The method is simple, fast, high sensitivity for the determination of riboflavin in commercially available vitamin B2 tablets with satisfactory results.
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