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Electrochemical Biosensing Based on the Cytochrome P450 Composite Film Modified Electrode

Author: DaiChunYan
Tutor: FeiJunJie
School: Xiangtan University
Course: Analytical Chemistry
Keywords: Cytochrome P450 Direct electrochemical Electro-catalytic Biosensor Environmental endocrine disruptors
CLC: TP212.3
Type: Master's thesis
Year: 2011
Downloads: 47
Quote: 0
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Abstract


The cytochrome P450 (Cytochrome P450, CYP) are present in most of the living body, the generic name of a structure similar to a family of redox protease. Variety of P450 enzymes can catalyze a variety of types of reactions, it is not only the metabolism of drugs, pesticides, environmental endocrine disruptors foreign-derived substances, is also involved in a number of endogenous substances such as fatty acids, steroid hormones, prostaglandin synthesis, plays a very important role in the organism. Cytochrome P450 Electrochemical Biosensors is a research hotspot in recent years, because the method can simulate life forms, explore the direct electrochemistry and catalytic mechanism of the P450 in order to understand its mechanism of action in vivo, and help prepared in a highly efficient biosensors and biomedical devices. In this thesis, in order to achieve the cytochrome P450 enzyme direct electrochemical and electrocatalytic for the purpose of building several new types of composite materials as cytochrome P450 fixed material. These materials not only a favorable microenvironment for P450, P450 and between the electrodes for direct electron transfer has played a catalytic role. P450 embedded in these composites to achieve its direct electrochemistry, and the corresponding substrate exhibit good catalytic performance. This thesis work: using the good dispersion of the surfactant sodium dodecyl sulfate (SDS), acetylene black (AB) of the electrically conductive material uniformly dispersed in SDS solution to obtain a stable complex materials SDS-AB for the fixed cytochrome P450 6A1 (CYP6A1). The results showed that CYP6A1 was successfully immobilized in SDS-AB composite material, the composite material not only for CYP6A1 bionic micro-environment, and contributed greatly to CYP6A1 and electron transfer between the electrodes. SDS-AB/CYP6A1 modified electrode in 0.1 mol · L -1 phosphate buffer solution (PBS) with a pair of stable quasi-reversible redox peaks. The modified electrode substrate for CYP6A1 oxygen and progesterone exhibit good catalytic effect. PEG has good biocompatibility and dirt capacity are widely used in biomedical devices, biosensors. In this paper, the PEG 2000 as biosensors fixed material, the cytochrome P450 3A4 (CYP3A4) is fixed on the electrode surface, the PEG 2000 can provide a safe, stable protein micro- Setting, AB is passed as an electronic accelerator is introduced in the system. The studies show that CYP3A4 fixed to the composite system can be realized directly Electrochemistry, exhibit excellent electrochemical behavior. The use of the modified electrode detection of environmental endocrine disruptors atrazine, can be more sensitive electrochemical response. 3 Synthesis of a novel 2 - methacryloxy ethyl phosphate choline (MPC), and methyl acrylate, glycidyl methacrylate (GMA) phospholipid-based polymer P (GMA-co-MPC). Acetylene black can be uniformly dispersed in the polymer solution to form a stable suspension. CYP3A4 has been successfully fixed in the composite membrane. The results show that the polymers can be firmly fixed proteins in the membrane, CYP3A4 a biomimetic microenvironment, AB as the good performance of a conductive material can contribute significantly to the electron transfer. CYP3A4 fixed in the composite membrane showed a stable, sensitive electrochemical response, to achieve the direct electron transfer between the enzyme and the electrode. The modified electrode for the detection of environmental endocrine disruptors diethylstilbestrol, the linear range of 2.0 × 10 -7 -2.8 × 10 -6 mol · L -1 , a detection limit of 5.9 × 10 -8 mol · L -1 .

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CLC: > Industrial Technology > Automation technology,computer technology > Automation technology and equipment > Automation components,parts > Transmitter ( converter),the sensor > Biological sensors,medical sensors
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