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Labiatae mint genus spearmint spearmint (Mentha spicata L) grass. Its spicy, sweet, slightly warm, with qi, expelling wind and cold, anti-inflammatory, hemostatic, convergence of efficacy for the treatment of colds, Stomach pain, epistaxis, red eyes, sore hot scabies embolism. Ancient herbal medicine on spearmint describes the small but have mentioned. In addition, according to our preliminary pharmacodynamic study results, spearmint has anti-inflammatory, analgesic, anti-ulcer, anti-bacterial, anti-viral effect. The present study is based on 863 major national science and technology projects - spearmint soft capsules in the treatment of severe hemorrhoids \study using methylation method of processing the water-soluble portion of the sample to reduce its polarity, and applying a variety of separation techniques of extraction technology, macroporous resin D101, by column chromatography on silica gel, dextran gel (Sephadex LH-20) chromatography, etc. methylation chemical composition of the water-soluble part of the spearmint isolated to 15 monomer components, through the analysis of the physical and chemical properties and spectral data ( 1 H-NMR 13 C-NMR) with literature identified the structure of the 13 compounds were: citric acid trimethyl ester (trimethyl citrate, 1), protocatechuic acid methyl ester (methyl protocatechuate 2) Methyl vanillate (methyl vanillate, 3), sub-inosine (inosine, 4), uridine (uridine, 5) the carrots glycosides (daucosterol, 6), vanillin (vanillin, 7), 4 - hydroxyphenyl n-butyl (n-butyl-4-hydroxybenzoate, 8), AST (astragalin, 9), syringin (syringin, 10) the Adenosine (the adenosine, 11), rutin (rutin, 12), Quercetin-3-O-β-D-glucopyranoside glycoside (quercetin-3-O-β-D-pyranoglucoside, 13). The compound 1,2 and 3 reduced to an organic acid, followed by citric acid (citric acid, 1 '), protocatechuic acid (protocatechuic acid, 2'), homovanillic acid (vanillic acid, 3 '), and by high-performance liquid phase chromatography to verify that the product of these three compounds are methylated. Above compound 1 ', 2', 3 'and 4-13, the compounds 4,5 and 11 for the first time from the mint are isolated plants already known compounds; compound 1' ,7-10 ,12-13 for the first time from leaving spearmint isolated known compounds. The significance of this study is that: for the first time using the methylation of the chemical conversion process to reduce the polarity of the water-soluble portion of spearmint, and using a variety of chromatographic separation method to clear the chemical composition of the methylated product, and then found the spearmint Hong more reactive monomer components, also provides the material basis for the subsequent development.
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