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The Role of Peoniflorin on Organotypic Brain Slices Derived from Rat Substantia Nigra Treated with 1-methyl-4-phenylpytidinium in Vitro

Author: XuLong
Tutor: DongWanLi；KongYan
School: Suzhou University
Course: Neurology
Keywords: Parkinson's disease Rats Brain slice culture 1 - methyl-4 - phenylpyridine ion Paeoniflorin
CLC: R285.5
Type: Master's thesis
Year: 2008
Downloads: 77
Quote: 1
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Abstract

[Objective] To explore the rat substantia nigra organotypic brain slice culture method. Production methods. Explore MPP injury in rat model of Parkinson brain slices. 3. Observed brain slices paeoniflorin MPP damage substantia nigra dopaminergic neurons. [Method] 1. Election SD rats prepared in the substantia nigra organotypic brain slices (thickness of 350 μm). Millicell-CM microporous membrane brain slices in vitro culture techniques cultured brain slices, light microscope, HE staining and TH immunohistochemical reaction staining, morphology and TH positive cell count comparison with SD rats of the same age. Select good growth of brain slices with MPP were incubated 48h, the effects of different concentrations (0.1 mmol / L, 0.5 mmol / L and 1.0 mmol / L) MPP dopamine neurons in brain slices damage. Select well-grown brain slices MPP and Paeoniflorin, were incubated 48h, treated with different concentrations (1.0μmol / L 10.0μmol / L) paeoniflorin MPP damage dopaminergic neurons. [Results] culture 0 ~ 14 d organotypic brain slices substantia nigra cell volume and cell density were less than the same period in the body and development of the substantia nigra structures; vitro culture with the same period in the body and development of the substantia nigra structure comparison, TH tends childish reaction cell connections between cells also appeared later, both TH cell count was statistically significant (P lt; 0.01). Substantia nigra organotypic brain slices edge blur 2.MPP role, the cell structure is unclear, cell density compared with the same period from the substantia nigra brain slice culture group (control group); TH reaction cell density compared with the normal control group, the cells connection between processes reduce, particularly evident in the MPP concentration group and the high concentration group; effect of various concentrations of MPP the TH reaction cell count with normal control group was statistically significant (P lt; 0.05). Paeoniflorin and MPP were incubated under the cell structure of the substantia nigra organotypic brain slices less clear, but around the brain slice off the necrotic cell mass was significantly less than the the only MPP role in the substantia nigra brain slices (control group) and cell density. concentration MPP group the cell structure incubation of high concentrations of paeoniflorin clearer; connections between cells increased significantly compared with the control group TH reaction; incubation of high concentrations of paeoniflorin TH the reaction cell counts compared with control group was statistically significant (P lt ; 0.01). [Conclusion] 1. Substantia nigra brain slices in vitro culture similar to the structural development of the substantia nigra in the growth process, the substantia nigra brain slices in vitro culture developmental lag in the substantia nigra in postnatal development structure. 2 in the rat substantia nigra organotypic brain slice culture is a good method to study the pathogenesis of Parkinson's disease. (10.0μmol / L), high concentrations of paeoniflorin rat substantia nigra brain slices dopaminergic neurons have a protective effect.

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