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Study on Angiogenesis of Joint Synovial and Lung Lesions of Collagen-induce Arthritis with Interstitial Lung Disease Affected by Bitonglin Pellet

Author: FanRenZhu
Tutor: WangYue
School: Nanjing University of Traditional Chinese Medicine
Course: Chinese medical science
Keywords: Bitongling particles Arthralgia Lung paralysis Rheumatoid Arthritis Interstitial lung disease Angiogenesis
CLC: R285.5
Type: PhD thesis
Year: 2011
Downloads: 84
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Abstract


Purpose: the overall concept from the Chinese, according to TCM arthralgia skin paralysis endless, complex sense in evil, homes in the lung, limb paralysis and lung paralysis associated theories Bitongling particles observed on collagen-induced arthritis synovial and lung pathological angiogenesis effect, provide experimental basis for clinical application. Methods: In the currently accepted type Ⅱ collagen-induced arthritis (CIA) model, based on the use of bleomycin induced interstitial lung disease (ILD), exploring ways to improve the rat type Ⅱ collagen-induced arthritis with bleomycin adriamycin induced interstitial lung disease complex model (CIA-ILD). 2 naked eye the overall status of the rats, activity behavior, joint swelling, respiratory status. 3 With reference to the literature method arthritis assessment scores (AI) 4. Measured with dual rear foot drainage volume change. 5 HE staining, rats were observed synovial and lung pathological changes, and score. 6 using F Ⅷ factor (factor Ⅷ related antigen) antibody immunohistochemical markers, observed the synovium and lung microvascular density (MVD) changes. 7 Real-time quantitative PCR (Real-Time PCR, RT-PCR) method for detecting particles observed in each group Bitongling synovial tissue and lung tissue IL - 17mRNA, IL-1βmRNA, TNF-αmRNA, VEGFmRNA gene expression. 8. Information and data to x ± s said that the application 2-ΔΔCt analysis real-time PCR data, when the target gene expression ≥ 2-fold, increased expression of the gene that, if ≤ 0.5 times that gene expression decreased. The remaining data applications SPSS17.0 statistical software analysis, the groups were compared using analysis of variance, with P lt; 0.05 was considered statistically significant. Results: 1.CIA-ILD experimental model rats overall state of water, food intake, shiny fur, reduced activity behavior than the normal group; joint swelling, breathing accelerated compared with the normal group. Model unit volume comparison bipedal rats with arthritis score the first 21 days compared with modeling modeling the first seven days was significantly increased (P lt; 0.05). Model group the first seven days of mesothelial synovial thickening, inflammatory cell infiltration; modeling 14 days mesothelial synovial thickening, increased inflammatory cell infiltration; modeling the first 21 days of mesothelial synovial thickening, inflammatory infiltrating cells are more aggravating. Synovial pathology severity score the first 21 days compared with modeling modeling the first seven days was significantly increased (P lt; 0.05). Seventh day of the modeling and model group showed mild to moderate alveolar wall thickening, interstitial lymphocyte plasma cell infiltration of inflammatory cells, local bleeding, modeling the first 14 days of severe alveolar wall thickening, interstitial lymphocytic infiltration of plasma cells, modeling day 21 shows a large number of lung tissue inflammatory cell infiltration patchy distribution, particularly evident in the bronchial lesions around. Widened alveolar septum congestion, local alveolar epithelial cell proliferation, some alveolar macrophages were seen in clusters integrated group, fibroblasts and fibroblast proliferation, pulmonary fibrosis alveolitis compare modeling modeling first 21 days compared with the first seven days increased significantly (P lt; 0.05). Histopathology Section 7 days, 14 days, 21 days observed, synovial and lung lesions appear and progress of modeling success. Two experimental model rats arthritis swelling significantly, the treatment group has a different degree of joint swelling lessened, arthritis scores were lower than model group to Bitongling high dose group and the TWP Group Performance more significant (P lt; 0.05). Model group increased synovial histopathology score, synovial tissue synovial cell proliferation, multilayer above, arrange evacuation disorders, inflammatory cell infiltration, gathered. Bitongling high dose group, middle dose group Bitongling, TWP pathology group improved significantly, Bitongling low dose group improved. Model seen in lung tissue inflammatory cell infiltration, alveolar septal congestion widened alveolar hemorrhage, fibrosis occurs. Bitongling high dose group, middle dose group Bitongling, TWP pathology group improved significantly (P lt; 0.05), low-dose group Bitongling improved. Three experimental model rats synovial tissue and lung tissue MVD increased number of relatively normal group, low dose group Bitongling high school and TWP synovial tissue of rats and lung tissue compared with the model group, the number of MVD decreased (P lt; 0.05). 4 experimental model group than in the normal group IL-17mRNA synovial expression ≥ 2-fold, gene expression in rat synovial tissue and lung tissue increased. Bitongling High dose group than in model group IL-17mRNA expressed in synovial ≤ 1/2 times the gene expression decreased. GTW group than in the untreated group IL-17mRNA expression in synovial fold ≤ 1/2, gene expression decreased. Model rats lung tissue expression of IL-17mRNA ≥ 2-fold higher than the normal group, increased gene expression. Bitongling high dose group rat lung tissue expression of IL-17mRNA than the model group ≤ 1/2 times the gene expression decreased. TWP lung tissue expression of IL-17mRNA than the model group ≤ 1/2 times the gene expression decreased. 5 experimental model rats synovial tissue expression of IL-1βmRNA ≥ 2-fold higher than the normal group, increased gene expression. Bitongling high dose group and TWP rat synovial tissue expression of IL-1βmRNA than the model group ≤ 1/2 times the genes downregulated. Model of lung tissue expression of IL-1pmRNA ≥ 2-fold higher than the normal group, gene expression increased Bitongling high dose group and TWP lung tissue expression of IL-1βmRNA than the model group ≤ 1/2 times downregulated. 6 experimental model rats synovial tissue and lung tissue expression of TNF-αmRNA ≥ 2-fold higher than the normal group, increased gene expression. Bitongling dose group and high school groups TWP synovial tissue of rat TNF-αmRNA expression than the model group ≤ 1/2 times the genes downregulated. Model of lung tissue TNF-αmRNA gene expression relative to the normal group increased Bitongling High dose group and TWP lung tissue TNF-αmRNA than the model group ≤ 1/2 times the genes downregulated. 7 experimental model rats synovial tissue expression of VEGFmRNA ≥ 2-fold relative to the normal group, increased gene expression. Bitongling high dose synovial tissue of rats in model group than VEGFmRNA ≤ 1/2 times the genes downregulated. Model group relative to normal lung tissue VEGFmRNA group ≥ 2 fold increased gene expression, high school Bitongling lung dose group compared with model group VEGFmRNA ≤ 1/2 times the genes downregulated. TWP rat VEGF mRNA in lung tissue compared with model group ≤ 1/2 times the gene expression decreased. Conclusion: To investigate Bitongling particles of rheumatoid arthritis affecting the joints and lung disease, the first in a CIA-ILD modeling work. Histopathology Section 7 days, 14 days, 21 days synovial and lung lesions appear and progress. 2 arthralgia Bitongling particles on an experimental model of rheumatoid arthritis synovial CIA-ILD rats and lung inflammatory pathology has two parts inhibitory effect of high low-dose Individually, senior dose group becomes more apparent. 3 Bitongling particles on the CIA-ILD rat synovial tissue and lung tissue inflammatory angiogenesis based on the pathological changes of microvessel density (MVD) has a significant role in mitigation, high school dose Jieyou good results. 4 Bitongling particles allows CIA-ILD synovial tissue and lung two parts, four inflammatory cytokine gene expression in angiogenesis down, high school dose effect is obvious.

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