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Shan’aoxiongtingheji on Airway Inflammation in Asthmatic Rats Experiment

Author: DaiPing
Tutor: YangRenXu
School: Chengdu University of Traditional Chinese Medicine
Course: Chinese medical science
Keywords: Bronchial asthma Xuanfei stasis phlegm law Sanniuxiongting Mixture Airway inflammation T-betmRNA GATA-3mRNA IFN-γ IL-4
CLC: R285.5
Type: PhD thesis
Year: 2009
Downloads: 133
Quote: 1
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Abstract


The purpose of the first part of the animal experiments: explore three-bend Shenxiong Draba transcription factor T-bet and GATA-3 and serum IFN-γ, IL-4 affect Mixture of asthmatic rats. Methods: 100 healthy SD rats were randomly divided into control group (control group), asthma model group (model group), figuratively, Xiong Draba mixture of high-dose group (referred to as the high-dose group, the same below), dose group and low dose group, dexamethasone group (referred to m group) of six groups. Blank group modeling with saline, intraperitoneal injection of the remaining five rats with OVA hydrogenation alumina, only use the same amount of saline (control group) from the first 3 weeks, 1% OVA solution atomization excitation (the remaining five groups) to stimulate 1h before with the same volume of saline (control group and model group), the the different concentrations bend Xiong Draba mixture (large, in the low-dose group) gavage, intraperitoneal injection of dexamethasone weeks (ground m group). After 3 weeks the rats were sacrificed to take the right lung measurement the lung tissue transcription factor T-betmRNA, GATA-3 mRNA using RT-PCR method, the serum by ELISA measurement of IFN-γ, IL-4 concentration. Improve understanding of airway inflammation by lung tissue biopsy. Results: The model group expression of GATA-3 mRNA and IL-4 concentrations were significantly increased, the T-betmRNA, IFN-γ concentration decreased, T-betmRNA/GATA-3mRNA of IFN-γ/IL-4 ratio decreased; blank group The ground rice group, the high-dose group, middle dose group and low dose group expression of GATA-3 mRNA and IL-4 concentration decreased to varying degrees T--betmRNA, IFN-γ concentration T-betmRNA/GATA-3mRNA IFN-γ / IL-4 ratio increased to varying degrees, to the effect of high-dose group, and m group significantly. Conclusion: The the three bend Xiong Draba mixture can enhance T-betmRNA expression in rat lung tissue, serum IFN-γ levels, or elevated T-betmRNA/GATA-3mRNA of IFN-γ/IL-4 ratio alleviate asthmatic rats airway inflammation, and improve the quality of life of rats. Provide an experimental basis for three the bend Shenxiong Draba mixture in treating asthma. Part of the purpose of the second part of the clinical observation: To observe the clinical efficacy of the three the bend Shenxiong Draba mixture and Analysis of the mechanism of the party against the disease. Methods: 61 cases in mild to moderate chronic persistent asthma period, TCM is phlegm lung patients as objects, were randomly divided into a treatment group (c bend Gung Draba Mixture joint 80ug/4.5ug Symbio can Turbuhaler) and control group (160ug/4.5ug Symbio can Turbuhaler) were observed before and after treatment improvement of symptoms. The frequency of asthma attacks before and after treatment, Ventolin number of individual symptom scores, scores of TCM, the overall card Hou improve integral indicators ACT scores were determined. Results: Statistical results of the treatment and control groups before treatment, the patient's age, sex, duration and severity, symptom score, frequency of asthma attacks, the use of Ventolin Views no statistically significant difference (P gt; 0.05), description of the two groups of patients comparable. 4-8 weeks after treatment in both groups significantly in patients with reducing asthma attack frequency; Ventolin frequency of use, increased ACT score; statistics showed that the two groups after treatment efficacy was no significant difference (P gt; 0.05). Can improve the patient's symptoms, TCM individual symptom scores, scores of TCM, TCM whole card Hou integral statistical treatment in both groups, a significant difference (P lt; 0.05), the treatment group than the control group. Conclusion: the three bend Xiong Draba agent can reduce the amount of Symbicort Turbuhaler effectively improve the patient's condition and reduce the patient's asthma attack frequency and the frequency of use of Ventolin, reduce airway hyperresponsiveness to reduce the clinical signs and symptoms, improve the patient's quality of life, temporarily not found any side effects, Chinese medicine therapy is expected to be effective treatment of asthma.

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