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Newcastle disease is an acute, highly contagious infectious disease caused by NDV . The disease is one of the major epidemics against the development of the poultry industry , the effects of drugs on the NDV , application of herbal medicine has broad prospects for prevention and treatment of viral diseases in Chicken . Honeysuckle compound preparation honeysuckle , forsythia , Houttuynia , Astragalus , Atractylodes , Licorice prescriptions made ??by the laboratory screening has been the subject of another part of the test prove honeysuckle compound preparation has to raise chicks peripheral blood of specific and nonspecific immune function role. The test is based on a 21 day-old chicks for the study, by flow cytometry technology , cell culture technology , MTT assay , immunoenzyme staining method and radioimmunoassay method comprehensive and systematic study of the honeysuckle compound preparation on artificially infected with the immune NDV the chicks immune organs ( thymus , spleen and bursa ) T lymphocytes and their subsets , B lymphocytes affect the impact ; lymphocyte proliferation of immune organs ( thymus , spleen and bursa ) function ; organs ( bursa, spleen , thymus ) antibodies (IgG, IgM) to generate the impact of the number of cells and splenic T lymphocyte cytokines (interferon - γ , interleukin- 2 ) , designed to reveal honeysuckle compound preparations on artificially infected with NDV the chicks immune organ cellular immunity and humoral immune function and its mechanism . The study results showed that : the honeysuckle compound preparation to improve artificially infected with NDV the chicks immune organs thymus and spleen CD3 , CD4 T - lymphocyte percentage , CD4 / CD8 T lymphocytes value rises , improve immune organs, spleen and bursal B lymphocyte percentage , improve the ability of the thymus and spleen T lymphocyte proliferation and spleen and bursal B lymphocyte proliferation , immune organs bursa, spleen , thymus, IgG , IgM number of producing cells , and IFN-γ can promote spleen lymphocytes and IL-2 secretion , thereby enhancing the body 's cells and humoral immunity , indirect that honeysuckle compound preparations with certain anti-NDV .
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