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In this paper, ISSR technique, Liaoning Province Natural Korean Pine study the genetic diversity . Were screened out 13 amplified bands abundant polymorphism primers . The DNA samples were chosen from different regions of Liaoning Province, five natural pine populations in this study using a modified CTAB method pine genomic DNA, this method extracted DNA content and high purity to meet a large number of PCR amplification needs. DNA was then purified to establish a stable , repeatable pine ISSR-PCR reaction system . This paper studies the genetic diversity of the results : The total site number 116 , of which 90 polymorphic loci ; percentage of polymorphic loci was 77.59%; Shannon index average was 0.4461; Nei index average was 0.3005 ; From this red pine has a high genetic diversity. The total genetic diversity among populations of 19.81% of the genetic diversity , genetic diversity within populations accounted for 80.19% of the genetic diversity . Thus, the genetic diversity of pine forests mainly from within the group . Under this five pine populations genetic distance , using its UPGMA cluster analysis to obtain tree . It can be concluded Fengcheng sand hillock, Kuandian Lin Chuan , Kuandian Baishilazi together in a group , Benxi and Fushun New Bin Pali Lookout Hill Gang together in a group, the classification results positively correlated with the actual location .
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