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Pagoda tree (Sophora japonica L.), small to medium tree, is the excellent timber, medicinal, edible and ornamental trees. Sophora japonica naturally distributed in China, Korea and Japan, cultivated widely introduced in other parts of the world. In this study, Sophora japonica Hangzhou, Zhejiang, Hubei Xiaogan, Xi'an, Xianyang, Shaanxi, Beijing, Henan and Shandong Taian Puyang seven groups, each randomly selected 130 samples per plant as a seed strain, its seed length, width, aspect ratio, surface area, volume and grain weight were observed six traits analysis using Nested design analysis of variance, correlation and clustering and other statistical analysis, analysis of different phenotypic variation and rules in an attempt to Sophora japonica Genetic diversity studies provide quantitative indicators for the evaluation of genetic resources Sophora japonica, provide the basis for the conservation and utilization. In Beijing Fengtai nursery base collection and preservation Sophora japonica varieties and clones as test material to investigate Sophora japonica DNA extraction and SRAP-PCR reaction system the best, for further Sophora japonica SRAP Analysis of genetic resources at the molecular level differential varieties and clones and their genetic relationship to provide a scientific basis. The main results are as follows: 1 Sophora japonica seed morphological traits varying degrees of variation, the coefficient of variation ranging from 5.24% -14.57%; six phenotypic traits among populations and within populations have reached a very significant level; six traits mean phenotypic differentiation coefficient was 53.11%, indicating slightly higher genetic variation among populations within populations; seed morphological traits and geographic latitude and longitude degree of correlation is low. Using the results show 7 Sophora japonica populations can be divided into three categories: 1) Tyan groups; 2) Hubei Xiaogan and Beijing groups; 3) Hangzhou, Xi'an, Xianyang and Henan Puyang clustered into one group. Study found no obvious geographical Sophora japonica seeds Variation may be caused by long-term artificial cultivation, but also may be due to incomplete sampling or discontinuities caused. Two orthogonal experiment to determine the optimum SRAP-PCR Sophora japonica reaction system (20 uL): Taq polymerase 1.5 U, dNTPs 0.30 mmol / L, template DNA 90 ng, Mg 2 2.0 mmol / L, primer 0.3 umol / L, ddH 2 O complement to 20 uL. 3 from 120 pairs of primer combinations screened out 12 pairs of PCR product bands were clear, highly polymorphic primer combinations for Sophora japonica varieties and clones Molecular recognition of kinship. 12 pairs of primers for PCR amplification are concentrated in bands between 100bp-2000bp. In the obtained 430 amplified bands, 415 polymorphic loci polymorphic loci ratio was 96.60%, of which four pairs of primers polymorphic reached 100%. The results also show that, SRAP can detect Sophora japonica varieties and clones of multiple genetic loci. Using Em9-Me12 primer combinations can distinguish between good and different varieties of Sophora japonica clones, and the establishment of SRAP fingerprints of Sophora japonica. 4 Sophora japonica varieties and clones of the genetic similarity coefficient (SM) is 0.68-0.89, with an average similarity coefficient was 0.73, indicating Sophora japonica varieties and clones similarity between relatively high, relatively narrow genetic base. May be due to the historic pagoda tree cultivation, long-term result of artificial cultivation, there may be species selection and clonal selection range is relatively small, resulting in Sophora japonica varieties and genetic distance between clones is relatively small. Based on genetic similarity coefficient SM, on a non-weighted arithmetic average method for pairing Sophora japonica varieties and clones of cluster analysis, the similarity coefficient 0.76, will be divided into four categories, and the second category is the 4th and Sophora japonica 35 Number; third category Butterfly Cassia (S. japonica var.oligophylla Franch); fourth category Zhaohuai (S. japonica var. pendula Loud.), Caozhou the 2nd (S. japonica'Caozhou 2 ') and gold Ye Guohuai (S. japonica f. flavi-rameus); others are first class, first class can be divided into two subgroups; Butterfly Cassia (S. japonica var.oligophylla Franch) clustered into the third category alone, suggesting that different blade gap between relatively large.
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