Dissertation > Excellent graduate degree dissertation topics show

Study of Muskmelon and Watermelon by Transferring Antibacterial Peptide Gene Gnk2-1 of Ginkgo Biloba

Author: GaoNingNing
Tutor: ZhangXian
School: Northwest University of Science and Technology
Course: Olericulture
Keywords: Melon Watermelon Cotyledons Efficient regeneration Antimicrobial peptide genes Agrobacterium-mediated transformation Resistance
CLC: S652
Type: Master's thesis
Year: 2011
Downloads: 41
Quote: 0
Read: Download Dissertation

Abstract

Melon (Cucumis melo L.) and watermelon (Citrullus L.) belong to the Cucurbitaceae, the fruits of high economic value, are widely distributed in the world within a wide range of cultivated area and the majority of However, the process of growth and development in melon and watermelon, and often would be subjected to pest impact, especially wilt and anthracnose, a serious impact on the yield and quality of melon and watermelon. Many fine varieties have been bred by conventional breeding methods, but the improvement is more difficult for certain traits, genetic engineering improvement has become a direct and effective means of melon and watermelon, however efficient tissue culture and plant regeneration technology of transgenic technology a prerequisite. Antimicrobial peptide protein extracted from ginkgo seeds of melons Fusarium oxysporum (Fusarium oxysporum), anthrax (Colletotrichum orbiculare) and other fungi have a strong inhibitory effect. The test systematically studied the melon and watermelon cotyledons efficient genetic transformation system, the establishment of the regeneration and genetic transformation system for fast, efficient breeding of melon and watermelon, then the use of Agrobacterium-mediated transformation ginkgo antimicrobial peptide gene Gnk2 ^{- 1 Import expressed in melon and watermelon, melon and watermelon quality improvement and increased resistance to genetic engineering in the future lay the foundation. The main findings are as follows: 1. Trial established the cotyledons efficient regeneration system. Melon FY1, HY2 cotyledons were used to study the impact of different hormone combinations and ratios on melon regeneration. The research results show that: the FY1 cotyledon regeneration rate under the same conditions in HY2 cotyledon regeneration rate, its cotyledon callus and adventitious bud induction medium MS BA2.0mg · L ^{-1 IAA0 .1 mg · L ^{-1 , sprouting rate of more than 95%; Best bud elongation medium for best rooting medium MS KT0.2mg · L ^{-1 ; 1/2MS IAA 0.1 mg · L ^{-1 . 2. Watermelon cotyledons efficient regeneration system. To the watermelon varieties 134-2 standard, the Beijing parent -2 cotyledon material to study the impact of different hormone combinations and ratios on the regeneration of watermelon. The study results show that the cotyledons efficient regeneration system established: 134-2 standard varieties of watermelon cotyledons best medium for adventitious bud induction MS 6-BA2.0mg · L ^{-1 IAA0.2mg · L < sup> -1 , budding rate of up to 96%, than the parent -2 cotyledons budding high in Beijing; the shoot elongation best medium for MS KT0.2mg L ^{-1 ; best rooting medium The of 1/2MS IAA0.1 mg · L ^{-1 . 3. Optimize genetic transformation system for melon and watermelon. Melon of FY1 and watermelon 134-2 standard cotyledon explants, screening card kanamycin sensitivity test results show that the concentration of kanamycin kanamycin on the growth and differentiation of the melon and watermelon explants strong inhibitory effect, suitable screening pressure ^{100 mg · L -1 . The cotyledons preculture 2d, in the the bacterial concentration OD600 value of 0.5, and the infected explants around 10min, co-cultured 3d, the highest conversion efficiency. Acetosyringone (AS) on melon and watermelon conversion efficiency was less affected. Using Agrobacterium-mediated transformation ginkgo antimicrobial peptide genes Gnk2 ^{-1 transferred to the melon and watermelon, a preliminary identification by PCR-positive transgenic melon plants, detached leaves of transgenic plants connected bacteria test, the plants wilt resistance has been enhanced, the incidence of retardation.}}}}}}}}}}

Study of Muskmelon and Watermelon by Transferring Antibacterial Peptide Gene Gnk2-1 of Ginkgo Biloba

Abstract

Related Dissertations