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Poa (Poa L.) plants gramineous perennial or annual herb, widely distributed in temperate and cold regions, a class of great application value turfgrass. The trial Gansu Province collected eight wild bluegrass germplasm materials, moisture, temperature, salt stress on seed germination characteristics and NaCl Stress on Physiological Features to explore the wild bluegrass seed germination appropriate moisture, temperature, screening salt-tolerant germplasm materials for the Introduction of the wild bluegrass domestication and breeding of salt-tolerant varieties provide the basis. The main findings are as follows: 1. Increase of stromal water content, the eight wild bluegrass seed germination rate, germination potential, germination index, germ length, radicle length and fresh seedling weight showed first increased, then decreased trend ; Kentucky bluegrass (Sunan) the germination rate, germination potential 5% moisture content in the matrix and germination index, seven other material 10% moisture content in the matrix, and significantly higher than the other treatment (P lt; 0.05 ); 10% moisture content in the matrix, small drug bluegrass and hard bluegrass germ longest six other material germ maximum moisture content of 15% in the matrix; radicle 5% moisture content in the matrix, the longest; fresh Miao matrix water content of 10% the most weight. 2.10 ° C 3 5 ° C constant temperature range, with increasing temperature, eight wild bluegrass seed germination rate, germination potential, germination index, germ length, radicle length and fresh seedling weight performance the first increase after declining trend. Small medicine bluegrass wave Laval bluegrass and Kentucky bluegrass (Gannan) 20 ° C when the germination rate, germination potential germ radicle longest fresh seedlings heaviest; Kentucky bluegrass (Xinglongshan), Kentucky bluegrass (Sunan), Poa annua (Weiyuan), Kentucky bluegrass (Tianzhu) and hard bluegrass at 25 ℃, 15 ℃ the germination rate, germination potential and germination index of the highest, longest germ; small drug Poa When the highest germination index; wave cutting bluegrass 25 ° C at 20 ℃ seven other wild bluegrass radicle longest; Kentucky bluegrass (Xinglongshan), Kentucky bluegrass (Sunan) and wave cutting bluegrass 25 ° C the other 5 Wild Poa material in 20 ° C heaviest seasonal seedlings. 10 2 5 ℃ alternating temperature index value is generally greater than 5 2 0 ℃ alternating temperature. 3 seed germination of salt tolerance studies have shown that 50 mmol · L -1 low concentrations of NaCl stress wave cutting bluegrass and 25 mmol · L -1 low concentration of Na2SO4 Stress grass bluegrass (Xinglongshan), Kentucky bluegrass (Tianzhu), small medicine bluegrass, hard bluegrass, wave cutting bluegrass, Kentucky bluegrass (Gannan) relative germination rate, relative germination potential were significantly high than that of the control (P lt; 0.05). Radicle length and fresh seedling weight decreased with the increase of NaCl and Na2SO4 concentration. Salt stress on radicle growth greater than the impact of germ growth; greater than the effects of NaCl on seed germination the Na2SO4. 6 indicators fresh seedling weight average salt tolerance threshold and salt tolerance limit, radicle length The average minimum salt tolerance threshold and salt tolerance limit. Seed germination of salt tolerance as follows: small drug bluegrass gt; wave cutting bluegrass gt; Kentucky bluegrass (Sunan) gt; hard bluegrass gt; Kentucky bluegrass (Weiyuan) gt; Kentucky bluegrass (Gannan) gt; grass Poa (Tianzhu) the gt; Kentucky bluegrass (Xinglongshan). With NaCl concentration increased, the Kentucky bluegrass (Weiyuan) hard bluegrass, wave cutting bluegrass and Kentucky bluegrass (Tianzhu) the four material leaf chlorophyll content and soluble protein content decreased; Free Proline and malondialdehyde content increases gradually, gradually increased peroxidase activity; superoxide dismutase and ascorbate peroxidase activity was first increased and then decreased, 200 mmol · L -1 material superoxide dismutase activity were the strongest, 200 mmol · L -1 Poa annua (Weiyuan) and wave cutting bluegrass activity of ascorbate peroxidase the strongest, 150 mmol · L -1 hard bluegrass and Kentucky bluegrass (Tianzhu) ascorbate peroxidase activity strongest. Comprehensive Evaluation wave cutting precocious seedlings of Salt Tolerance strongest, followed by hard bluegrass and Kentucky bluegrass (Weiyuan), Kentucky bluegrass (Tianzhu) salt tolerance weakest.
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