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Gene mapping (gene mapping) is to determine a specific location of the genes on the chromosome and gene arrangement of the relative distance between , according to the genetic location data on each chromosome of the gene loci have been found drawn into the genome . The establishment of genetic maps requires a sufficient number of polymorphic markers , and traditional genetic markers is difficult to achieve the desired effect, greatly limiting the genetic map of the establishment . SSR markers used in this study as a genetic linkage map of molecular markers , microsatellite DNA markers on chromosomes are widely distributed, there is a wealth of polymorphism, and analyze the cost is relatively low. The materials used in this study by the Chinese Academy of Agricultural Sciences Key Laboratory of Oil Crop Biology, provided the material through a continuous trait selfing and selection , has received relatively pure grain weight genetic strains and large grain pellets strains . On the basis of the material , the authors used two grain weight disparity of hybridized strains and hybrids identification for real , and then identify the true selfing generation hybrids F2 population , which is the study of the F2 population object. First find polymorphism between the parents and SSR markers , use of these markers for marker genotypes F2 population to detect and analyze the data . Published from Brassica SSR primers selected to cover the entire genome of 551 rapeseed right, these primers detected polymorphism between the parents , a total of 107 pairs of primers detected polymorphism specificity ratio was 19.4% , 144 were detected specific bands . From 107 pairs of SSR primers amplified better to pick out 48 right, right a capacity of 190 individuals were genotyped F2 populations are marked . With Joinmap3.0 on analysis of the data obtained , of which 25 % of the marks showed a significant segregation , bias pellets parents have five (41.7%) , parents tend to have large grain 7 , accounting for 58.3% . And got five linkage groups , the integration of 16 SSR markers , covering a total length 295cM, the average distance between markers was 26.8cM. The resulting linkage map and the establishment of SSR molecular marker system for the follow-up experiments to lay a foundation .
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