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Primary Study on the Influence of β 3GnTs Expression Induced by As2O3 in Breast Cancer Cells and the Relationship between β 3Gnt8 and Transcription Factor C-jun

Author: ZhangHuanPing
Tutor: WuShiLiang；ZhouYingHui
School: Suzhou University
Course: Biochemistry and Molecular Biology
Keywords: breast cancer arsenic trioxide(As2O3) apoptosis c-jun β3GnTs
CLC: R737.9
Type: Master's thesis
Year: 2011
Downloads: 7
Quote: 0
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Abstract


Object Observe the inhibition of As2O3 （arsenic trioxide） with different concentrations on breast cancer cells growth, the apoptosis inducing function on the cell and the effect onβ3GnT2 andβ3GnT8 expression with different concentrations arsenic trioxide. Initially discuss the mechanism of As2O3 inducing apoptosis. It is also reported that there have been some relationships between glycosyltransferases and the transcription factor c-jun. This thesis also aims to study the relationship of them in breast cancer cells.Methods: The effect of As2O3 on the growth of breast cancer cell line MDA-MB-231 and MCF-7 was observed after applying different concentrations As2O3 in vitro. The inhibitory effect of As2O3 on the cell proliferation was investigated with 3-（4,5-dimethyl2thizazol-2-yl）-2,5-diphenyl tetrazolium bromide（MTT） and Hoechst33258; RT-PCR was adopted to detect mRNA expression ofβ3GnT2 andβ3GnT8. Western Blot was used to detect express status of related protein. Chromatin Immunoprecipitation was used to detect the relationships ofβ3GnT2 gene,β3GnT8 gene and transcription factor c-jun. Immunoprecipitation was used to detect the orientational expression of transcription factor c-jun, andβ3GnT2 andβ3GnT8 in breast cancer cells.Results: Compared to control group,different concentration As2O3 could induce the breast cancer cells apoptosis, and its inhibition strengthened along with dosage increasing, assuming the dose dependence. There was a significant difference of mRNA expression among anyβ3GnT2,β3GnT8 group and the negative control. The protein express status detected by Western Blot was in accordance with the level of mRNA.β3GnT2 andβ3GnT8 gene can be amplified in the product of Chromatin Immunoprecipitation. As immunofluorescence assay indicated, andβ3GnT2,β3GnT8 are expressed in the breast cancer cells and had the overlapped part.Conclusion: As2O3 can exert obvious inhibitory effect on MDA-MB-231 and MCF-7 cell growth in vitro.The inhibitory effect increases with dosage escalation and shows dosage dependence.As2O3 can induce the MDA-MB-231 and MCF-7 cell apoptosis,also the apoptosis effect and the dosage assume the positive correlation. As2O3 regulatsβ3GnT2 andβ3GnT8 expression down. We suggested transcription factor c-jun may have influence on the expression ofβ3GnT8. Transcription factor c-jun may have influence on the expression ofβ3GnT8.

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CLC: > Medicine, health > Oncology > Genitourinary tumors > Breast tumor
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