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The Effect of PPA on the Expression of TLR4、Act1、A20 and Proliferation in Human Bronchial Epithelial Cells

Author: YangHuiHui
Tutor: WangLiLi
School: Central South University
Course: Medical Microbiology
Keywords: PPA TLR4 Act1 A20 Cell Cycle
CLC: R965
Type: Master's thesis
Year: 2011
Downloads: 26
Quote: 0
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Abstract


Objective : Toll -like receptors (TLRs) are cell surface recognition of a pathogen -associated molecular patterns pattern recognition receptor family of innate immunity in the airway and acquired immunity play an important role. TLRs expression defects or poor response often leads to chronic airway infection . Actl IL-25 signaling pathway is an important signaling molecule , promoting Th2 inflammation ; A20 from the immediate early response gene encoding NF-κB signaling pathway as a potential inhibitor. This study intends to apply to import natural TLR4 ligand containing PPA, through its intervention allergen -induced or viral infection of the bronchial epithelial cells , observe its effect on airway epithelial TLR4, Act1, A20 , and proliferation of , in order to explore the PPA in the airway disease treatment in rats. Methods : HBECs after OVA sensitization induction or RSV infection source , then PPA intervention by flow cytometry HBECs spoon cell cycle by fluorescence quantitative PCR TLR4, Actl and A20 mRNA expression differences . Results: 1 cell cycle : PPA intervention caused OVA treated or RSV infection group HBECs S phase cells increased (P lt; 0.05). 2, TLR4: PPA intervention caused OVA treated group HBECs TLR4 mRNA expression was significantly increased (P lt; 0.01); caused by RSV treatment group HBECs TLR4 mRNA expression increased (P lt; 0.05). 3, Actl: PPA intervention caused OVA treated group HBECs Act1 mRNA expression significantly decreased (P lt; 0.01); caused by RSV treatment group HBECs Act1 mRNA expression was significantly increased (P lt; 0.01). 4, PPA intervention caused OVA treated or RSV infection group HBECs A20 mRNA expression was significantly increased (P lt; 0.01). Conclusion : 1, PPA can significantly promote the intake of epithelial cell proliferation, and can interfere with OVA treatment or inhibition of RSV infection of airway epithelial cell proliferation. 2, PPA intervention can increase HBECs TLR4 expression . 3, PPA intervention can inhibit allergen -induced HBECs Act1 expression . 4, PPA intervention can upregulate the expression of HBECs A20 to inhibit NF-κB pathway induced inflammation.

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