|
Objective: To study the Henan Han population different VKORC1-1639 G / A phenotype with warfarin maintenance dose relationship. Explore the Henan Han population VKORC1-1639 G / A phenotype distribution and allele distribution and with other regions and other ethnic Han population distribution were compared. Considering gender, age, body mass index (MBI), international normalized ratio (INR) and VKORC1-1639 G / A polymorphism in the gene dosage effect on China Falin Wei held to establish multiple regression equation guide individual treatment. METHODS: Screening warfarin patients, collecting its General information: ethnicity, gender, age, height, weight, disease, warfarin time, taking warfarin dose. Select one taken by the Shanghai Xinyi Pharmaceutical production warfarin (2.5mg / piece) of the patients, selected subjects were taking a stable dose of warfarin and whose INR has been reached (1.8-3.0), a total of 112 cases. Was extracted from peripheral blood 2ml, extracted DNA. Detection of patients with VKORC1-1639 G / A genotype. Statistical study of various phenotypes, calculate the frequency distribution, calculate allele frequencies. The results of this study and many overseas ethnic VKORC1-1639G / A polymorphism were compared. Compare different VKORC1-1639 G / A phenotype between warfarin maintenance dose differences. According to the study object's height and weight to calculate the body mass index, combined with the patient VKORC1-1639 G / A polymorphism and their gender, age, international normalized ratio, analyze the factors described above maintenance dose of warfarin effect, the use of Multiple linear regression equation established. Results: 112 patients, VKORC1-1639 G / A polymorphism distribution: AA genotype 93 cases (83.00%), GA genotype 18 patients (16.10%), GG genotype 1 case (0.90% ). A allele frequency of 204 (91.07%), G allele frequency distribution of 20 (8.93%). The study population VKORC1-1639 G / A polymorphism in Han population compared with other regions, the distribution of phenotypes and allele showed no significant difference, and other countries or ethnic phenotype distribution and other There were significant differences in allele (p <0.01). Different VKORC1-1639 G / A phenotype warfarin maintenance dose between different, AA genotype (2.44 ± 0.45) mg / day, AG genotype and GG genotype in patients (4.67 ± 0.89) mg / day, this difference statistically significant (p <0.01). Combined VKORC1-1639 G / A polymorphism, taking into account gender, age, INR and BMI China Falin Wei holding dose effects, the use of multiple regression equation obtained: y = -0.719 2.127 (VKORC1-1639 G / A gene phenotype) 0.632 (INR) a 0.11 (gender) -0.256 (age, years) 0.29 (RMI, Kg/m2) (r = 0.909, r2 = 0.826, p <0.01). Conclusions: 1 Henan Han population VKORC1-1639 G / A gene polymorphism obvious, AA type-based, rare GG genotype. 2, Henan Han population VKORC1-1639 G / A polymorphism in Han population distribution and other areas showed no significant difference compared with other ethnic groups was significantly different, further confirms VKORC1-1639G / A polymorphism with warfarin-related differences between nationalities. 3, AA genotype had lower daily warfarin maintenance dose AG genotype and GG genotype of patients. 4, the results of multiple regression equation VKORC1-1639 G / A polymorphism, gender, age, INR and warfarin maintenance dose of BMI are important factors.
|