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The molecular mechanisms of lung injury is still very imperfect, especially field induced lung injury in pulmonary fibrosis and immune complexes, and a lot of research work to be carried out. Small RNA (miRNA) average 22-nucleotide single-stranded, non-coding RNA. Currently miRNA is a very important body regulating factor, the mature miRNA target mRNA 3 'non-compilation area or compile District, thus silencing the target gene, and in many pathophysiological process plays a very important biological regulatory role. In order to study the role of miRNA regulation in pulmonary fibrosis, we extracted bleomycin induced pulmonary fibrosis in a mouse model of each time point of total lung RNA, miRNA microarray containing 1810 probe miRNA high-throughput detection, Compare bleomycin different time points and the control group in total lung RNA differentially expressed miRNAs. The research was supported by the 161 differentially expressed miRNA with the normal group. Analysis of differentially expressed miRNAs in lung fibrosis model early and late in the pathological process. Application of bioinformatics methods, the target prediction analysis of miRNAs differentially expressed in different pathological processes. These important miRNA let-7f, let-7g, miR-127, miR-196b, miR-16, miR-195, miR-25, miR-144, miR-351, miR-153, miR-468, miR -449b, miR-361, miR-700, miR-704, miR-717, miR-10a, miR-211, miR-34a, miR-367, and miR-21. prediction of miRNA targets to be classified analysis, been an important pathological aspects of pulmonary fibrosis, including apoptosis, inflammation and fibrosis important molecular pathways, such as apoptosis signaling pathway, Wnt signaling pathway, Toll receptors and tissue growth factor pathway closely related miRNA and its target point gene. Our study reveals miRNAs differentially expressed in varying degrees in the process of pulmonary fibrosis predicted miRNA may regulate pulmonary fibrosis pathological processes important molecular pathways. In addition, we found that the downregulation of miR-127 in the inflammatory response of the lung damage stage. Based on this, we explore the mechanism of action of miR-127 in lung injury and inflammation. We found that over-expression of miR-127 of the macrophage cell inflammatory stimuli role in the production of cytokines [White mediated prime -6 (IL-6), White mediated prime 1p (IL-1β), organizations necrosis factor (TNF-α)] release reduced. Bioinformatic analysis may be found immunoglobulin G (IgG) Fcγ receptor I (FcyRI/CD64) of miR-127 target genes. Gene chip, protein quantitative expression analysis of miR-127 were confirmed at the mRNA and protein levels of CD64 regulation. By constructing a fluorescent reporter plasmid containing CD64-3'UTR, we identified miR-127 CD64 directly target relationship. In addition, in vivo experiments further found that intranasal instillation miR-127 can be reduced IgG immune complexes (IgG IC) as a result of the acute the intrapulmonary damage and inflammatory response. Including the lung tissue morphology observed, cytokines IL-6, TNF-a, macrophage inflammatory protein-1β (MIP-1β), macrophage inflammatory protein -2 (MIP-2) monocyte chemoattractant protein ( MCP-1), chemotactic factor CXC ligand -1 (KC), detection, detection of lung permeability, complement C5a and the acute phase response factor signal transducer and activator of transcription 3 (STAT3) protein levels. Therefore, we think that miR-127 macrophage CD64 expression by the target, thereby reducing the inflammatory response induced by the intrapulmonary IgG IC. In summary, we have studied the expression of miRNAs in specific point in time and specific pathological stage differences in pulmonary fibrosis, and these miRNA target gene prediction and corresponding molecular signaling pathways of these target genes with specific pathological stage, we analyze various pathological stage miRNA may play a role in the molecular signaling pathway. Through the study of the mechanism of action of the inflammatory response in miR-127, we determine the role of miR-127 target genes of CD64, and found that the protective effect of miR-127 acute lung injury induced IgG IC. Intrapulmonary fibrosis and inflammatory reaction mechanisms of miRNAs regulating miRNA research reveals an important role in the regulation and control of infectious and non-infectious inflammatory response of the lung injury, provide a strong basis for future drug research of pulmonary fibrosis and lung injury inflammatory response . This study is the role of miRNA in lung injury and repair mechanisms. The first part of the study: bleomycin induced pulmonary fibrosis in miRNA complexity analysis reveals the many ways the study of molecular mechanisms, writing papers have been published in Physiological Genomics (Impact Factor: 3.931) magazine. The second part of the study: miR-127 regulation mechanism of the inflammatory response in the lungs, Journal of Immunology (Impact Factor: 6.0) magazine receiver is expected to be published in nearly half of the year. The content on this study, the research focus at home and abroad, the research methodology is a leading domestic and international level. Chinese medicine is an indispensable part of the World Medical, a place in the traditional Chinese civilization is the cultural treasures of the Chinese People's intergenerational, has to play a major role for the treatment of many diseases. This study can be a high level of high-quality research platform to build for the future in pharmaceutical research, provides state-of-the-art research methods and techniques for the study of Chinese medicine to treat lung disease.
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