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Expression of CLEC-2BmRNA and the Relationship with the Clinical of Vitiligo

Author: YuWang
Tutor: ZhangJunLing
School: Tianjin Medical University
Course: Dermatology and Venereology
Keywords: Vitiligo CLEC-2BmRNA Genotyping Installments Outcome Correlation Protein expression
CLC: R758.41
Type: Master's thesis
Year: 2011
Downloads: 33
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Abstract


Objective To investigate the CLEC-2B (C-type lectin domain family 2, member B) gene expression in peripheral blood of patients with vitiligo skin lesions and disease clinical types, duration, stage of disease, outcomes, and the lesion area CLEC-2B protein gene expression, cellular localization of the clear the CLEC-2B gene expression. Through this experiment found that the diversity of disease and clinical outcomes with differential gene expression to predict clinical classification, staging and prognosis of vitiligo occurs, clinical played a guiding role. The application of reverse transcription - polymerase chain reaction (reverse transcription-polymerase chain reaction, RT-PCR) method for analysis of skin tissue and peripheral blood of vitiligo patients and normal controls expression of CLEC-2B, amplification products confirmed by 4.0% agarose ethidium bromide electrophoresis gel imager camera. Using statistical software to analyze the different clinical forms, according to the diagnostic criteria of vitiligo, a different stage of disease, different course, a different outcome vitiligo lesion area, peripheral blood and normal skin, peripheral blood the CLEC-2B gene expression differences. And patients with vitiligo lesional tissue and normal skin tissue by immunohistochemical methods to determine the CLEC-2B protein gene expression in the skin tissue. 1 skin lesions of patients with vitiligo group the CLEC-2BmRNA expression level is higher than the normal control group, the difference was statistically significant (t = 2.581, P lt; 0.05). The 2. Peripheral blood of vitiligo patients and normal controls expression of CLEC-2BmRNA difference was not statistically significant (t = 1.375, P gt; 0.05). CLEC-2BmRNA expression levels. The different gender vitiligo skin lesions of patients with group showed no statistical difference (t = 1.341, P gt; 0.05). 4. Different course of vitiligo skin lesions of patients of CLEC-2BmRNA the expression is no statistically significant difference (F = 1.479, P gt; 0.05). 5. The CLEC-2BmRNA expression vitiligo vulgaris lesions group than segmental vitiligo group, the difference was statistically significant (t = 2.432, P lt; 0.05). 6 vitiligo patient group with advanced lesions the CLEC-2BmRNA expression amount higher than patients with stable group, the difference was statistically significant (t = 2.204, P lt; 0.05). Vitiligo patients in different outcome groups lesions CLEC-2B mRNA expression of the amount of the difference was statistically significant (F = 5.218, P lt; 0.05). 8 confirmed by immunohistochemical methods CLEC-2B protein expression in lymphocytes in vitiligo skin dermal layer. Conclusion 1. Patients with vitiligo lesions of CLEC-2B expression levels increased, suggesting that CLEC-2B has a certain significance in the pathogenesis of vitiligo. 2 the vitiligo patients progress CLEC-2B, expression levels than patients with stable, suggesting that CLEC-2B vitiligo patients progress. 3. CLEC-2B expression levels in vitiligo vulgaris than segmental vitiligo, suggesting that CLEC-2B to play a role in the decision of vitiligo typing in, and speculated CLEC-2B immune mechanisms may be involved in vitiligo. 4. Significant differences in expression levels of different outcome vitiligo patients CLEC-2B, which reveal a different outcome of the same disease at the genetic level the answers, to guide clinical diagnosis and prognosis. The foundation at the same time looking for drugs, regulation of the gene to improve disease outcome. 5. CLEC-2B protein expression in the nucleus of lymphocytes in the dermis layer of skin lesions in patients with vitiligo, further deepen the understanding of the gene.

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CLC: > Medicine, health > Dermatology and Venereology > Dermatology > Metabolic disorders of the skin > Vitiligo ( vitiligo )
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