Dissertation > Excellent graduate degree dissertation topics show

Changes of Periodontal Ruffini Endings and Expression of Related Proteins in the Opposite Molar of the Orthodontically Moved Molar in Rats

Author: LuLei
Tutor: WangMeiQing；LiuXiaoDong
School: Fourth Military Medical University
Course: Stomatology
Keywords: Periodontal Ruffini nerve endings Orthodontic Tooth Movement Occlusal Schwann cells NT-3
CLC: R783.5
Type: Master's thesis
Year: 2011
Downloads: 41
Quote: 0
Read: Download Dissertation

Abstract


The masticatory system masticatory muscles, the temporomandibular joint and bite under the control of the nervous system functions as a whole, any exception occurs will lead to other parts of the alteration or dysfunction. Periodontal ligament force receptors - periodontal Ruffini nerve endings responsible for feeling the bite force and force stimulation, and will force through the afferent stimulation of the a signal incoming central nervous system, coordinated chewing movement. Occlusal contact female Sprague-Dawley (SD) rats, to orthodontic apron expansion pushing the left maxillary and right mandibular third molar to distal movement formation on mandibular molars tip nest does not match for the orthodontic tooth movement in the rat model of orthodontic tooth movement jaw periodontal Ruffini nerve endings of microscopic, ultrastructural changes of Schwann cell marker S-100 protein expression, neurotrophin-3 ( Neurotrophin-3, NT-3) expression changes. Part I: 24 8-week-old female SD rats molars were randomly divided into orthodontic tooth movement jaw teeth, orthodontic tooth movement group and the control group, which orthodontic tooth movement jaw teeth (the right maxillary third molars) for the experimental group, orthodontic tooth movement group (the left maxillary third molar) as a positive control group of orthodontic tooth movement of the jaw teeth group in the same animals. Implementation of orthodontic tooth movement after three days, 14 days and 28 days (n = 4), the bilateral maxillary posterior teeth do hematoxylin - eosin (with hematoxylin-eosin, HE) staining, microscopic observation of the left maxillary first three molars and right maxillary third molar periodontal tissue microstructure changes. The rat maxillary third molar 3 root order mesiobuccal root, near the base of the tongue and the distal root for uniform standards, whichever is more coarse and far observed in the root. The results show that the blank control group, bilateral maxillary third molar distal root of nearly uniform width of the distal side of the periodontal ligament cavity, no significant periodontal ligament compression and tension; 3 days after orthodontic tooth movement, orthodontic mesial tooth movement (left maxillary third molar) periodontal membranes cavity significantly narrows significantly wider distal periodontal ligament cavity, near periodontal ligament compression far in the periodontal ligament is stretched, showed that orthodontic mobile teeth apical to the mesial movement; 14 days after orthodontic tooth movement, orthodontic tooth movement nearly periodontal ligament cavity mild narrow jaw teeth (on the right maxillary third molar) in the distal periodontal ligament mild compression cavity, near periodontal tissue distal periodontal tissue by mild stretching that orthodontic tooth movement of the maxillary teeth there apical mild mesial movement; orthodontic tooth movement after 28 days, periodontal ligament width of orthodontic tooth movement and orthodontic tooth movement of the maxillary teeth to restore uniformity. Conclusion: Orthodontic tooth movement will cause the jaw teeth, periodontal tissue remodeling, the time, the extent of the alterations appear more orthodontic tooth movement occurs late, light, quick recovery. Part II: 64 8-week-old female SD rats were divided into two batches, the first batch of 16 is used electron microscopy for microscopic observation, the second batch of 48, each batch were randomly divided into orthodontic tooth movement on group of jaw teeth, orthodontic tooth movement (positive control) and blank control group. Adaptability keeping the first batch of blank control group at 3 days after the orthodontic tooth movement group of jaw teeth and orthodontic tooth movement in orthodontic tooth movement after three days, 14 days, 28 days (n = 4), we take the side maxillary third molar distal root apical 1/3 periodontal tissues do electron microscopy. Second batch in orthodontic tooth movement after 1 day, 3 days, 7 days, 14 days, 21 days and 28 days (n = 4), the bilateral maxillary posterior teeth do the periodontal Ruffini nerve endings marker protein gene product 9.5 (protein gene product 9.5, PGP 9.5) immunofluorescence staining. More normal ultrastructure in the blank control group, the periodontal Ruffini nerve endings, mitochondria, microfilaments, microtubules and nerve basement membrane structure typical immunofluorescence PGP 9.5 positive signals were mainly distributed in the cavity of the periodontal ligament alveolar bone side; orthodontic tooth movement group, periodontal Ruffini nerve endings ultrastructure temporary mitochondria reduce and swelling, vacuole-like structures, amorphous electron-dense material, microfilaments microtubules arranged in disorder, nerve base the film arranged disorderly injury like changes, change in three days after orthodontic tooth movement the most obvious Immunofluorescence showed PGP 9.5 positive signals appeared in the side of the middle belt and the root of the periodontal ligament, PGP 9.5-positive area percentage compared with the control group temporary reduction, the lowest value in the experiment of the first three days; orthodontic tooth movement of the group of jaw teeth, three days after the orthodontic tooth movement, periodontal Ruffini nerve endings appear a transient mitochondrial centrifugal arranged, orthodontic tooth movement 14 days, periodontal Ruffini nerve endings to reduce mitochondrial microfilaments the sparse and arrangement of microtubules disorders and nerve basement membrane structural abnormalities immunofluorescence PGP 9.5 positive signals in the intermediate zone and the root side of the periodontal ligament, PGP 9.5-positive The area percentage compared with the control group the temporary reduction in the lowest value in the 14 days of the experiment; 28 days to experimental orthodontic movement of teeth and jaw teeth ultrastructure and PGP 9.5-positive area percentage change basic recovery. Conclusion: Orthodontic tooth movement can cause the jaw periodontal Ruffini nerve endings in the ultrastructure, number and distribution of temporary changes in orthodontic tooth movement, the extent of the change, rapid recovery. Part III: 48-week-old female SD rats were randomly divided into orthodontic tooth movement of the group of jaw teeth, orthodontic tooth movement (positive control) and blank control group, the implementation of one day after orthodontic tooth movement, 3 days, 7 days, 14 days, 21 days and 28 days after bilateral maxillary teeth out do Schwann cell marker S-100 and the neurotrophic factor NT-3 immunofluorescence staining and colocalization observed and found that orthodontic movement dental group S-100 positive area percentage, a temporary increase in relative optical density of NT-3 compared with the control group, is widely expressed in the periodontal membrane chamber, the highest value in the experiment of the first three days; orthodontic tooth movement of the maxillary teeth positive area percentage of S-100 and NT-3 relative optical density than the control group also increased temporary widely co-expressed, but the highest value in the experiment of the first 14 days. Orthodontic tooth movement and orthodontic tooth movement through the percentage of positive area maxillary teeth S-100 and NT-3 relative optical density in 28 days and returned to baseline levels. Conclusion: Orthodontic tooth movement will cause a temporary increase in Schwann cell structure of the maxillary teeth and NT-3 expression, but the change than orthodontic tooth movement occurs late and quick recovery. Orthodontic tooth movement operation allows tooth movement of the maxillary teeth appear similar to the same mobile dental periodontal membrane cavity change, accompanied by similar periodontal Ruffini nerve endings can be obtained through the above study, the following conclusions: alterations, the marker protein gene product 9.5 expression and teeth Zhou Xuewang cell marker substance S-100 and NT-3 expression changes. Due to orthodontic movement on the mandibular cusp fossa consistent occlusal contacts related to bite force, this periodontal tissue, the force receptors Ruffini nerve endings, and Schwann cells remodeled the internal mechanism.

Related Dissertations

  1. A Simple and Efficient Method That Uses Low Concentration Fetal Bovine Serum to Culture and Purify Schwann Cells,R329
  2. The Preliminary Study of Growth-differentiation Factor-15 in Heart Failure Patients,R541.6
  3. Evaluation of between the Occlusal Force and the Tissues Health Surrounding Implant of ITI Implants with Zirconia All-ceramic Crown,R783
  4. Effects of NF-кB Signaling Pathways on Cyclic Stretch―Induced Human Periodontal Ligament Fibroblasts Proliferation and Its Mechanism,R781.4
  5. bFGF chitosan microspheres and promote Schwann cell proliferation studies,R651.2
  6. Technology research and analysis of industrial process data correction,TP273
  7. Application Research of the Evaluation of Occlusal Contacts in the Orthognathic Surgery Based on ABAQUS,R782
  8. Study of sEMG Variation of Masticatory Muscles Before and after Restoration in Patients Who Lost the Occlusal Relationship,R783
  9. The Relationship between Vertical Dimension and Bite Force, Electromyography in Edentulus People,R783.6
  10. Researching the Shape of Normal Occlusion Dental Arch and Base Bone Arch of College Students of Han Nationality in Liaoning.,R783.5
  11. Clinical Analysis above the Changes of the Occlusion Through Rigid Internal Fixation after the Mandibular Osteotomy of Tongue Carcinoma,R739.86
  12. Three-dimensional Finite Element Analysis of Rapid Canine Tooth Movement in Periodontal Ligament Distraction,R783.5
  13. The Application of Ultrasonographic Examination of Fetal Nuchal Translucency, Nasal Bone, Which Combined Measurement Maternal Serum Free Beta-HCG, and Pregnancy-associated Plasma Protein a for Screening Down’s Syndrome in the First-trimester.,R714.5
  14. Study of Subregional Tissue-engineered Conduits Addition with NT-3 on Repair of T8 Complete Transection Injury in Rat Spinal Cord,R651.2
  15. Clinical Characters and Imaging Features of Left Ventricular Noncompaction in Chinese,R541.1
  16. The Expression and Effect of CDK11p58 in Schwann Cells under Inflammation,R363
  17. The impact of exercise training on diabetic neuropathy in rats,R587.1
  18. The Effect of SSeCKS in Schwann Cells under Inflammation,R363
  19. Expression and Significance of ICAM-1 in Inflammation of Nervous System,R392
  20. The Study about the Recovery of Nerves Function in Rats of Spinal Cord Injury by Step Training,G804.7
  21. Mandibular Vectorial Cephalometric Analysis in North China People,R783.5

CLC: > Medicine, health > Oral Sciences > Oral orthotics > Journal of Orthodontics
© 2012 www.DissertationTopic.Net  Mobile