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The Study on RA Signal Pathway in RPE-choroid of FDM Guinea Pigs

Author: ZengLiMing
Tutor: ZhaoShaoZhen
School: Tianjin Medical University
Course: Ophthalmology
Keywords: Form deprivation myopia Retinal pigment epithelium - choroid Transforming growth factor β2 Activator protein-1 Curcumin LE540
CLC: R778.11
Type: Master's thesis
Year: 2011
Downloads: 20
Quote: 0
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Abstract


The purpose of the establishment of the early form deprivation myopia (form-deprivation myopia, FDM) animal model, detection of guinea pig retinal pigment epithelium - choroid of transforming growth factor β2 (transforming growth factor-beta2, TGF-β2) and activated protein -1 (activator protein-1, AP-1) level; shaped deprivation at the same time were given a specific inhibitor of AP-1 inhibitor curcumin and retinoic acid receptor β (retinoic acid receptorβ, RARβ) LE540 compare different treatment of flexor light state, and the detection of the retinal pigment epithelium - choroid TGF-β2 and AP-1 levels to explore TGF-β2 and AP-1 form deprivation myopia in guinea pig retinal pigment epithelium - choroid and, as sulfonic acid (retinoic acid, RA) the relationship of the signal transmission process. The first part of the method: the same batch of 3-week-old guinea pigs, 12, wearing a wig left eye covered, right eye as its own control eyes. Reared to 21 days as a form deprivation in animal models. Observations: 1. Were measured before and after the experiment refraction, the removal of the eye after the end of the experiment, measuring the axial length; 2. Measured by ELISA retinal pigment epithelium - choroid content of TGF-β2, western blotting detection of retinal pigment epithelium - choroid AP 1 content of the change. Part II: elect the same kind of guinea pig 40, were randomly divided into four groups group I, 6, 12 each for the normal control group, Ⅱ, Ⅲ, Ⅳ group. Ⅱ group for experimental control group, the left eye after injection 0.1% DMSO0.1ml; 0.1m (1.5g / L) of curcumin Ⅲ group left eye after injection; N left eye after injection 0.1ml (0.1 g / L) LE540; Ⅱ, Ⅲ, Ⅳ group every other day administered once, a total of three times to cover the left eye to 21 days. Right eye to control eye itself. Observations with the first part. Measurement data x ± s, using SPSS16.0 statistical software for statistical analysis, and the variance of the normal distribution and Levene test of homogeneity underwent t-test and variance analysis. The results of the first part: 12 animal experiments eye induced relative myopia and self-control eye difference was statistically significant (t = -0.575, P = 0.001). The experimental eye axial length longer than its own control eyes (t = -4.920, P = 0.000). The experimental retinal pigment epithelium - choroid in the level of TGF-β2 significantly lower compared with its own control eyes (t = -4.933, P = 0.004), Western blotting proceeds of c-Fos and GAPDH grayscale display, the experimental retinal pigment epithelium - choroid AP-1 activity compared with its own control eyes to reduce (t = -2.832, P = 0.037). Part II: experimental intervention Ⅱ, Ⅲ and group Ⅳ experimental eye formation relative myopia about the three groups the diopter difference was statistically significant (P lt; 0.05). Between group Ⅲ and Ⅳ group before and after experiment experimental eye diopters difference was statistically significant (P lt; 0.05). Group Ⅱ, Ⅲ and Ⅳ group after the intervention of the left and right eye axial length differences were statistically significant (P lt; 0.05). Group Ⅱ, group Ⅲ and group Ⅳ experimental retinal pigment epithelium - choroid in TGF-β2 content control eyes was significantly lower (P lt; 0.05) compared with itself; experiment I the eyes retinal pigment epithelium - choroid TGF-β2 content self-control eyes compared to the difference was not statistically significant (P gt; 0.05). Group Ⅱ, Ⅲ and Ⅳ group after the experiment compared to the the experimental retinal pigment epithelium - choroid in AP-1 activity expression and self-control eyes were significantly lower (P lt; 0.05); experiment I the eyes retinal pigment epithelium - choroid in AP-1 activity expression difference was not statistically significant (P gt; 0.05) compared with its own control eyes. The conclusions form deprivation can lead to myopia; guinea pigs early form deprivation retinal pigment epithelium - choroid AP-1 activity and TGF-β2 downregulation; AP-1 inhibitor curcumin can affect guinea pig form deprivation myopia the role of the formation, guinea pigs early form sense of deprivation of the retinal pigment epithelium - choroid TGF-β2 expression by the AP-1 activity; of retinoic acid receptor β was inhibitor LE540 use can affect the formation of the guinea pig early form deprivation myopia role; guinea pigs early form deprivation retinal pigment epithelium - choroid RARβ can regulate the activity of AP-1 and TGF-β2 expression of AP-1 and TGF-β2 may be an important factor of the RA signaling pathway regulation of guinea pig FDM formed, RA - RARp - AP-1 - TGF-beta2 visually sulfonic signal pathway may be formed in one of the important pathways regulating guinea FDM.

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CLC: > Medicine, health > Ophthalmology > Eye dioptrics > Eye application dioptrics > Myopia
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