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The purpose of rat cerebral hemorrhage (CH) induced multiple organ dysfunction syndrome (MODS) pathological changes of the model were observed after the lung, liver, kidney and small intestine; immunohistochemical assay hypothalamus FOS protein expression and medullary visceral zone (MVZ ) the expression of choline acetyltransferase (CHAT). Explore the role of brain hemorrhage caused by multiple organ dysfunction syndrome rat model of the hypothalamus, the medullary visceral zone and fans vagus nerve; reveal cerebrogenic multiple organ dysfunction syndrome (CMODS) possible regulatory mechanism of the hypothalamic, medullary visceral zone and vagus nerve. Method 1 of Wistar rats 80 were randomly divided into: sham surgery group (10 rats), vagus nerve cut (SDV) group (10 rats), cerebral hemorrhage (CH) group (20 cases), cerebral hemorrhage fans walk nerve cut (CH SDV) group (20), the the cerebral hemorrhage vagus nerve stimulation (CH SIV) group (20). 2. Caudate nucleus collagenase 0.8 U and 3.5 IU heparin sodium the rat CH model mixture injection method; vagotomy (SDV) group rats were anesthetized and underwent laparotomy, at the lesser curvature of the stomach exposed vagus nerve around two Ganzhi about 5 mm at the distance of each branch of the vagus nerve the ligature around two Ganzhi and cut off. Vagus nerve stimulation (SIV) surgery rats exposed to bilateral cervical vagus nerve; handles, silver electrode made of ferrule ferrule handle the outside brush insulating paint rings inside of the electrode surface, the ferrule will hold back the vagus nerve, irrigation The insulation and the surrounding tissue into the paraffin oil from the back of the neck, piercing the skin incision was sutured. 3. Records rat brain hemorrhage postoperative body temperature, heart rate, breathing and other symptoms and signs of change at each time point, detect blood count, liver, renal and cardiac enzymes change the light microscope after intracerebral hemorrhage in rats 24 hours time points, lung, liver, kidney, small intestine organ tissue pathological changes and camera, according to the diagnostic criteria to determine the incidence of SIRS, MODS. Using immunohistochemistry ABC method observed hypothalamus FOS protein expression. 5. Immunohistochemical assay medullary visceral zone acetylcholine transferase (CHAT) expression. The the results 1.CH group CH SDV group and CH SIV rats occurrence of SIRS; the CH group meet the MODS rats 70.0% (14); MODS rats CH SDV group, 75.0% (15); CH SIV group meet the MODS rats 65.0% (13). Sham group and SDV liver, renal and cardiac enzymes was no significant difference in the degree of abnormality, liver, kidney, lung, intestinal tissue pathological damage light. Group of CH SDV, CH SIV group and CH group of liver and kidney function, degree of enzyme abnormalities and liver, kidney, lung, intestinal pathological damage are more important than the sham group and SDV group CH the SDV group of heavy CH group, while CH SIV group was significantly lighter than the CH group. 3. CH SDV group rat hypothalamus in FOS protein expression was significantly more than the sham group (9.3 ± 2.6 and 2.5 ± 0.4, t = 8.17, P lt; 0.01) and SDV group (9.3 ± 2.6 and 2.9 ± 0.5, t = 7.64, P lt; 0.01), but significantly less than the CH group (9.3 ± 2.6 and 48.6 ± 18.1, t = 6.80, P lt; 0.01), the CH group was significantly less than the CH SIV group (48.6 ± 18.1 and 74.25 ± 6.29, t = 4.23, P lt; 0.01) 4 CH SDV group MVZ CHAT positive expression was significantly more than the sham group (138.49 ± 11.76 and 108.15 ± 9.54, t = 5.39, P lt; 0.01) and SDV group (138.49 ± 11.76 and 110.71 ± 9.88, t = 4.83, P lt; 0.01), but significantly less than the CH group (138.49 ± 11.76 and 164.42 ± 12.44, t = 3.53, P lt; 0.01), the CH group was significantly less than the CH SIV group (164.42 ± 12.44 and 189.38 ± 14.97, t = 3.14, P lt; 0.05). Conclusion 1. Hypothalamus - the medullary visceral belt - brain-derived features multiple organ dysfunction syndrome vagus nerve pathway mechanism; 2. Vagus nerve may be a brain hemorrhage caused by multiple organ dysfunction syndrome bidirectional regulatory pathways.
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