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The first part of the cerebral ischemia at different times nerve function and infarct size comparison purposes: To investigate Longa intraluminal rat cerebral artery ischemia-reperfusion (modified neurological severity scores MCAO) model of infarction and neurological deficits caused by Stability at different times; explore the line intraluminal die ischemia reperfusion neurological function and infarct size, MCAO model in order to clear nylon monofilament prepared reliability and best ischemia time, provide the basis for further experiments. Methods: 22 SD rats were randomly divided into 1h (n = 6), 2h (n = 6), 4h (n = 6) and 6h (n = 4) group, referring to Longa intraluminal MCAO model, respectively, in the ischemia 1h, 2h, 4h and 6h reperfusion after molding 1d, 3d, 5d, 7d rats improved neurologic deficit score (modified neurological severity scores, mNSS), the 7th day after the rats were sacrificed and the brain was conducted The TTC staining calculation infarct size. Results: MCAO rats after nerve function defect obvious score which MCAO s Id mNSS, 1h, 2h, 4h and 6h group were 6.33 ± 0.82,9.83 ± 1.17,10.83 ± 1.17,12.00 ± 0.81, which, inter 2h 4h group, 4h and 6h group difference was not statistically significant (P gt; 0.05), other groups were statistically significant (P lt; 0.05); molding rats after 7d survival rates were 66.7% (6/9), 46.2 % (6/13), 35.3% (6/17), 16.0% (4/25), at 1h, 2h and 6h group survival rate differences were statistically significant (P lt; 0.05), among other groups the difference was not statistically significant (P gt; 0.05). The percentage of infarct size four groups (.1139 ± 0.0122)%, respectively (.2279 ± 0.0309)% (0.2509 ± 0.0229)% (0.2839 ± 0.0220)%. 1h group of primary cortical infarction, 2h, 4h, 6h infarction cortex and the basal ganglia. In addition 2h 4h group was significantly less than 6h group (P lt; 0.05), the remaining difference between the groups was not statistically significant (P gt; 0.05). Conclusion: 1. Prepared the Longa line occlusion of cerebral ischemia model best ischemia reperfusion time was 2h; Longa line occlusion of the rat cerebral ischemia model is simple and stable, controllable, repeatable good . After the second part of the rat MCAO chronic phase GFAP, NSE, SYN, Nogo-A expression and nerve function outcome correlation Objective: To investigate the middle cerebral artery ischemia-reperfusion convalescent recovery of neurological function and glial fibrillary acidic protein (glial fibrillary acidic protein, GFAP), neuron-specific enolase (neuron-specific enolase, NSE), synaptophysin (synaptophysin, SYN), axonal overgrowth inhibitory factor (Neurite outgrowth inhibitor-A, Nogo-A relationship) expression. Methods: 24 SD rats were randomly divided into 28d, 35d, 42d and 49d group, using Longa suture method ischemia 2h reperfusion of cerebral ischemia model, respectively, in the 28d, 35d, 42d, 49d rats improved nerve deficit score (modified neurological severity scores, mNSS), and observed by immunohistochemical brain tissue of GFAP, NSE, SYN, Nogo-A expression. Results: rat MCAO reperfusion over time, mNSS score decreased addition to the 35d group (5.11 ± 0.737) and 42d group (4.54 ± 0.519), 42d group and 49d group (4.29 ± 0.488) unrestricted differences among other groups were statistically significant (P lt; 0.05). Group number of GFAP-positive cells from 28d to 49d group gradually reduce the 42d group (51.00 ± 13.59) and 49d group (44.38 ± 11.94) was significantly less than the 28d group (69.00 ± 15.10) (P lt; 0.05); Although NSE positive cells the number of differences between groups was not statistically significant, but NSE accumulated optical density (integrated optical density, IOD) gradually increased, 28d group (6218.57 ± 1864.25) and 42d group (9414.00 ± 2491.12), 49d group (12522.50 ± 3106.99), 35d group (7343.40 ± 1533.35) and 49d group differences significant with (P lt; 0.05), among other groups without significantly with differences (P gt; 0.05); SYN expression IOD gradually increased, 49d group (66503.00 ± 12834.61) significantly with higher than 28d group (43905.14 ± 13208.59) (P lt; 0.05), Nogo-A positive cells gradually reduce the number of 49d group (42.13 ± 14.45) was significantly less than the 28d group (59.57 ± 15.25) (P lt; 0.05). The GFAP expression mNSS score was positively correlated (r = -0.992, P = 0.008), and the NSE (i = -0.954, P = 0.044), SYN (r = -0.992, P = 0.008) expression mNSS score was negatively correlated. Conclusion: rat MCAO reperfusion convalescent recovery of neurological function and GFAP expression decreased, NSE and SYN expression was associated with increased; 2. Proliferation of reactive astrocytes cerebral infarction may be where disease different stages instead of one.
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