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Study on the Polymorphism in CC10 Gene of Wheezing Children under Five Years Old

Author: HouYingYing
Tutor: WangXiuFang
School: Zhengzhou University
Course: Pediatrics
Keywords: Child Respite Clara cell secretory protein 10 Gene polymorphism
CLC: R725.6
Type: Master's thesis
Year: 2011
Downloads: 19
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Abstract

Wheezing are common symptoms of infancy, the majority of patients with asthma can be traced back episodes of wheezing before age 3. Learn wheezing children under 5 years the link between asthma is one of the biggest challenges, most studies suggest that such children with lower airway infection can cause early wheezing, some of these patients polymorphic loci with the subsequent immune response. Human Clara cell secretory protein 10 (CC10) gene polymorphism and asthma and atopy susceptibility in recent years about CC10 gene polymorphisms and atopic diseases and breathing disorders related research confirms, CC10 gene is asthma and atopic diseases is an important candidate genes. Objective This study of wheezing in children under 5 years old CC10 gene polymorphism study on the CC10 gene polymorphism with different clinical phenotypes of wheezing between. Materials and methods The experiment was divided into two groups wheezing, asthmatic group selected from September 2009 to April 2010 in our hospital pediatric clinics and wards were 120 cases of recurrent wheezing in children, both acute phase, including 31 females , male 89 cases, aged 6 months to 4 years and 3 months, the average age of a year old in October. Children in the past 12 months, wheezing, or at least three times over the past six months at least two times a wheezing, exclusion or small for gestational age preterm children and children who have congenital diseases. Ask family history carefully selected cases and allergies, the group was divided into asthmatic wheezing and wheezing group Ⅰ Ⅱ group, according to one of the parents or the parents of children who have a history of asthma or allergic disease itself (including eczema, allergic dermatitis, allergic rhinitis) as atopic risk factors, patients with risk factors by wheezing group Ⅰ, no high risk factors for the wheezing group Ⅱ. Including wheezing group Ⅰ 67 cases (45 cases of eczema, allergic dermatitis four cases, five cases of allergic rhinitis, parents or one parent has a history of asthma 13 cases), male 46 cases, female 21 cases, wheezing group Ⅱ 53 cases, male 38 cases, female 15 cases. Select the control group over the same period in our hospital surgical recent history of non-infectious diseases in children 55 cases, 31 males and 24 females, aged 11 months to 4 years, 5 months, with an average age of 2 years and 8 months, were excluded from atopic risk factors, no history of infectious diseases. Three groups of children were born at term, most recently two weeks without systemic or topical steroid use, no other immunomodulatory agents use history between the two groups were not statistically different ages, have received parental consent. In this study, PCR-restriction fragment length polymorphism on 120 cases of wheezing in children under 5 years old, and 55 cases in the control group children CC10 gene G38A locus detection method using ELASA serum CC10, total IgE levels were detected, while computing peripheral blood eosinophil count. Statistical analysis All data were statistically analyzed using SPSS16.0 software. Direct calculation of allele frequencies in each group, the differences for the genotype distribution fourfold table using chi-square test, when n ≥ 40 and theoretical frequency (T) ≥ 5 time by ordinary chi-square test; when n ≥ 40 but a ≤ T lt; 5 time by corrected chi-square test; when n lt; 40 or T lt; 1 time by Fisher's exact test. Genotypes and the serum CC10 concentrations between groups, IgE levels and eosinophil count using single factor analysis of variance, pairwise comparisons using LSD-t method with P lt; 0.05 considered statistically significant. Results 1. Asthmatic group and control group genotype and allele frequency differences between them CC10 gene G38A genotype frequencies: AA0.20, GA0.44, GG0.34, compared with the control group, the difference was significant (P lt; 0.05); wheezing group Ⅱ CC10 gene G38A genotype frequencies: AA0.09, GA0.32, GG0.58, compared with the control group showed no significant difference (P gt; 0.05); wheezing Ⅰ and group Ⅱ wheezing CC10 gene G38A genotype frequencies were significant difference compared (P lt; 0.05); wheezing group Ⅰ A allele frequency was 0.43, compared with control group, the difference was statistically significant (P both lt; 0.05); wheezing group Ⅱ A allele frequency was 0.26, compared with the control group showed no significant difference (P gt; 0.05); wheezing group Ⅰ and group Ⅱ wheezing compared to the frequency of A allele was significantly sex (P lt; 0.05). 2 wheezing and control groups CC10 G38A AA genotype CC10 concentration was significant difference compared (P lt; 0.05), wheezing group Ⅰ Ⅱ serum CC10 concentrations below wheezing group and the control group, the difference was significant ( both P lt; 0.05); wheezing Ⅱ group and the control group was not statistically significant compared CC10 concentration (P gt; 0.05). 3 wheezing and control groups CC10 G38A AA genotype counts of total IgE and peripheral blood EOS significant difference compared (P lt; 0.05), wheezing group Ⅰ total serum IgE and peripheral blood counts were higher than wheezing Ⅱ EOS group and the control group (P lt; 0.05), wheezing group Ⅱ total serum IgE and peripheral blood EOS counts compared with the control group was not statistically significant (P gt; 0.05). 4 wheezing group and control group, serum CC10 concentration, IgE and EOS counts were statistically significant (P lt; 0.05), compared with asthmatic wheezing group Ⅰ Ⅱ CC10 concentration and control groups, and the difference was statistically significant (P lt; 0.05); wheezing group Ⅰ than IgE and EOS counts wheezing Ⅱ group and control group, the difference was statistically significant (P lt; 0.05). Conclusion with atopic and carrying AA genotype and A allele wheezing in children under 5 years old the lowest concentration of serum CC10, and total serum IgE levels and peripheral blood EOS counts increased airway inflammation such children is to eosinophil cell counts represented allergic factors play a role in the occurrence of wheezing, asthma more likely to develop into the future.

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