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Research on Monitoring and Controlling Neural Circuits Basing on Femtosecond Laser and Random Access Scan

Author: LiuXiuLi
Tutor: ZengShaoQun
School: Huazhong University of Science and Technology
Course: Biomedical Engineering
Keywords: Neural circuits Random scan Femtosecond laser Probe Stimulate
CLC: R318.51
Type: PhD thesis
Year: 2009
Downloads: 84
Quote: 0
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Abstract


The advanced features of the brain is the result of the synergy of neural circuits, neural circuits and its principle of information processing in recent years, brain research hotspot. Although research has made some significant achievements, but the composition of the various cells of the neural circuits in the role of the slow progress of research in information processing. Existing micro-electrode array chip technology is difficult to determine the positioning of single neuron network, commercial multi-photon imaging system is not high time resolution, it is difficult to meet the rapid changes in nerve function signal detection needs, the activity of neural circuits control the lack of effective means . Based acousto-optic deflector and random femtosecond laser scanning two-photon fluorescence microscopy imaging system can achieve rapid detection and regulation of neural circuits. But neural activity rapid detection of the fluorescent dye selection and labeling methods are not mature, the fluorescent signal analysis method is not perfect. This article by screening calcium fluorescent probe and explore its mark method, combined with the random scanning two-photon fluorescence microscopy imaging techniques, to achieve synchronous detection of neurons community activities, calcium fluorescence signal resolve to rebuild the electrical activity of neurons community . Optical detection of the electrical signals of the neurons of the brain slices, demonstrates the random scanning two-photon fluorescence microscopy with high spatial and temporal resolution; using simulated data, static fluorescent samples and brain slices of neuronal calcium fluorescence signal, random scanning system and line scan mode the SNR of the data comparison, and confirmed the flexibility and signal-to-noise ratio of the random scan mode adjustable characteristics. The joint detection of the brain slice neurons membrane potential and calcium fluorescence signal by the amplitude of the fluorescence signal of the comparison high-frequency electrical stimulation induced neuronal calcium and fall times, as well as static fluorescence intensity screened Fluo-5F and Fura-2 as a rapid detection of the the calcium fluorescent dyes; electrode followed by injection of calcium dye method to achieve a mark neurons communities calcium fluorescent dye; relatively high-frequency electrical stimulation of neurons in different parts of the calcium response signal, reveal cortical pyramidal neurons yuan away from the cell body 50 ~~ 00μm apical dendrites the best parts of the high-frequency activity detection, to solve the neuronal calcium signal detection recording site selection problem. Calcium fluorescence signal analysis based on template matching and differential method, parsing and reconstruction of the electrical activity of the calcium fluorescent signal for cell populations. Fura-2 fluorescence signal data, and confirmed that the template matching method can effectively resolve the Fura-2 fluorescence signal data through the simulation data the analog detection as well as the experimental data detection, template matching method to detect; propose and establish assay based on differential Fluo-5F fluorescence signal , to achieve effective reconstruction of the labeled neural circuits in the electrical signal of the Fluo-5F. In addition, the study of single cells in neural circuits, often need to activate or inhibit specific neurons. Direct irradiation of femtosecond laser capable of inducing the activity of nerve cells, neural circuits non-contact, non-destructive, convenient and effective control, the establishment of a neural circuit connected optical discrimination method to map out the functional connection topology diagram of neural circuits.

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CLC: > Medicine, health > Basic Medical > Medical science in general > Biomedical Engineering > Other branches of biomedical engineering > Light,laser biomedical
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