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Population Genetic Structure and Method of Constructing Core Collection for Castanea Mollissim Blume

Author: MaYuMin
Tutor: ChenXueSen;ShuHuaiRui
School: Shandong Agricultural University
Course: Pomology
Keywords: Chinese wild chestnut Phenotypic data Fluorescent AFLP markers Population genetic structure Genetic diversity Core collection
CLC: S664.2
Type: PhD thesis
Year: 2009
Downloads: 369
Quote: 8
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Chestnut (the Castanea mollissim Blume) originated in mainland China, germplasm resources are very rich. In the long-term ecological adaptation process, the Chinese chestnut is formed around the different geographical and climatic conditions of different ecological groups, divided into the Yangtze River, north, northwest, southeast, southwest of ecological groups. Wild chestnut is considered to be the origin of the native species of the world's cultivated chestnut, played a decisive role in the process of domestication of the world's cultivated chestnut, and there are a lot of excellent rare type. However, for the past 20 years, due to the huge demand of the domestic and foreign markets, the chestnut thoroughbred industrialization process accelerated forward from the wild and semi-wild germplasm seed intensive cultivation of wild resources have been seriously damaged, resulting in a number of valuable germplasm disappear . The principles and methods of this paper, the use of molecular systematics, combined with traditional phenotypic research system, the use of fluorescent AFLP markers on the the wild chestnut population genetic structure and genetic variation analysis, and application of molecular markers and phenotypic data, respectively, of the wild chestnut core collection, the findings on scientific conservation and efficient use of this precious resource, and rich biological theory of evolution is important. The main findings are as follows: 1. Conducted a survey of the Chinese chestnut ecological zones of the Qinling Mountains, TOPUNION mountain ecosystems and ecological zones of the Yanshan Mountains of three kinds of populations of 118 wild strains of leaves, fruit morphology and nuts nutrients Research results show that the coefficient of variation of the three populations of leaf size, petiole length and diameter, as well as fruit shape, size and other morphological traits were more than 10%, showing the rich genetic diversity, leaf area and the single grain weight variation range and coefficient of variation; fruit shape index variation range and the smallest coefficient of variation is more stable botanical characters, consistent trend of variation of the three populations; 3 populations moisture 118 wild strains flesh, content of total sugar, starch, protein, fat, ash and vitamin C and other nutrients in the plant difference significant, the coefficient of variation of 6.2% - 28.3%, of which the largest range of variation and coefficient of variation of the protein content of nuts, genetic rich diversity, consistent trend of variation of the three populations; 2. Genetic Variation of the same traits of wild chestnut in three different ecological zones of the Qinling Mountains TOPUNION Mountains and Yanshan Mountains that: the same trait not cohabitation populations most significant or very significant differences in leaf area and Weight minimum Qinling Mountains populations were 56.34cm2 and 2.95 g vitamin C content (96.7mg/100g fresh fruit) are Yanshan mountains and the TOPUNION Mountains populations of 2.29 times and 2.22 times. Correlation analysis showed that single seed weight and leaf area with rainfall and longitude significant positive correlation, a significant negative correlation with the altitude, the vitamin C content and rainfall significantly negatively correlated positively correlated with altitude, indicating that the environment chestnut table Characters of genetic variation has a greater impact; the Yangtze River ecological groups, ecological groups in North China, Northwest ecological groups, southeast of ecological groups and southwest ecological group of 120 Chinese chestnut strains as test materials, the use of fluorescent AFLP markers population genetic structure of wild chestnut five ecological groups studied, the results show that: the EcoR I / Mse I primer (which MSE I primers for the FAM fluorescent markers) average amplified polymorphic loci 180.88, average polymorphic percentage point to 83.31%. Five ecological groups of polymorphic points and polymorphic point percentage: the Yangtze River ecological groups (A = 150.38; P = 73.80%) gt; the North ecological group (A = 147.75; P = 71.93%) gt; northwest ecological group (A = 137.88; P = 68.00%) gt; southeast ecological group (A = 90.50; P = 50.32%) gt; Southwest ecological group (A = 86.00; P = 48.29%); Chinese chestnut in the species level level Nei's gene diversity (H = 0.206) and Shannon's information index (I = 0.326) significantly or very significantly higher than the population level. On a population level, the Yangtze River ecological groups Nei's gene diversity and Shannon's information index (H = 0.191; = 0.302) higher than the North ecological groups (H = 0.185; = 0.295) and the Northwest ecological groups (H = 0.182; I = 0.289), but no significant difference, significantly higher than the southeast (H 0.142; the I = 0.229) and southwest of the ecological group (H 0.139; the I = 0.218); the Chinese wild chestnut five ecological groups of groups of genetic diversity and population genetic structure analysis, the Yangtze River ecological Genetic diversity is the most abundant, reveals the possibility of the Yangtze River Chinese chestnut centers of genetic diversity; 4 In this study, the the wild chestnut population genetic structure parameters --- --- genetic differentiation and gene flow were analyzed. 5 ecological groups, wild chestnut coefficient of genetic differentiation (Gst = 0.0952), wild chestnut genetic variation exists within populations, accounting for 90.48% of the total variation, and the genetic variation among populations accounted for only 9.52% of the total variance. . Calculated by the coefficient of genetic differentiation Gst gene flow Nm = 4.752, wild chestnut five ecological groups there is a moderate gene flow model of the formation of the spatial structure of this genetic variant may be long-distance gene flow, natural climate, geographical distance and isolation the combined result of various factors; wild chestnut five ecological groups groups Nei's genetic identity between 0.8876 - 0.9665, the genetic distance between 0.0341 - 0.1193, indicating a higher degree of similarity between the groups, genetic distance. small. Five ecological groups UPGMA method of cluster analysis, southwest and southeast of ecological groups together first, followed by North and Northwest ecological community together, and finally the Yangtze River ecological groups together with the northwest and northern biological assemblage, this the dendrogram intuitively that the southwest of ecological groups and southeast of ecological groups the highest similarity, genetic relationship; 6. genetic diversity data for 19 of the 297 strains of wild chestnut phenotypic traits to explore the use of table type traits for constructing the the wild chestnut core collection method, the results show that the Mahalanobis distance clustering better than the Euclidean distance, five kinds of clustering methods compared with the group average method, differential square method and the method and the longest distance on the Method the shortest distance method and intermediate distance method, the degree of deviation better than random sampling strategy and preferred sampling strategy. Build core collection using principal component analysis (PCA) and its further confirmation, the distribution of core germplasm throughout the principal component diagram, and away from the center, with specific traits strains are selected as the core lines to ensure the representative of the core collection. This study shows that when the sampling ratio of 20%, using the Mahalanobis distance, away from the differential square method and the method stepwise clustering, combined with the degree of deviation sampling strategy to build the core collection of the most representative is using morphological data The best way to build a core collection of wild chestnut; 7. fluorescent AFLP markers, 120 Chestnut wild strains were used to study the use of molecular markers to build Chinese core collection of wild chestnut. With the control of the random sampling strategy, allele preferred sampling strategy can build a more representative core collection. The principal coordinates on sites preferred sampling strategy to build the core collection representative to confirm the distribution of core collection is displayed throughout the entire plot to ensure the representativeness of the core collection. When you select the 25 lines, according to a number of clustering, SM, Jaccard or Nei Li genetic distance Allele preferred method is to build a more appropriate method of wild chestnut core collection.

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