Dissertation > Excellent graduate degree dissertation topics show

Understanding the Structural Shifts of Gut Microbiota in Patients with Chronic Viral Hepatitis B

Author: LiuJiaZuo
Tutor: ZhaoLiPing
School: Shanghai Jiaotong University
Course: Microbiology
Keywords: gut microbiota chronic viral hepatitis B V3 region of 16S rRNA genes barcoded 454 pyrosequencing Multivariate statistical analysis
CLC: R512.62
Type: Master's thesis
Year: 2011
Downloads: 223
Quote: 0
Read: Download Dissertation

Abstract


Chronic infection of Hepatitis B virus (CHB) still is a global infectious disease with serious sequelae. Recent evidence suggests that among many factors that contribute to the pathogenesis of hepatitis, gut microbiota plays an important role in exacerbation of inflammation and fibrosis in the liver. The aim of this study was to assess the differences between the composition of the gut microbiota in humans with CHB and Health individuals as control.Fresh faecal samples from 300 CHB patients and 149 healthy control individuals were collected. 851,302 sequences of microbial V3 region of the 16S rRNA genes were examined by using bar-coded 454 pyrosequencing technology and clusted into 3754 operational taxonomic units (OTUs) with a similarity cutoff at 97%. The pyrosequencing data were analyzed with multivariate statistical methods and the result showed that significant structural changes occurred in the gut microbiota with CHB. Overall, the gut microbiota of CHB patients was associated with a decrease in the level of diversity compared with the healthy individuals. The abundance of Bacteroidetes was significantly increased, whereas Firmicutes was reduced in the CHB group compared to the Health (P < 0.01). Furthermore, in the genus level, the abundance of Prevotella was remarkably enriched and gut barrier-protecting bacteria Bifidobacterium as well as Roseburia were reduced in CHB (P < 0.01).In conclusion, the results of this study indicate that significant structural shifts of gut microbiota were occurred in the CHB patients. These shifts were caused by reduced bacterial diversity and alteration of some key populations including Prevotella, Roseburia, Bifidobacterium, etc.

Related Dissertations

  1. Effect of Probiotics on Growth Performance, Digestive Enzymes Activities, Immunity and Gut Microbiota of Epiniphelus Coioides,S917.4
  2. Molecular Ecological Studies of the Gut Microbiota Communities Structure of the Grasshopper,S433.2
  3. Quality Traits of Tomato Germplasm Resources Genetic Diversity,S641.2
  4. Relationship of Magnetic Resonance Diffusion-weighted Imaging to Histology in Chronic Viral Hepatitis,R445.2
  5. Co-variation Analysis of Human Gut Microbial Structure and Host Global Metabolism,Q938
  6. Positive liver clear yellow piece of clinical research in the treatment of acute and chronic jaundice viral hepatitis ( damp license ),R259
  7. Studies on EDXRF Analysis of Ancient Ceramics and Data Processing Methods,K854
  8. Of Shugan spleen and blood particles clinical study for the treatment of chronic hepatitis B ( stagnation and spleen deficiency blood stasis ),R259
  9. Diagnosis Value of Serum Hyaluronic Acid for Liver Fibrosis in Patients with Chronic Viral Hepatitis: A Systematic Review,R575.2
  10. Establishment and Characterization of a Chronic Viral Hepatitis Model Induced by Murine Hepatitis Virus Type 3,R-332
  11. Study on the Relationship between Forest Stand Structure and Fuel Characteristics Region of Beijing,S762
  12. Integrated Evaluation Model of the New Socialistic Country and Its Application,F224
  13. Application and Research of Grey Clustering on Estimation of City Competitiveness,F224
  14. The Application Research of Fuzzy Discriminatory Analysis in the Forecast of PIH,R311
  15. Research on Human Resource Development and Economic Development in Gansu Province,C964.2
  16. Statistical Analysis on the Sound Characteristics of Machine Tools,TG502
  17. The Research on Taste Compounds of Green Tea,TS272
  18. Huize Pb-Zn structure geochemical characteristics and metallogenic prediction,P618.4
  19. The Study of Speaker Recognition Based on Principal Component Analysis and Linear Discriminant Analysis,TP391.4
  20. The Study of Batch Process Monitoring and Fault Diagnosis Based on the Theory of Multivariate Statistic and Its Applications,TQ062.1
  21. The New Method of the State-transition Detection Based on Sequences of System Calls,TP393.08

CLC: > Medicine, health > Internal Medicine > Infectious disease > Viral infections > Viral Hepatitis > Hepatitis B
© 2012 www.DissertationTopic.Net  Mobile