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Expressions of 5-HT—5-HT2A R—PKC Pathway in Hypoglossal Nucleus in Normal Rats

Author: ChenYang
Tutor: LiZhuYi；LiuYingYing
School: Fourth Military Medical University
Course: Neurology
Keywords: 5-HT 5-HT2AR SERT PKC P-PKC-Sub Hypoglossal nucleus
CLC: R56
Type: Master's thesis
Year: 2011
Downloads: 15
Quote: 0
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Abstract


The purpose of: the respiratory motion persistent compensatory enhance the long-time the facilitation (long-term facilitation can LTF) called breathing, the breathing of the body to adapt to the needs of the physiological or pathological neural plasticity changes. Phrenic nerve and hypoglossal nerve intermittent hypoxia-inducible expression of LTF is dependent 5-HT 2A R (5-hydroxytryptamine 2A receptor). However, in the past, the main application of electrophysiological methods record LTF expression, lack 5-HT/5-HT 2A R morphological study of the system, especially in the hypoglossal nucleus. The hypoglossal nucleus domination on the respiratory muscles, persistent snoring people during sleep can be induced expression of hypoglossal nerve LTF. The study observed 5-HT/5-HT 2A R system and its downstream protein kinase C (protein kinase C, PKC) signal in the expression of the rat hypoglossal nucleus for further study sublingual neural the LTF expression of the functional significance of morphological evidence. Methods: Using immunofluorescence and electron microscopy techniques, observation of the 5-HT, 5-HT 2A R SERT (serotonin, transporter) and PKC, PKC-Sub (PKC substrate) in adult rat hypoglossal nerve Expression of the nucleus. Results: :5-HT-ir (immunoreactive) nerve fibers in the light microscope, the hypoglossal nucleus was single or linear expression ,5-HT 2A R-ir product is mainly distributed in the cytoplasm of the cell film, some distribution prominences, SERT, PKC and PKC-Sub densely distributed in the cell body protrusions can also be seen in a positive mark. Electric microscope :5-HT-ir nerve fibers and cell bodies and dendrites of the hypoglossal nucleus of motor neurons in close contact during visible asymmetric synapses. Immuno-gold particle-labeled 5-HT 2A R, SERT, PKC, PKC-Sub distributed in the cell bodies and dendrites. Cell bodies, immuno-gold particles are mainly located in the endoplasmic reticulum and ribosomes, the membrane can be seen under the gold particles mark. Dendrites, immunogold particles more than along the membrane surface distribution. Particularly noteworthy is the PKC-, PKC-Sub-IR gold particles in the specific distribution of the postsynaptic membrane. The surface of the inner nuclear membrane is also seen with the expression of PKC-Sub. Conclusion: This study provides the rat hypoglossal nucleus motor nerve yuan expression of 5-HT-5-HT 2A R-PKC pathway morphological basis, the mechanism may be involved in the regulation of the upper respiratory tract muscles tension and hypoglossal nerve LTF expression, especially in hypoxia, hypercapnia and asphyxia intervention case. PKC, PKC-Sub postsynaptic densely distributed, suggesting possible via phosphorylation postsynaptic protein involved in the regulation of respiratory plasticity.

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CLC: > Medicine, health > Internal Medicine > Respiratory system and chest diseases
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