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Aflatoxin B1 (Aflatoxin B1, AFB1) is dominated by aflatoxin (Aspergillus flavus), Aspergillus parasiticus (Aspergillus parasi ticus) and a set of peaks Aspergillus (Aspergillus nomius) produce secondary metabolites , in peanuts, wheat , cotton , corn and rice and other crops is widespread . AFB1 is the strongest kind of chemical carcinogens mutagens , toxic and highly carcinogenic , can cause the animal stomach, liver, kidney , etc., can also be transferred between organisms through the food chain to humans and livestock health threats. Many countries have provisions in their various food , food and compound word limit standard , therefore , research is accurate, sensitive , rapid AFB, detection method has important practical significance. This study established the indirect competitive chemiluminescent enzyme immunoassay (Chemiluminescence enzyme immunoassay, CLEIA) detection of food aflatoxin B1, the main contents are as follows : an analysis of the antigen coating concentration , closed condition , the antibody concentration and reaction time for the competitive impact of the reaction system after optimization of the indicators for the AFB1-BSA antigen coating concentration 1.5μg/mL, 4 ℃ coated 12h-15h; 5% skim milk , 37 ℃ closed 1h; dilution of a monoclonal anti- AFB1 : 150000 ; HRP secondary antibody dilution of 1:2000 ; competitive reaction time 45min. 2 CLEIA indirect competitive method to establish the linear range of the 15.63 pg/mL-500.00 pg / mL between the linear regression equation was y = 23.264x-43.206, R2 = 0.9924; detection limit of 8.67 pg/mLIC50 was 54.96 pg / mL, intra-and inter- average coefficients of variation were 4.03% and 6.97% ; average recovery was 92.72% , the average coefficient of variation was 8.68% . 3 were used indirect competition AFB1-CLEIA AFB1-ELISA method and a commercially available test kits quantitative determination of 32 samples ( 5 samples of wheat , rice samples 8 , corn samples 8 , peanuts 11 ) , of which 28 samples were AFB1 contamination , AFB1 content is 132 ng/kg-3626 ng / kg, the two methods showed no significant difference in P lt; 0.01, R2 = 0.9954, two methods shows the test results without significant difference . This method than the commercially available AFB1-ELISA kit 5.76 -fold increase in sensitivity .
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