Dissertation > Excellent graduate degree dissertation topics show

The Protection of the Adult Rat Cardiac Myocytes in Vitro and Serum-free Culture with TH and the Study of It’s Relationship with the Expression of Fas/FasL

Author: ZhuShuQiang
Tutor: XuJianJun
School: Nanchang University
Course: Surgery
Keywords: T3 Rats Myocardial cells Primary cultured Fas / FasL
CLC: R329
Type: Master's thesis
Year: 2011
Downloads: 4
Quote: 0
Read: Download Dissertation

Abstract


Objective: To establish in vitro serum starvation cultured adult rat cardiac myocyte model , using drugs triiodo thyronine (T3) intervention , observation T3 serum-free culture adult rat myocardial cells have a protective effect and the relationship between the expression of Fas system . Methods: serum-free M199 medium primary cultured adult rat myocardial cells , cell morphology was observed under the light microscope , trypan blue staining evaluation cell activity , counting myocardial cells were randomly divided into a control group and T3 group were cultured for 48 hours . Streaming cytometric detection of myocardial apoptosis , Western blotting Determination of Fas, FasL protein expression changes in myocardial cells . Results: Each adult SD rat cell yield available (4-7) x 106 , of which 75 % -85 % after recalcification activity ventricular myocytes , cells were rod-shaped , clear stripes , refraction sharp edges at both ends , which the spontaneous contraction of 5% -10 % of the rod-shaped cells in serum-free cultured adult rat cardiac myocytes ; T3 has a protective effect : myocardial cells in serum-free culture for 48 hours , the flow cytometry myocardial cells wither death the T3 group myocardial apoptosis rate (13.26 ± 2.46%) compared with the control group (44.32 ± 6.12%) was significantly lower ( P lt; 0.05 ) ; Western blotting analysis results suggest that the T3 group compared with the control group, Fas , FasL expression was decreased . Conclusion : T3 can reduce the serum-free cultured adult rat cardiac myocyte apoptosis may be weakened the cardiac myocyte Fas / FasL gene expression .

Related Dissertations

  1. Effects of CrPyr on Protein Metabolism in Rats,S865.12
  2. The Protective Effect of Rat Myocardial Ischemia/reperfusion Injury Following Bone Marrow Mesenchymal Stem Cell Pretransplantation for 1 Week,R542.22
  3. The Effects of Atorvastatin on Expression of Osteopontin in Kidney of the Diabetic Rats Its Molecular Mechanism,R587.2
  4. Effects of Cumulus Cells on Maturarion in Vitro and Subsequent Developmental Competence of Porcine Oocytes,S828
  5. Meridians of Asthma Thread Burying Model Rats IL-4 Such Impact Experimental Study,R245
  6. The Study of the Effects of Isoflavones of Puerarin and Daidzein on Bone Metabolism in Ovariectomized Female Rats,R580
  7. Effect of Microwave on GAP-43 Expression in the Spinal Cord after Sciatic Nerve Lesions in Rats,R651.3
  8. The Expression and Mechanism of NF-κ B and IL-6 after 90% Portal Vein Ligation in Rats,R657.3
  9. In Vitro Culture and Identification of Vascular Smooth Muscle Cells from Rat Intra- and Extracranial Arteries,R743.3
  10. Effects of Different Species Meats on Growth, Digestion Metabolism and Antioxidant Status in Rats,S865.12
  11. Effect of Grape Seed Procyanidins on the Blood Pressure in Renovascular Hypertensive Rats and Its Mechanism,R544.1
  12. The Research on Treatment of Lentivirus-mediated hVEGF-165 Gene Transferring Mesenchymal Stem Cells on Rat Hind Limb Ischemia,R329
  13. Relationship between the Severity of Heart Failure and Alteration of Serum Thyroid Hormone Concentration and Peripheral Blood Leukocyte Count in Acute Myocardial Infarction Patients,R542.22
  14. Experimental Study of Neural Stem Cells around the Lateral Ventricular Zone of the Hydrocephalus Rats,R742.7
  15. Anti-gout Capsules on the Treatment of Gouty Arthritis and Its Mechanism,R259
  16. Experimental Study of GUI QIN MISTURE on Adriamycin-induced Nephropathy in Rats,R285.5
  17. Effects of Losartan on Na~+, K~+-ATPase in Proximal Tubules Epithelial Cells of Spontaneously Hypertensive Rats,R544.1
  18. Morphological Characteristics of Astrocytes in EAN–a Tricellular System for Study on Neuroprotection and Neuroregeneration,R651.1
  19. Rat hepatoma evolution of three biochemical factors and related drugs Intervention,R735.7
  20. Effect of Different Glucose Concentrations on Autophagy in Neonatal Rat Cardiomyocyte Culture,R542.2
  21. The Effects of High Protein Feeding on the Brain Development of IUGR Rats,R714.5

CLC: > Medicine, health > Basic Medical > Human morphology > Human histology
© 2012 www.DissertationTopic.Net  Mobile