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The paeoniflorin hair silver plants peony root extract, extensive pharmacological effects, mainly anti-inflammatory, immune, kidney, liver protection, anti-depressant and influence cell proliferation role, accounting for 90% of total glucosides of peony. Clinical study found that the exact effect of TGP on the treatment of psoriasis. Psoriasis is a chronic inflammatory, proliferative skin diseases, good hair in young, stubborn disease, recurrent and progressive increase constitutes a great harm to the human body. Pathological performance the keratinocytes (keratinocyte KC) excessive proliferation and differentiation of abnormalities, inflammatory cell infiltration and dermal capillary proliferation, extend, moved to song and expansion. STAT-3 involved in intracellular signal transduction, play an important role in the regulation of cytokine network, regulation of gene expression of numerous cytokines and inflammatory mediators, regulation of immune responses, to participate in the pathogenesis of psoriasis. Therefore, STAT-3 is an important target in the treatment of psoriasis. Main purpose of this thesis is to study cell proliferation caused by Paeoniflorin after in vitro activation of STAT-3 and STAT-3 activation, and downstream cytokine expression of MMP-2, VEGF secretion increased the impact, in order to clarify new angle from the STAT-3 The mechanism of action of the Paeoniflorin, provide a basis for Paeoniflorin used in the treatment of psoriasis and epidermal proliferative skin disease in clinical and experimental basis. For the above purpose, design of experiments, the results are as follows: (1) TNF-a-induced HaCaT cell proliferation, activation of STAT-3 and MMP-2, VEGF secretion: HaCaT cells induced with TNF-a 10ng/ml, inverted microscope HaCaT cell morphology changes in the cells of the stimulation group compared with normal control group than the number of cells increased significantly, no significant difference in cell morphology; the MTT colorimetric detection rate of cell proliferation TNF-a stimulation of cell proliferation, significantly up to 201%. Western-blot detection activation of STAT-3 (p-Stat-3), found by unstimulated no p-Stat-3 expression of TNF-a stimulation of p-STAT-3 expression was significantly; quantitative PCR STAT- the 3mRNA STAT-3 downstream cytokines active MMP-2 and VEGF mRNA expression and found that after stimulation of STAT-3 mRNA expression did not change significantly, MMP-2, VEGF expression was significantly enhanced. The ② Paeoniflorin proliferation of HaCaT cells induced by TNF-a, STAT-3 activation and expression of MMP-2, VEGF secretion: the design of the experimental group intervention group concentration gradient group and time gradient group. Inverted microscope morphological changes observed concentrations and time HaCaT cells were normal cell morphology of each concentration and the time group, no significant difference between the various concentrations than stimulation with TNF-a, the cell density is slightly sparse intervention most evident after 24h ; the MTT colorimetric detection Paeoniflorin intervention proliferation of HaCaT cells after stimulation by TNF-a inhibition and found Paeoniflorin induced HaCaT cell proliferation inhibition rate in a time-and concentration-dependent manner; western-blot method to detect the concentration changes in the activation of STAT-3 (p-Stat-3), and found that various concentrations of activated STAT-3, no significant reversal of the role; fluorescent quantitative PCR detecting the intervention group on the STAT-3mRNA Preparation cells and activation of STAT-3 factor MMP-2, VEGF mRNA expression and found that the intervention group had no significant effect on STAT-3 mRNA expression, HaCaT cells after stimulation of MMP-2 and VEGF mRNA expression of a certain degree of down. In summary, the experimental results show that paeoniflorin is not only possible to suppress the TNF-a-induced proliferation of HaCaT cells, and can be lowered psoriasis cytokines VEGF, MMP-2 expression, but the activation of STAT-3 was no significant reversal effect.
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