|
Objective: To screen the Eucommia anti-skin photoaging effective parts; pharmacodynamics and mechanism of skin photoaging active fraction. Methods: macroporous resin column Eucommia extract is separated into two parts: water elution (WE) and alcohol-eluting site (EE), UVA and UVB irradiated HaCaT and ESF-1 cells photoaging model, cell morphology, cell activity vitality LDH.SOD, MDA content as an indicator, screening the Eucommia anti-skin photoaging effective parts; using the parts of both oral and topical role in the light aging model mice, observed tissue morphology, SOD activity and MDA content; role of the site in the UVA irradiation of ESF-1 cells photoaging model, the detection of the enzyme-linked immunosorbent assay (ELISA method) cell culture medium and MMP-1.MMP-3.TIMP-1 type I collagen (Col I), elastin (ELA) content: WE HaCaT and ESF-1 cells photoaging model were not significantly affected. EE can significantly improve the UVA, UVB light aging HaCaT and ESF-cell activity, reducing the LDH activity in the culture medium and the content of MDA, increased SOD activity. EE after oral administration to improve mouse skin tissue aging, improve SOD activity in mouse plasma, reduce the content of MDA, but still visible in mouse epidermis and thickening of the stratum corneum, dermal layer of fibrous tissue or broken or cracked, curled kink phenomenon. Topical, it is possible to improve the organization of mouse skin aging, improve SOD activity in mouse plasma, decreased MDA content. Tissue damage was significantly reduced, closer to normal tissue, the dermis more complete fibrous tissue, rare fracture, broken, does not appear to curl kinks. EE 250μg · mL-1 can inhibit the abnormal secretion of MMP-1 and elastin UVA irradiation ESF-1 cells, stimulate collagen synthesis, but had no effect on the secretion of TIMP-1 and MMP-3. Conclusion: EE on UV-induced photoaging HaCaT.ESF-1 cells have a protective effect, to determine EE anti-skin photoaging parts. EE has the pharmacological effects of anti-skin photoaging, and topical than oral administration. Speculated that the mechanism can scavenge oxygen free radicals, improve oxidase activity, to protect the cell membrane structure, suppress the abnormal secretion of matrix metalloproteinases and elastin, promote collagen synthesis. This study effectively deepen the the Eucommia anti-skin photoaging of basic research, is the Eucommia used in the prevention and delay skin photoaging provide a theoretical basis.
|