Dissertation > Excellent graduate degree dissertation topics show

Modification of the Methods in 15N Tracer and Their Use in Determination of Rumen Microbial Protein Synthesis

Author: GaoZhanFeng
Tutor: LuoXuGang；ZhaoGuangYong
School: Chinese Academy of Agricultural Sciences
Course: Animal Nutrition and Feed Science
Keywords: 15N Calculation method Rumen microbial protein Non-protein nitrogen
CLC: S852.21
Type: PhD thesis
Year: 2010
Downloads: 107
Quote: 0
Read: Download Dissertation

Abstract


This paper by three research to improve 15 N isotope analysis techniques and methods of computation, on the basis of this study, used in ruminant protein metabolism studies, by artificial rumen short-term fermentation, use 15 N labeling technique of urea synthesis efficiency of rumen microbial protein and urea in the rumen digesta of each component in the distribution, to lay the foundation for 15 N-labeled widely used. Research ZHT-03 spectrometer scan control and data acquisition and processing system on the basis of the original circuit ZHT-03 mass spectrometry appropriate improvements, by the computer, software, and data acquisition card for data acquisition and scan control system its digital transformation and achieve automatic control, analog-to-digital conversion with 16-bit A / D. The 15 N abundance of 0.366% -5.264% 4 standard sample series at different times of six determinations. The results show that the: After transformation determination of accuracy and precision than before the transformation have been significantly improved the accuracy of the standard sample series were between 99.80% -99.95%, especially low-abundance sample (, 15 n% lt; 1%) the accuracy of the determination has been significantly improved; analysis efficiency is improved to more than 1 times; analysis process dynamic display, and automatic data processing and storage, easy to sort out the analysis results. Application in the field of agricultural research laid the foundation for 15 N isotope. Research the two 15 N isotope tracer Improvement of calculation method according to 15 N abundance of the atomic weight of nitrogen for 15 N isotope tracer studies in experimental design, results, error calculation and analysis, combined with chemical and isotopic theory, a theoretical analysis in terms of the amount of markers, total nitrogen, and methods of computation Ndff nitrogen dimensionless. Proposed in the original calculation method based on the nitrogen metabolism of nitrogen mol-dimensional marker usage, total nitrogen results and Ndff the calculation method eliminates the original calculation method itself is the cause of error, thereby improve the accuracy of the 15 N isotope tracer technique, to provide theoretical guidance for the scientific use 15 N isotope tracer techniques to study. Research three-utilization 15 N mark Determination of urea in the rumen into the MCP efficiency through artificial rumen-term and short-term fermentation, 4 × 2 two-factor completely randomized design, four urea levels: 15 N-labeled urea instead of dietary crude protein, 0%, 10%, 20%, 40%; 2 fermentation time points: 24h, 48h. Urea instead of a different proportion of dietary protein synthesis of rumen microbial protein efficiency. The results showed that: 1 urea instead of the proportion of dietary protein had no significant effect on rumen fluid pH, concentration of ammonia nitrogen and undigested feed dry matter; ruminal pH and ammonia nitrogen concentration of the significantly increased with the extension of the fermentation time, not digestion of feed dry matter significantly reduced; 2 urea in the rumen digesta number of the distribution of each component and its percentage in the total nitrogen ratio of the component with the urea instead of a higher proportion of dietary protein significantly increased; the 3 urea synthesis microbial efficiency to urea instead of 10% of the dietary CP, fermentation 24h, the highest efficiency of the urea synthesis of microbial protein, 53.6%; the 4 dietary urea in the distribution of the number of each component in the rumen chyme order supernatant gt; undigested feed gt ; the liquid microbial gt; particle phase micro-organisms.

Related Dissertations

  1. Sludge Incineration Technology Research of Petrochemical Enterprise,X703
  2. Study on Protein Quality Evaluation of Raw Milk and Milk Products,TS252.7
  3. Establishment of the Protein Risk Index System of Milk and Dairy Procucts,TS252.7
  4. Study of Fertilization with Fertilizer Applicator on Nitrogen Absorption, Distribution and Growing Characteristics of Peach Tree,S662.1
  5. Absorption and Distribution of Nitrogen Treatment and Its Effect on Yield and Quality in Zhonghuashoutao Peach,S662.1
  6. Plain river network area hydrology and flood impact assessment method for calculating Discussion,TV122
  7. Test Research on Variable Amplitude Fatigue Behavior of Grid Structure with Welded Hollow Spherical Joints,TU356
  8. The Research on Speed Sensorless Drives of Induction Motor,TM346
  9. Effect of Different Degradation Speed of Energy and Protein Feed Sources on Rumen Fermentation Characteristic,S816
  10. Studies on Characteristics of Absorption, Distribution and Utilization of Different 15N-labeled Fertilizers in Rubber Tree Seedlings,S794.1
  11. Comparative Studies of Super Hybrid Rice Ratoon Materials Transport Disciplines,S511
  12. Numerical Simulation Analysis of STFC Half-through Arch Bridge,U448.22
  13. The Bearing Capacity and Structural Analysis of Rock-socketed Piles Under the Geological Condition in Chongqing,TU473.1
  14. Plant Growth Promoting Rhizobacteria Cultivation Optimization and Mechanism Research,Q93
  15. The Study of Ecological Water Demand in River Basin,X143
  16. Research for Reasonable Audience Number of Museum,TU242.5
  17. Mineral development ecological compensation fee calculation methods and case studies,F426.1
  18. The Research of Theoretical Analysis and Experiment on Expansion Reinforcing Strip,TU755
  19. The bench blasting parameters Biden Phosphate reasonable selection and Blasting Fragmentation Distribution Law,TD235.3
  20. Ankang City 10KV Reactive Power Optimization of,TM714
  21. Load-Carrying Capacity and Practical Calculation Method of Welded Hollow Spherical Joints Subject to Combined Axial Force and Bending Moment,TU391

CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Animal Medicine ( Veterinary Medicine) > Basic Veterinary Science > Animal physiology, biophysics,biochemistry > Veterinary Physiology,
© 2012 www.DissertationTopic.Net  Mobile