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The Research on Stress Response Mechanism of Hypothalamus-Pituitary-Adrenal Axis in Different Exercise Stress State

Author: LiuZhiYuan
Tutor: ZhangLin
School: Suzhou University
Course: Physical Education and Training
Keywords: Exercise Stress HPA axis Mechanism GR NOS
CLC: R87
Type: PhD thesis
Year: 2010
Downloads: 324
Quote: 1
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Abstract

Research Objective: Exercise Stress is a biological basis of sports training and sports and fitness effects, but the exercise stress hypothalamic - pituitary - adrenal axis (HPA) axis less central response mechanism, especially for different sports stress Nitric oxide (NO) by the glucocorticoid receptor (GR) glucocorticoid hormones (GC) combined to affect the HPA axis regulation, and GR NOS changes in the central regulation of the HPA axis system has not been reported. In this study, due to different load treadmill exercise stress study, at different levels and angles of the weekly changes in hormone levels, from the HPA axis the central receptor gene level changes and protein expression change, analysis of different load exercise stress HPA The shaft of the degree of stress and central regulation law to reveal the mechanism of the central regulation of different exercise load stress. Methods: 130 11-week-old male SD rats were randomly divided into a long-term exercise group, once the exercise group and the control group. Long-term exercise group rats with high, medium and low three different load treadmill training 6 days / week, 1 hour / day experimental period of 8 weeks;, a campaign group has also adopted three different load exercise, the use of long-term training group equivalent load last exercise program. Test methods and indicators: double antibody sandwich enzyme immunoassay serum of CORT, T, ACTH, hypothalamic CRH content; immunohistochemical method to measure hippocampal CA1 region of the hypothalamic arcuate nucleus, ventromedial nucleus, dorsomedial nucleus paraventricular nucleus, central amygdala morphology, and determination of the GR, NOS positive neurons change; RT-PCR assay hippocampus, hypothalamus, amygdala GR, the MR, NOS gene transcription level determined by Western blotting of the nuclei GR NOS protein expression. Results: (1) long-term large load sport hippocampal MR, GR transcription decreased GR expression decreased (P lt; 0.05) no significant change in the the NOS transcription and expression levels; the hypothalamus excited improve, GR, MR transcriptional level decreases, NOS expression increased (P lt; 0.05); amygdala GR no significant change in the level of gene transcription, the amygdala NOS gene transcription increased (P lt; 0.05); ACTH elevated CRH, CORT concentration decreased. (2) Long-term Moderate Exercise hippocampal GR NOS gene transcription and expression increased (P lt; 0.01), hippocampal excitatory reduced; the hypothalamic excitability increase, GR transcriptional reduce the, protein concentration, increased NOS gene transcription and expression (P lt; 0.05); amygdala increased excitability, GR, NOS gene transcription and decreased expression of various nuclei reduce MR gene transcription; CRH, ACTH, CORT concentration (P lt; 0.05). Hippocampus (3) long-term low-load exercise group had no significant change in the MR gene transcription increased GR transcriptional level decreased NOS gene transcription and expression increased (P lt; 0.05); hypothalamus excitement increased NOS, GR gene transcription and expression (P lt; 0.05); amygdala NOS gene transcription and decreased expression of the GR gene transcription and expression of no significant changes in the amygdala and hypothalamus to reduce MR gene transcription, CRH, CORT concentration, ACTH was no significant change. (4) increased at a large load sport hippocampal NOS gene transcription and expression of the GR gene transcription increased MR gene transcription decreased hippocampus reduced; decrease the excitability of hypothalamic NOS gene transcription and expression increased, reduced GR and MR gene transcription , GR protein levels (P lt; 0.05); decrease the excitability of the amygdala, NOS, reduced GR gene transcription and expression, and an increase in the MR gene transcription (P lt; 0.05); CRH, CORT concentration of ACTH concentration decreased (P lt; 0.05). (5) a Moderate Exercise hippocampal NOS gene transcription and expression increased GR gene transcription and expression levels decreased in hippocampus no significant change; hypothalamic paraventricular nucleus excited NOS gene transcription and increased expression of the GR gene transcription level without changes in the expression CRH, CORT concentration increased (P lt; 0.05); increased amygdala GR gene transcription and expression of NOS gene transcription decreased, but the NOS concentration, the nuclei MR transcription lower (P lt; 0.05); , ACTH concentration decreased. (6) a low-load exercise on hippocampal excitability did not change, NOS, reduced GR gene transcription and expression of MR gene transcription did not change; the hypothalamic excitability increase NOS no significant changes in gene transcription, increased expression of NOS increased GR gene transcription and expression ; increased excitability of the amygdala, an increase in NOS gene transcription and expression, no significant changes in the GR gene transcription, expression increased, the amygdala and hypothalamus MR gene transcription decreased (P lt; 0.05); CRH, CORT concentration of ACTH concentration decreased (P lt; 0.05). Conclusions: (1) long-term load exercise stress of hippocampus, hypothalamus and amygdala excitability decreased, HPA axis suppression; thalamus and amygdala increased excitability under a heavy load caused by exercise stress, HPA axis activation. (2) long-term load movement in hippocampal suppression is caused due to the hippocampus injury; a large load sport in hippocampal inhibition is due to the the peripheral high level of GC negative feedback regulation. No correlation (3) Sports stress response of hippocampal excitability with NOS, NOS only participate in the hippocampus injury, do not participate in the excitability of adjustment. (4) long-term Moderate Exercise with a medium load exercise stress HPA axis main difference is that ACTH change the long-term moderate load ACTH increased amygdala NOS is involved in the regulation of pituitary ACTH decreased after a moderate load is mainly affected by peripheral GC under hypothalamus and amygdala GR negative feedback regulation due. (5) A small load movement in hippocampal excitability, without significant change, the increased excitability of the amygdala and hypothalamus, amygdala NOS participation in the long-term small load movement pituitary ACTH regulation, load ACTH decreased by GC with feedback regulation of hippocampal GR . (6) long-term movement of the HPA axis, the central regulation of the stress response than peripheral regulation; HPA axis feedback regulation of various aspects of a campaign mainly dominated.

The Research on Stress Response Mechanism of Hypothalamus-Pituitary-Adrenal Axis in Different Exercise Stress State

Abstract

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