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Studies on Inhibition Effects of Extracts from Bryopsis Plumose and Enteromorpha Linza on the Tyrosinase

Author: ShangFei
Tutor: YanCuiWei
School: Ocean University of China
Course: Biological Engineering
Keywords: Yu algae Edge of the tube Enteromorpha Tyrosinase Inhibition Dynamics
CLC: R285
Type: Master's thesis
Year: 2011
Downloads: 59
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Abstract

The tyrosinase (EC.1.14.18.1) is a widely distributed among microorganisms, plants and animals as well as human enzyme associated with the formation of melanin. Tyrosinase having the monophenol activity and activity of diphenols in the organism, the the monophenol activity performance the monophenol plus oxygen formed diphenols, polyphenols enzyme activity performance will be oxidized to form a quinone of diphenols polyhydric phenol, these quinone substances poly-protein interactions and intracellular Finally, the formation of melanin. Tyrosinase acquired immune response for organisms melanin formation and intrusion of foreign objects produce a vital role. Screening has a high efficiency can inhibit the activity of tyrosinase inhibitors used in the treatment of skin pigment, R \u0026 D whitening cosmetics, bio-pesticides and food additives from natural seaweed. In this study, 11 species of marine algae tyrosinase inhibitory activity screening, Yu algae and the edge of the tube Enteromorpha extract on tyrosinase inhibition. Determined by orthogonal experiments, the optimum extraction conditions both seaweed extract, organic solvent extraction separation of the different components of the two kinds of seaweed extract through kinetic learning method analysis of the inhibition of tyrosinase type The mechanism research content and results are as follows: 11 species of marine algae tyrosinase inhibitory activity screening. 5mL, the total volume of the screening system and the substrate dopa content of 1.0mmol / L phosphate buffer pH 6.8, the final concentration of the tyrosinase solution 1.28ug/mL inhibitor a final concentration of 4mg/mL reaction temperature of 37 ° C, test the reaction time of 1min. The results show that in 4mg/mL extract concentrations, Rhodophyta the fork sticks algae true Jiang Lei, centipede algae, coralline algae, duck feather algae and fan intends Iraq algae tyrosinase inhibition The role of inhibitory activity were higher than 20%. Tine algae and true Jiang Lei tyrosinase inhibition rate more than 40% of Yu algae Chlorophyta, Ulva pertusa edge of the tube, Enteromorpha showed different degrees of inhibition of tyrosinase, which Yu algae highest inhibition rate of 78%, followed by the edge of the tube Enteromorpha 40.24%, the Rhodophyta twigs domoic and the Phaeophyta carrageenan showed tyrosinase reverse activation, activation rate 18.18% and 20.45%, respectively. 2 constant temperature water extraction method to extract the chemical composition of the feather alga Enteromorpha and the edge of the tube, the extraction solvent is ethanol solution extraction liquid ratio 1:12, extraction temperature of 40 ° C. First single factor experiment to determine the best value of the single extraction conditions, then the use of orthogonal experiment to determine the best combination of extraction conditions. Feather algae best combination of extraction conditions: 60 ° C constant temperature water bath, 60% ethanol concentration, extraction time of 5h and 1:14 material-liquid ratio; edge tube Enteromorpha best combination of extraction conditions: 60 ℃ water bath, 60% ethanol concentration, extraction time of 5h and 1:10 solid to liquid ratio. Two seaweed ethanol extracts sequentially extracted by the organic solvent to obtain the ethyl acetate extract, petroleum ether extracts and water-soluble components, respectively, determination of the inhibitory activity of the three components, the type of inhibition and polyphenol content. The results show that: half of Yu algae petroleum ether component of the tyrosinase inhibitory concentration IC ₅₀ 2.1mg/mL, noncompetitive reversible inhibition, inhibition constant the K _I = 0.33; Yu algae ethyl acetate component of tyrosinase half inhibitory concentration IC ₅₀ for 0.27mg/mL is a non-competitive reversible inhibition, inhibition constant for K _I = 0.13; Yu algae water-soluble component of the the Tyrosinase half inhibitory concentration IC ₅₀ 1.0mg/mL a competitive reversible inhibition, inhibition constant: K _I = 0.91. The edge tube Enteromorpha petroleum ether extract half of tyrosinase inhibitory concentration IC ₅₀ 0.29mg/mL, a competitive reversible inhibition, inhibition constant: K _{I = 0.28; edge tube Enteromorpha ethyl acetate component of tyrosinase half inhibitory concentration IC ₅₀ for 0.19mg/mL, is a non-competitive reversible inhibition, inhibition constant for K _I = 0.08; edge tube Enteromorpha half of the water-soluble component of tyrosinase inhibitory concentration IC ₅₀ 7.02mg/mL, a competitive reversible inhibition, inhibition constant K _I = 2.95. Feather algae ethyl acetate component of tyrosinase inhibition strongest edge tube Enteromorpha ethyl acetate component highest inhibitory activity of tyrosinase.}

Studies on Inhibition Effects of Extracts from Bryopsis Plumose and Enteromorpha Linza on the Tyrosinase

Abstract

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