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Extraction, Separation and Idification of Antifouling Active Substances in Ulva Pertusa

Author: LiuHuiHui
Tutor: FuYuBin；ZhengJiYong
School: Ocean University of China
Course: Materials Physics and Chemistry
Keywords: Ulva pertusa Biofouling Natural product antifouling Natural product extraction and separation Structural identification
CLC: R284
Type: Master's thesis
Year: 2011
Downloads: 32
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Abstract


Biofouling is the thorny issue of the maritime sector has been plagued by losses worldwide each year caused by fouling organisms can not be estimated. Defaced creature increases its own weight and the frictional resistance of the hull, so that the sailing speed reduced, seriously affect the maneuverability of the ship. Also the fuel consumption increases, the costs rise, and increase the maintenance costs of the ship, to shorten the life of the ship, the sea biological metabolism of medium will cause the corrosion of the hull, to affect the safety of the ship. So to prevent fouling of the sea creatures become an important issue must be addressed. The ship the shell underwater part of the most effective anti-pollution measures is painting antifouling paints, antifouling purpose of traditional antifouling paints has reached toxic organic tin, cuprous oxide and other toxic antifouling agent, so the marine environment, causing serious pollution. With the people's awareness of environmental protection, the new environmentally friendly antifouling coatings is becoming a hotspot. Antifouling research of marine natural products attracted renewed attention because of its the unique antifouling mechanism of action and efficient antifouling activity. Ulva pertusa as our common along the coast of the Yellow Sea and Bohai large algae, which itself has the ability to prevent other fouling organisms attached. The thesis of Ulva pertusa as the object of study, with the help of modern chromatographic separation and analysis technology, extracted from Ulva pertusa separation antifouling active substances, and then by means of infrared spectroscopy, nuclear magnetic resonance spectroscopy, gas chromatography - mass spectrometry on Ulva pertusa extract structure to analyze and identify, and provide an important basis for the subsequent development of new antifouling agent. Ethyl acetate extract first hole Ulva UPAFS-A after secondary silica gel column chromatography to give the antifouling strongest UPAFS-A52, UPAFS-A52 were treated with ethanol, chloroform / acetonitrile, hydrochloric acid / ethyl acetate After, get the four product UPAFS-A521, UPAFS-A522 the UPAFS the A523, UPAFS-A524. In this paper, the plume Navicula, Mytilus edulis, Ulva spores and white ridge barnacle larvae typical fouling organisms detected antifouling activity of Ulva pertusa extract and segment separation and purification products. The results showed: (1) UPAFS-A Navicula attached plume completely inhibited the minimum concentration of 0.7 mg / mL, completely inhibited the blue mussel byssus attached to a concentration of 0.4 mg / mL completely inhibited the adhesion of Ulva spores the concentration of 0.4 mg / mL. (2) sixth product UPAFS-A separated by column chromatography obtained pinnate NAVICULA with inhibition of the best part is UPAFS-A3 segment inhibition rate reached more than 90% in the 0.3 mg / mL, followed UPAFS -A5, 0.6 mg / mL, the inhibition rate reached more than 90%; Mytilus edulis byssus threads experiment, UPAFS-A3 was completely inhibited at 0.3 mg / mL blue mussel byssus attachment, UPAFS-A5 0.5 mg test results of inhibition of Ulva spores / mL completely inhibited the adhesion of the blue mussel byssus, UPAFS-A6 0.4 mg / mL completely inhibit the attachment of the blue mussel byssus the Mytilus edulis cytotoxicity; show UPAFS-A3 of spores separatist growth inhibition rate reached 90% concentration of 0.7 mg / mL, UPAFS-A5 0.2 mg / mL, UPAFS-A6 0.3 mg / mL; the white ridge barnacle larvae experimental results show that: UPAFS-A3 pharmacodynamic strongest, dialogue ridge barnacle larvae lethal concentration ≤ 0.1 mg / mL. UPAFS-A5 0.4 mg / mL the white ridge barnacle larvae death LC50 was 0.287 mg / mL. UPAFS-A6 0.4 mg / mL death all white ridge barnacle larvae can LC50 was 0.311 mg / mL. (3) UPAFS-A3, UPAFS-A5 the eight product was obtained by silica gel column secondary chromatography antifouling ratings: the antifouling activity strongest part the is UPAFS-A52, and 0.3 mg / mL completely inhibited pinnate funagata algae, blue mussel byssus attachment also can completely suppress the Ulva spores separatist growth; the white ridge barnacle larvae experimental, UPAFS-A52 toxicity weakest still the white ridge barnacle larvae survival when 0.8 mg / mL, the LC50 was 0.550 mg / mL, and UPAFS-A51 in 0.4 mg / mL the white ridge barnacle larvae have all died, the LC50 of 0.217 mg / mL; UPAFS-A31 in 0.2 mg / mL white ridge barnacle larvae died The number greatly increase the mortality rate as high as 86.7%, the LC50 of 0.158 mg / mL. (4) after UPAFS-A52 purification the product UPAFS-A521 the UPAFS-A522, UPAFS-A523 the UPAFS-A524 the plume suppression NAVICULA, experimental show the plume Navicula these four product to promote adhesion, the the antifouling ability to significantly reduce . UPAFS-A521 ~ A524 by gas chromatography - mass spectrometry, infrared spectroscopy, nuclear magnetic resonance spectroscopy detection UPAFS-A521 and UPAFS-A522 contains saturated palmitic acid, UPAFS-A523 salt crystals contain saturated, UPAFS-A524 in palmitic acid and saturated and 16 ethyl. Combination of the plume NAVICULA antifouling evaluation experiments found saturated palmitic acid, saturated sixteen ethyl does not have anti-fouling ability, or they need to play with other substances UPAFS-A52 synergies to better antifouling effect.

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