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Water Extract and Acohol Extract of Turmeric’s Protective Effection and Its Mechanism Research to the Rat with the Acetic Acid Injurily Ulcerous Colitis

Author: ChenWenHua
Tutor: FangChengKang；HuangGuoDong
School: Nanchang University
Course: Traditional Chinese Medicine
Keywords: Turmeric water , alcohol extract Ulcerative Colitis Malondialdehyde Superoxide dismutase Spasmolytic polypeptide
CLC: R285.5
Type: Master's thesis
Year: 2011
Downloads: 23
Quote: 0
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Abstract


Objective animal experiments observed turmeric water, alcohol extract of glacial acetic acid injury in ulcerative colitis (UC), recognize identified with anti-UC-associated cytokines from the reaction of oxygen free radicals (OFR) perspective to reveal turmeric water, alcohol extract of UC mechanism. 180 method of clean grade rats were randomly divided into nine groups (n = 20): normal group, model group, sulfasalazine (SASP) group the turmeric water extract (WET), in the low-dose group and turmeric alcohol extract (AET), the low-dose group. SASP group was given SASP solution 180mg/kg · d-1 body weight orally, once a day; the turmeric water extract and alcohol extract high, medium and low-dose group were given turmeric water extract 80mg/kg · d-1, 40mg / kg · d-1, 20mg/kg · d-1 and the alcohol extract liquid 80mg/kg · d-1 40mg/kg · d-1, 20mg/kg · d-1 body weight orally once a day. All animals were treated for 2 weeks. The first three weeks were decapitated, calculate the gross morphology colonic mucosa injury scores. Blood collection 4ml, plasma malondialdehyde (MDA) content and superoxide dismutase (SOD) activity. Taken near the anus 8cm at the colon, conventional HE staining biopsy and camera to calculate the colonic mucosa histological injury scores, immunohistochemistry (IHC) detection colonic mucosa spasmolytic polypeptide (SP) expression. Results colonic mucosa of UC model rats gross morphological and histological injury score and plasma MDA content than those of normal rats increased plasma SOD activity decreased colonic mucosa SP expression raised. Compared with model group, turmeric alcohol extraction liquid high, middle dose group (alcohol is high in the group) junction mucosa general morphological and histological damage score and plasma MDA content were significant with reduce, plasma SOD significantly with increased, junction mucosa SP of expression significantly lowered (P lt; 0.01), with the SASP group comparison without significantly with statistical difference (P gt; 0.05); turmeric alcohol extract liquid low-dose group (alcohol low group) and water extract liquid high-dose group (water high group) colon mucosal general morphological and histological damage score and plasma MDA content significantly reduce plasma SOD significantly with elevated expression of the colonic mucosa SP significantly with lowered (P lt; 0.01), SASP group comparison there is a significant statistical difference (P lt; 0.01); aqueous extract low-dose group (water, low group) in the colonic mucosa gross morphological and histological injury score, plasma SOD activity and expression of the colonic mucosa SP model group showed no statistically significant differences (P gt; 0.05 ). Conclusion ① The experiments confirmed the intestine perfusion acetic acid-induced ulcerative colitis model is reliable. ② high, middle dose (80mg/kg · d-1 (?) 40mg/kg · d-1) turmeric alcohol extract can promote ulcer healing of acetic acid injury ulcerative colitis in rats. The mechanism by to increase superoxide dismutase vitality, reduce malondialdehyde content, improve the body's ability to scavenge oxygen free radicals, increase colonic mucosa stability, thereby enhancing the antioxidant free radical damage in the ability of the colonic mucosa, reduce lipid peroxidation reaction injury, to prevent the generation of the ulcer. The spasmolytic polypeptide expression in colonic mucosa of patients with ulcerative colitis, mucosal repair is closely related to and parallel with the degree of damage of the colonic mucosa. ③ turmeric water extract can not promote ulcer healing of acetic acid injury ulcerative colitis in rats, but still have the role of a larger dose (80mg/kg · d-1).

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