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Diabetes (Diabetes Mellitus, DM) has become a serious global public health problem, and a growing threat to people's health. Looking for new drugs with high efficacy, side effects, is still a focus of the current field of diabetes research. Chinese medicine treatment of diabetes in China has a long history, improvement of clinical symptoms, to control chronic complications of diabetes as well as auxiliary hypoglycemic effect clear. And has a rich source of toxicity, easily accepted and long-term use of the advantages. Supina Potentilla supina Linn. Rosaceae Rosaceae Potentilla species. The whole plant medicinal, distributed all over the country. Attending functions hematemesis, hematuria, bloody diarrhea, blood in the stool, beard and hair as early as white teeth is not solid. In our country folk as the the edible wild utilization, Medicinal and Edible Plants. In recent years, the plant used in the treatment of diabetes in the civil, and the effect is remarkable. But no reported overturned Committee Potentilla hypoglycemic effect and chemical composition. The experiment more systematic study of the hypoglycemic effect and its chemical composition supina find the compounds were found to have hypoglycemic effect of new drugs to provide experimental data and theoretical resources for further expansion of the diabetes medication, developed diabetes basis. The experiments on the overturned Potentilla dishes were used to heat reflux extraction and cold soak extraction ethanol extract, and then the ethanol extract with petroleum ether, ethyl acetate, n-butanol for extraction. By alloxan-induced diabetic mouse model experiments to determine the hypoglycemic effect and hypoglycemic supina effective parts of the ethyl acetate extract. By silica gel column chromatography, Sephadex LH-20 gel column chromatography, etc. Modern chromatographic method further separation system of two kinds of ethyl acetate extract, separated therefrom to obtain a monomer compound (8). The structures were identified based on the physicochemical properties and the use of 1H-NMR, 13C-NMR modern pop learning method. 8 monomer compounds were: to Rose acid (euscaphic acid, 1), Potentilla acid (tormentic-acid, 2) myrianthic acid (3), daucosterol (daucosterol, 4), esculetin (esculetin, 5 ), apigenin (apigenin, 6), multiflora rose glycosides (rosamultin, 7), astragalin (astragalin, 8). According to the literature compound 1,2,3,4,6,8 has a hypoglycemic effect. Compounds 1-8 are isolated for the first time from supina.
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