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The Mechanical Research about the Influnence of Boron to the Hypothalamic-Pituitary-Ovary Axis of Ostrich Chicks

Author: WangYan
Tutor: PengKeMei
School: Huazhong Agricultural University
Course: Animal Genetic Breeding and Reproduction
Keywords: Boric acid Young ostrich Hypothalamic Pituitary Ovarian Apoptosis
CLC: S839
Type: PhD thesis
Year: 2008
Downloads: 133
Quote: 1
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Abstract


The African ostrich main life in Africa and the Arabian desert hot. Female ostriches in 2-2.5y only reach sexual maturity, mature no later than the animal poultry neutral, its the Brood very long, 3mon. What is the reason so that the ostrich sexual maturity so late? Gonads must have its unique traits. Taking estrogen allows serum 17β-estradiol, serum ceruloplasmin and triglyceride levels significantly higher complement boron, increased more obvious; description of boron with estrogen-like effects and can enhance estrogen function. Thus, whether by adding boron to improve the effectiveness of ostrich farming has important scientific significance. In view of this, the test animals to the African young ostrich adding boric acid 0mg / L, 100 mg / L, 200 mg / L, 400 mg / L, using transmission electron microscopy, radioimmunoassay, histochemistry, Immunohistochemical localization of expression a variety of methods, from the various levels of the cells, subcellular, serum hormone levels, protein analysis and in situ nick end labeling, immunoblotting research the female underage ostrich under hypothalamic - pituitary - ovarian axis (HPO) organizational structure characteristics as well as the addition of boric acid, HPO axis mechanism. The main findings are as follows: 1 boron affect the hypothalamus of the female young ostrich addition of boric acid as a test animal, test 45d female young ostrich hypothalamus in paraffin sections, HE staining, immunoblotting, immunohistochemistry SABC method of MT1 young ostrich under distribution of positioning, the study boron the young ostrich hypothalamus and the role. The results show that the number of nucleated cells per unit area in the hypothalamic paraventricular 400mg / L group was the highest, the difference was not significant. Large, medium-sized cells ranged from 200mg / L was the highest, significantly higher than the other three groups; supraoptic nucleus number of cells per unit area in the 200mg / L group was the highest, with the control group compared to the very significant difference. Large and medium-sized cell rate 200mg / L group was the highest, 400 mg / L group mainly mostly small cells; hypothalamic arcuate nucleus cells per unit area of ??the highest number in the control group, 200mg / L group, followed by a significant difference; 400mg / L group at least, the difference is extremely significant, compared with the control group, a significant difference. The control group is the majority of small cells. The paraventricular nucleus melatonin receptor immunoreactive cells (MT1ir) cells in positive intensity expression, 200mg / L the most positive expression difference significant weakest expression of 400mg / L group MT1ir strength, a significant difference; supraoptic The number of nuclear MT1ir up to 400mg / L group, intensive, but the average integrated luminosity minimum, incomplete cell morphology, a significant difference; the arcuate nucleus the control group MT1ir cells the largest number of densely arranged. 100mg / L group average integrated luminosity highest, most positive expression, the difference was not significant. 400mg / L group, the average integral photometric minimum, the difference is significant. The results show that the paraventricular nucleus, supraoptic nucleus has a significant role in the 200mg / L group, but 400mg / L significantly inhibited; arcuate nucleus has a smaller role in the 100mg / L group, 400 mg / L, the group has significant inhibition. 2 boron female young ostrich pituitary the Peacse pituitary stained, immunoblotting, immunohistochemistry SABC method to locate adding boron young ostrich adenohypophysis cells and MT1 hypothalamus distribution of. Peacse law results show that the more the number of eosinophils in the control group, clear cell outline, triangular or circular. The basophilic cells vary in size, cytoplasmic basophilic granules contain. The chromophobe number, small cell volume pale, ill-defined cells. The the boron additive 100mg / L group the basophilic cells increase in the number of. Up to 200mg / L group, the number of basophilic cells. 400mg / L group eosinophils, basophils into the group distribution of the minimum number of chromophobe; the SABC method results the control group adenohypophysis MT1ir cells were shades ranging from yellow-brown, irregularly shaped cell body, piles or scattered positive immune reaction particles are mainly distributed in the nucleus, cytoplasmic coloring. The cell number 100mg / L group adenohypophysis MT1ir increased, the strong immunoreactive cells. The cells increased number 200mg / L group adenohypophysis MT1ir immunoreactive cells were strongly positive. The positive cells 400mg / L group adenohypophysis MT1ir reduced, immunoreactive cells mainly weakly positive. The results show that: add boron 100mg / L, 200 mg / L, group can increase the number of basophils; Add 100 mg / L, 200 mg / L of boron after MT1ir cells showed an increasing trend in number, strength enhanced, but 400mg / L group MT1ir positive cells inhibition. 3 boron young ostrich ovary impact produced by light microscopy and electric mirror sample, extraction of total protein, HE, immunohistochemistry SABC immune blotting method to study female young ostrich ovary of the histological structure characteristics and adding boric young ostrich ovary of . The results show that, add Add 100mg / L boron able to increase ovarian primary oocyte cell diameter, the difference is very significant, 100mg / L boron able to significantly with the number of increasing the primordial follicles, 200mg / L able to significantly with the increase of primordial follicles, primary follicles, secondary The number of follicles and the follicle follicle cells and oocytes MT1ir of cell strength than the control group significantly enhanced, 400mg / L can significantly reduce the number of primordial follicles. 4 after adding boron in serum E 2 P the 4 changes using RIA technologies, adding boron 15d, 30d, 45d after detection underage ostrich serum E 2 , P 4 changes. The results showed that adding boron 15d, 100 mg / L, 200 mg / L group and the control group compared to serum E 2 the P 4 levels are elevated, the difference was not significant . The lower E2 levels of 400 mg / L group, a significant difference the P4 the difference was not significant; adding boron 30d, 100 mg / L, 200 mg / L, and 400 mg / L group and the control group compared to the serum E 2 , P 4 level differences are not significant. Add boron 45d after 100 mg / L, 200 mg / L group serum E 2 levels were compared increased to 200 mg / L group difference was very significant level of 400 mg / L group The lower, the difference was not significant. 200 mg / L, 400 mg / L group P of 4 levels of the control group compared to the lower, the difference is significant. The results show that the appropriate amount of boron to increase the level of serum E 2 , but in the added amount of 400 mg / L will reduce serum E 2 level; adding boron 15 P 4 -30d smaller, but added to the 45d when serum P 4 levels were reduced. 5 boron the female underage ostrich hypothalamic - pituitary - ovarian axis apoptosis in situ nick end labeling (TUNEL) detection technology of adding different doses of boron the young ostrich HPO axis system the study normal young ostrich apoptosis law and boron this axis apoptosis and the young ostrich vivo apoptosis tissue positioning. The results show that boron can produce a significant role in the supraoptic nucleus, the arcuate nucleus, pituitary and ovarian 100,200 mg / L can significantly reduce the apoptotic index, 400 mg / L boron can significantly increase the apoptotic index . Boron on the hypothalamic paraventricular nucleus in each group, the difference was not significant.

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