Dissertation > Excellent graduate degree dissertation topics show

Ginsenoside Glycosidases from Panax Ginseng

Author: ZhangChunZhi
Tutor: AnLiJia;JinFengZuo
School: Dalian University of Technology
Course: Biochemical Engineering
Keywords: Ginsenoside -β- glucosidase Ginsenoside -α- Arab refined glycosidase Ginsenoside glycosyl hydrolase Enzyme purification Enzymatic hydrolysis Ginsenoside Rh2 Ginsenoside Rd
CLC: TQ461
Type: PhD thesis
Year: 2002
Downloads: 621
Quote: 0
Read: Download Dissertation


Specific hydrolysis ginsenoside-specific glycosylation, separation and purification of ginsenosides glycosyl hydrolase, nature study, and ginsenoside Rg3 and Rc directed enzymatic reaction proved Enzymatic directed transformation of ginsenoside structure possibilities. Buffer extraction, ammonium sulfate precipitation, ion exchange chromatography two ginsenoside glycosyl hydrolase - Ginsenoside-β-glucosidase and ginsenoside-alpha-arabinosidases enzyme isolated and purified from ginseng roots. Ginsenoside-β-glucosidase was purified 16-fold, a yield of 14.4%, ginsenoside-α-arabinoside enzyme was purified 18.5-fold, a yield of 4.6%. PAGE analysis of the purified enzyme a single band. Of ginsenoside-α-glucosidase hydrolysis the ginsenoside Rg3 generation ginsenoside Rh 2 , the optimum temperature was 55-60 ℃, the optimum pH was 5.0 Ca activation of the enzyme, Cu inhibition. Enzyme is stable below 60 ℃, pH4.0-7.0, SDS-PAGE and measured molecular weight of 59kDa. N-terminal amino acid sequence of this enzyme is SLDANYVPKYVTLPL, no homology with known sequences of the β-glucosidase, ginsenoside-β-glucosidase in the hydrolysis characteristics different from the traditional β-glucosidase (EC3. 2.1.21). The article also ginsenoside-β-glucosidase catalytic the Rg3 reaction of the Michaelis-Menten equation. Ginsenoside-α-arabinosidases hydrolytic and ginsenoside Rc generated ginsenoside Rd optimum temperature of 50 ° C, the optimum pH was 5.0, Cu enzyme inhibition. Enzyme is stable below 60 ℃, pH4.0-6.0, SDS-PAGE and measured molecular weight of 86kDa. The ginsenoside Rg3 ginsenoside glycosyl hydrolase hydrolysis substrate concentration of 10mg/ml, pH5.0, 55 ℃ conditions, reaction The 24h of ginsenoside Rg3 conversion rate can reach 60%. Saturated n-butanol extraction, silica gel column, hydrolysates Rh 2 purity of up to 90%, and the yield was 32%. Ginsenoside-β-glucosidase hydrolysis of 20 (S)-Rg3 and 20 (R)-Rg3 respectively generate 20 (S)-Rh 2 and 20 (R)-Rh 2 , roughly the same rate of hydrolysis. Magnetic resonance imaging ( 1 HNMR, 13 CNMR) and mass spectrometry (MS) detection results show that the resulting product of the enzymatic hydrolysis 20 (S)-Rg3 20 (S)-Rh < sub> 2 , system name is 3-O-(β-D-glucopyranosyl-yl) - dammar -24 - ene-3β, 12β, 20 (S) - triol; enzymolysis 20 (R )-Rg3 resultant product 20 (R)-RH 2 , system name is 3-O-(β-D-pyran-glucopyranosyl) - dammar -24 - ene-3β, 12β, 20 (R) - triol, molecular formula is C 36 H 62 O 8 , a molecular weight of 622. Ginsenoside glycosyl hydrolase substrate concentration 30mg/ml, pH5.0, 50 ℃ conditions hydrolysis ginsenoside Rc, the reaction 24h, RC the conversion rate reached 60%. Saturated n-butanol extraction, silica gel column, the hydrolysates Rd purity of 90%, a yield of 34%. Nuclear Magnetic Resonance ( 1 HNMR, 13 CNMR) and mass spectrometry (MS) detection results show that hydrolysates of 20 (S) - ginsenoside Rd, and the name of the system for 3 - O-(β-D-glucopyranosyl - (1 → 2)-β-the D-glucopyranose yl)-20-O-(β-D-pyran-glucopyranosyl) - dammar -24 - Summary alkenyl and p, 126,20 o Bu triol, molecular weight formula C48H82Ol to 946. In the prove enzymatic directional conversion of ginseng soap with the preparation of rare soap bitter feasibility, laid the experimental foundation for the further study of sugar hydrolase soap bitter glycosyl hydrolases.

Related Dissertations

  1. Haihong Fruit Vinegar Processing Technology,TS264.22
  2. The Research on the Comprehensive Modification Technology of Rice Protein Enzymatichydrolysis and Graft Copolymerization,TS201.2
  3. Cellulose Pretreatment with Ionic Liquid for Enzymatic Hydrolysis,TQ352.1
  4. Study on Extraction Process and Properties of Collagen Witch Extract from the Skin and Swim Bladder of Bighead Carp,TS254.9
  5. Technological Research on Preparation of High Activity Walnut Pulp by Ultrasonic-compound Enzyme Synergistic Method,TS255.6
  6. Preparation and Physicochemical Properties of ACE-inhibitory Peptides from Silkworm Pupae Protein,S886.9
  7. Study on Application and Development of Osmanthus Fragrans Resources with Enzymatic Hydrolysis Method,TQ654
  8. Dirhamnolipid Enhanced Enzymatic Hydrolysis of Lignocellulose in Compost,TQ352.1
  9. Effect of Magnetic Fe3O4 Particles and Study of the Cellulase Immobilized by Magnetic Gelatin on Enzymatic Hydrolysis of Sunflower Seed Hull,TQ223.122
  10. The Study of the Separation of Rice Straw Composition and the Fermentation of Its Hydrolyzate to Ethanol,S216.2
  11. The Molecular Mechanism of MCF-7 Cell Apoptosis Induced by Ginsenoside Rh2 and Its New Derivative,R737.33
  12. Bioconveision of Wheat Straw to Fuel Ethanol and Separation of Xylan in Liquid,TQ223.122
  13. Enzymatic Preparation of Flavor-enhancing Peptide from Soybean Meal Protein,TS202.3
  14. Study on the Functional Properties of the Modified Rice Protein by Hydrolysis and Maillard Reaction,TS201.2
  15. Pesticide-degradation Characteristics and Degradation Mechanism of Biocontrol Bacteria,S476
  16. Study on Enzymatic Hydrolysis of Corn Straw and Butanol Fermentation,TQ920.1
  17. The Analysis of Optical Purity of β-tert-Hydroxy Esters/Nitriles/Acids by HPLC and the Preliminary Studies in Enzymatic Hydrolysis of β-tert-Hydroxy Esters,O621.25
  18. Study on Process of Enzymatic Hydrolysis Chinese Pine Pollen Protein for Preparing Small Peptide,TQ464.7
  19. Enzymatic improve soy protein isolate foaming and emulsifying properties of,TS201.21
  20. Isolation and Structure Identification of the extraction of the effective components of Astragalus,R284
  21. Enzymatic salted duck egg white preparation of albumin peptide,TS253.2

CLC: > Industrial Technology > Chemical Industry > Pharmaceutical chemical industry > The Chinese herbal medicine preparations of the production
© 2012 www.DissertationTopic.Net  Mobile