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The Expression of Endoplasmic Reticulum Molecular Chaperons in Mouse Brain during Development

Author: LiBo
Tutor: ChenYuHua
School: China Medical University
Course: Cell Biology
Keywords: Endoplasmic reticulum Chaperone GRP78 GRP94 Brain development Mice
CLC: Q51
Type: PhD thesis
Year: 2003
Downloads: 178
Quote: 0
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Abstract


The purpose of the embryonic period is an important stage in the ontogenetic process of multi-cellular organisms, this period of embryonic susceptible to adverse environmental factors. The stress response is an important protective response organism against adverse external environmental factors, a large amount of the heat shock protein (Heat Shock Proteins of HSPs) are produced within the cell in the course of the reaction, they have to resist damage, protect cells. Studies have shown that, even in the non-stressed state, the cells are still the expression of HSPs, about the role of heat shock proteins in embryonic development process have long been concerned about. The Marie-Therese.Loones, Sandra M.Dsouza studied the distribution of heat shock proteins during the development of the nervous system in the mouse embryonic and postnatal, speculated that HSPs may be related to the differentiation of nerve cells in the neurodevelopmental process, migration, and signal transduction in nerve cells and proteins relating to the transport. 78,94 glucose-regulated protein (Glucose-regulated protein78 94 of GRP78, GRP94 is) is a stress protein, heat shock protein of HSP70 and HSP90 high homology. They molecular chaperone is the cavity of the distribution of the endoplasmic reticulum (Endoplasmic Reticulum ER), having a weak ATP activity, and the ATP-binding and can assist the translocation of the nascent protein folding, and the oligomeric protein assembly. The essence of the development of multicellular organisms is the choice of expression, of the gene expression of the maturation of the protein depends on the assistance of molecular chaperones. Studies have shown that GRP78 in the organism during the development of the fertilized egg cell stage, and already a high level of expression in the blastocyst. Recent studies also suggest that GRP78, GRP94 played an important role in the process of differentiation of embryonic rat myocardial cells and cardiac organogenesis. The endoplasmic reticulum chaperone GRP78, GRP94 is in the process of brain development is so far unclear. Study the relationship between the endoplasmic reticulum chaperone GRP78, GRP94 is mouse brain development from a neurodevelopmental perspective, explore the endoplasmic reticulum chaperone of GRP78, GRP94 is the biological significance of this process. Method 1. RT-PCR was used to detect GRP78, GRP94 mRNA expression in the mouse embryonic brain tissue of different developmental 2. Northern-blot method to detect GRP78 mRNA expression in the E16.5 day mouse embryonic brain tissue protein immunoblot Westem blot analysis and development in different periods and EI 6 .5 days mouse embryonic brain tissue in different parts of GRP78, GR Mao 4 protein Expression. 4. Immunohistochemistry (SP method) detection GRF78 GR bang braided distribution of developmental expression and localization in the different stages of brain tissue in mice. The primary cultured E16.5 mice whole brain induced by the UPR with tunicamycin primary cultured tissues was detected by RT-PCR and Western blot method of GRP78 GR India mR-NA and protein expression. Results. Molecules in mouse brain development partner G Journal of order 8, G Journal of four 4 expression showing chronological, and the expression of the two different modes: GR 8 early embryonic expression, the end of embryonic development decreased increased gradually after birth and one week after birth to reach the level of adult mice, while the 'GR ie 4 in the lower level of expression of the early development of the Developing gradually increased and peaked at birth, after birth, has been maintained at a high level. This indicates that the chaperone GR kinds GR that during development has a different role, and at the same developmental stages, the differences in expression levels prompted some mechanism exists in vivo regulation in specific developmental stages expression of molecular chaperones. 2 mouse brain development of GRP78, GR 4 expression patterns in space is not the same. Showing spatial differences in the expression of GR function in the mouse brain during development, showing a concentration gradient: a space from the end of the brain to brain protein and mRNA expression levels decreased gradually and GRF94 expression does not show such differences, no significant changes in the area of ??brain tissue. This shows GRp78 may be a factor of a position information in the brain during development, establishment procedure involved in the morphology of early brain development. Also during development, GR 8, G Journal 4:4 play a role in different regions of the brain tissue are not the same. GR species in the entire mouse development 8, GR 4 mRNA and protein expression level is always a positive phase related to both the expression and regulation at the transcriptional level, GR] 8 4 4 expression, G Journal mode different from the existence of different signaling pathways to regulate both the expression in the mouse brain development. 4 morphological study of the results found in the entire process of the mouse brain development, GR]-order 8 with the GRP94 protein distribution substantially the same, mainly in the cytoplasm of nerve cells and nerve cells appeared significantly earlier time in the glial cells. The distribution of the nerve cells in line with the central nervous system in the point of view of the stem cells, or neurons and glial cells are derived from a common stem cell, to generate neurons in tissue, stem cells begin to differentiate into glial When the neurons generated after mesenchymal cells. Vitro added UPR inducer tunicamycin primary cultured mouse brain tissue GRf mouth 8 GR or 4 mRNA and protein levels were increased, and GRP78 4 trend consistent with GR, this change in vivo findings, suggesting that the UPR pathway that GR feet 8 and GR 4 has the same role in regulating signaling pathway not yet known regulation of endoplasmic reticulum chaperone expression, but still exist in the body, resulting in brain development GRp GR boast expression trend inconsistent. Conclusion 1. GRP mouth temporal and spatial expression patterns in the mouse brain development, and a concentration gradient distribution in space, it may have a position in the developing brain factor. 4 expression patterns in the mouse brain development 2.GR chronological rather than spatial order. 3.GR feet 8, GR 4 different expression patterns in the developing brain suggests the presence of unrecognized the UPR mechanism different endoplasmic reticulum chaperone expression and regulation mechanisms.

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