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Biological Removal of Odorous Gases by Biofilter and Microbiological Mechanism

Author: YinJun
Tutor: ChenYingXu
School: Zhejiang University
Course: Environmental Engineering
Keywords: Biofiltration Stench Ammonia Hydrogen sulfide Trimethylamine Denaturing gradient gel electrophoresis
CLC: X701
Type: PhD thesis
Year: 2004
Downloads: 929
Quote: 7
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Abstract


On the basis of the preliminary investigation of municipal sewage pumping station stench, the study selected the the major pollutants H fetid urban drainage facilities caused 2 S, NH 3 and the top three amine as an object of study, and deodorization biofilter study explored the mechanism of biological deodorization and the factors that affect the biofilter process running. Deal with ammonia biofilter microbial diversity combining techniques from molecular biology methods in the the stench treatment of microbial research and understanding of the the biofilter microbial species and microbial ecology. Separated by a conventional screening method to obtain the the two trimethylamine efficient degrading bacteria. Selection of the peat biofilter treatment containing low concentrations of H 2 S malodorous gases, the results show that the the peat biofilter effective concentrations of less than <70 mg / m 3 The H 2 S gas, gas residence time greater than 25 sec, the removal efficiency of up to 99% or more, and have a strong impact load and the biofilter. It was found that, due to uneven distribution of filler peat particles, low bulk density, easy compression will lead to the tower pressure drop increases, which will lead to the increase of operating costs in the actual engineering applications. Use of activated sludge composting as a filler to deal with low concentrations of H 2 S and NH 3 gas mixture, the results show that the the compost biofilter can deal effectively with low concentrations of H 2 S malodorous gases, the removal efficiency of up to 99% removal of ammonia in the presence of H 2 S had no effect, but the biofilter system ammonia processing efficiency has been low. Through biodegradable Analysis of the product showed that, during the operation into the biofilter ammonia substantially removed through the physical and chemical effects (adsorption, absorption, and the role), the real ammonia removal by microbial nitrification less. Malodorous gas treatment containing NH 3 , the choice made of pig manure compost and sludge particles as a filler to study the effect of the biofilter long-running process ammonia investigated autotrophic ammonia oxidation ecological types of bacteria with running time changes, as well as the ability to deal with ammonia biofilter under non-steady-state conditions. The study showed that the two fillers used in dealing with the effect of the ammonia-containing gas is preferably within 210 days of the reactor operation. Ammonia, showed a higher ability to remove sludge biofilter start-up time takes about a month, when the operation of the two-reactor reached steady state concentrations of less than 110mg / m 3 However, when the concentration of ammonia continues to rise, the two biofilter for ammonia removal efficiency began to decline when the inlet concentration greater than 190 mg / m 3 , the removal efficiency dropped to 50 percent. Long-running and high load conditions, the two biofilter inactivation phenomenon, which may occur with the nature of the packing structure related to changes product accumulation is packing the changing nature of the factors, that since the product of accumulated salt concentration is too high, cause osmotic pressure effects of inhibition of the activity of nitrifying bacteria. Autotrophic ammonia-oxidizing bacteria through the the vaccination biological filter tower DGGE analysis, found itself compost as is the presence of ammonia oxidizing bacteria, ammonia-oxidizing bacteria in compost biofilter running snail genus nitrosated from the referral knife sosPiramult noon rmis based; sludge biofilter running a small number of ammonia-oxidizing bacteria, when domesticated complete reactor reaches a steady state, ammonia-oxidizing bacteria increased nitrosated the Xanthomonas Ni flail mouth monas eutrl, ha, mainly Type Culture can be seen in two biofilter is not exactly the same. Biofilters treatment of low concentrations of ammonia-containing gas under non-steady state conditions The results show that the biofilter can adapt quickly to the inlet concentration and the sudden change of the gas flow, little influence of load fluctuation of the intake air of Biofilters; through a period of time after the idle restart biofilter, the two biofilter for ammonia removal capacity can be restored in a relatively short period of time, the time required from a few hours to a day ranging from general idle time longer, the longer the time required for recovery processing capacity; Biofilters empty load running for some time and then restarted, the time required for the recovery of ammonia removal capacity than completely stop running start the required time is short; high volume load will result in a significant decrease of the removal efficiency of the biofilter, mainly because of the high concentration of ammonia on the toxic effects of microbes in the biofilter microbial activity recovery takes a long time; filler moisture content of biological filter very important to the normal operation of the tower, once the filler dry removal efficiency dropped drastically. trimethylamine-degrading microorganisms is a corpse no mouth printed cc. Me negative secretin hundred the hil public, Pseudom, mouth. , Field, homicrobium, and so on. This study from two different habitats, trimethylamine as a carbon source enrichment, separation and purification of the resulting two trimethylamine are degrading strains Paracoccus (paracocco sP). Isolates either aerobic conditions decreased Solutions trimethylamine, can also under anaerobic conditions with trimethylamine as a sole carbon source for growth. Strain differences in the physiological and biochemical level and 165 rDNA sequence homology, are close to Paraeoeeus amsnovorans, the with Paraeoccus aminovorans 165 rDNA sequence similarity are 98.8%. Biofilter treatment of malodorous gases trimethylamine results show that the the compost biofilter and sludge biofilter can deal effectively with trimethylamine malodorous gases reach 100%, trimethylamine processing efficiency. During the experiment, the compost can not only effectively handle trimethylamine, can also be completely processed its metabolites ammonia; the sludge only handle trimethylamine, does not have the ability to further degradation of ammonia. Filler metabolites closely related to the distribution of filler, the microbial distribution as well as biochemical reaction, compost biofilter typical order of reaction phenomenon, that the first paragraph of the biofilter degradation of trimethylamine, second paragraph mainly ammonia nitration.

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CLC: > Environmental science, safety science > Processing and comprehensive utilization of waste > General issues > Exhaust gas processing and utilization
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