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Solitary enkephalin in rats with neuropathic pain and acupuncture analgesia effects and mechanisms of solitary FQ (orpahanin FQ / nociceptin, OFQ) is a member of the family of opioid peptides found in 1995, is an opioid receptor-like receptor (opioid receptor -like 1 receptor, ORL1) of the endogenous ligand. It is a 17 peptide (FGGFTGARKSARKLANQ) in a molecular weight of 1810, is similar to a structure and dynorphin A. Classic OFQ and Mu, delta, kappa opioid receptor affinity is very low, nor can be activated with the ORL1 high affinity and activate. The OFQ and their receptors are widely distributed in the central nervous system, involved in pain modulation, endocrine, immune, cardiovascular and other physiological activities. The role of OFQ in the brain and spinal cord OFQ in pain modulation. Numerous studies show that the brain OFQ pain caused by sensitive against morphine analgesia and spinal cord OFQ produce analgesia and enhance morphine analgesia. In the rat model of inflammatory pain, my room past studies have shown that intrathecal injection of OFQ can inhibit the formalin-induced hyperalgesia. Neuralgia as a chronic disease common clinical neuropathic pain, can cause central nervous system plasticity. OFQ as a member of the family of opioid peptides, neuralgia what changes occur is not clear. My room sciatic nerve chronic restrictive injury (chronic constriction injury, CCI) induced rat model of neuropathic pain (with 4-0 catgut sciatic nerve Songzha the the four, the formation of limiting damage, three days after thermal hyperalgesia after 7 days to stabilize, and can continue for more than 4 weeks is generally the use of animal models of neuropathic pain in a domestic and international), preliminary research suggests that intrathecal injection of OFQ can inhibit neuropathic pain in rats hyperalgesia phenomenon, but the exact mechanism is unclear. In addition, a lot of practice acupuncture analgesia is an effective analgesic method is widely used in clinical. Acupuncture for chronic neuropathic pain (eg sciatica) have a good effect. Over the years, studies have shown that the mechanism of acupuncture analgesia, acupuncture is by activating the body endogenous pain modulation systems, such as opioid peptides sky analgesic effect. The OFQ What is the role acupuncture treatment of neuropathic pain, what role do? Therefore, to explore the role of OFQ in rats with neuropathic pain and acupuncture analgesia is the purpose of this research project. This topic is proposed on the basis of previous experimental further reveal the analgesic effect of the spinal cord of OFQ neuralgia rats to study spinal cord OFQ electroacupuncture analgesia observed neuropathic pain and electro-acupuncture treatment of neuropathic pain, spinal cord and brain OFQ and its receptor changes. The neuralgia process, central nervous system plasticity change, which increased the activity of the glutamate system is an important factor that caused the rats with neuralgia Min. Experiments show that the cAMP-dependent protein kinase (PKA) is involved in neuropathic pain process, the mechanism OFQ analgesia cells, whether through the cAMP-PKA pathway is still lt; WP = gt; unclear. Therefore, to explore the relationship between the OFQ glutamate system, initially revealed OFQ role in cellular signal transduction pathways is the subject of another study. In short, this project intends to CCI neuralgia rat model, learned from the behavior of the different aspects of the morphology, from the body to different levels from the body, the use of in situ hybridization, immunohistochemistry, fluorescence double-labeling and Western-Blot such as the analgesic effect of molecular biology techniques to observe OFQ and electricity for neuropathic pain in rats, researchers OFQ and its receptor in neuropathic pain and acupuncture analgesia changes, initially revealed the OFQ mechanism of action and intracellular signal transduction pathway. The results are as follows: spinal cord OFQ 1.1 intrathecal injection in rats with neuropathic pain and electroacupuncture analgesia OFQ neuralgia of rats with neuralgia threshold sciatic nerve ligation 7 days, respectively, in the intrathecal injection 3, 10, 30 microg OFQ, 0,10,20,30,60,90 min after administration of pain measurement, observation heels latency (paw withdrawal latency, PWL) changes. The results show: OFQ available dose-related increase the neuralgia pain threshold in rats, a significant analgesic effect. 1.2 intrathecal injection [Nphe1] nociceptin (1-13) NH2 and impact of nocistatin neuralgia OFQ analgesic effect of neuralgia sciatic nerve ligation 7 days after intrathecal injection of 10 mg OFQ, 10 microg OFQ by antagonist [Nphe1] nociceptin (1-13) NH2, 10 microg OFQ 10 microg [Nphe1 of nociceptin (1-13) NH2, 10 mg OFQ function antagonist nocistatin, 10 μg OFQ 10 μg nocistatin, saline, to after the drug the 0,10,20,30,60,90 min measured pain, observe the the PWL change. The results showed that: OFQ receptor antagonist had no significant effect neuralgia pain threshold in rats, but can block the analgesic effect of OFQ on neuropathic pain in rats. Similarly nocistatin had no significant effect on the pain threshold of neuropathic pain rats, but also can block the analgesic effect of OFQ on neuropathic pain in rats. 1.3 intrathecal injection [Nphe1] nociceptin (1-13) NH2 and postsurgical nocistatin electroacupuncture analgesia neuralgia seven days to give EA 30 min observation PWL change. The results showed: EA can significantly reduce neuropathic pain hyperalgesia in rats. EA 5 min before intrathecal injection 10 microg [Nphe1] nociceptin (1-13 of) NH2 and 10 microg, nocistatin after administration 0,10,20,30,60,90 min pain measurement, observe the PWL changes. The results showed: [Nphe1] nociceptin (1-13) NH2 can partially block the EA lt; WP = gt; analgesic effect on neuropathic pain in rats, nocistatin can partially block the power on neuropathic pain in rats town role of pain. 1.4 intrathecal injection of OFQ electroacupuncture analgesia neuralgia postsurgical days, intrathecal injection of OFQ while giving EA 30 min after administration 0,10,20,30,60,90 min measured pain, observe the PWL changes. The results showed that: OFQ can enhance the analgesic effect of electroacupuncture on neuropathic pain in rats. Summary: intrathecal injection of OFQ neuralgia rats analgesic effect and dose-related. OFQ receptor antagonists and functional antagonists can block the OFQ analgesic effect, suggesting that intrathecal injection of OFQ analgesic effect through its receptor. EA analgesic effect can be OFQ receptor antagonists and functional antagonist partially blocked, and can be OFQ strengthened, suggesting that EA may analgesic effect through the spinal cord OFQ system. Spinal cord OFQ and its receptor in the rat neuropathic pain and electro-acupuncture treatment of neuropathic pain plasticity CCI neuropathic pain in rats after 7 days and given electro-acupuncture, spinal cord dorsal horn OFQ and its receptor expression changes. The experiment was divided into three groups: normal group (Normal), neuralgia group (NP), electro-acupuncture group (EA). 2.1 the spinal dorsal angle ppOFQ mRNA in rat neuralgia and EA to changes in three groups of rats 7 days after perfusion, the rat spinal cord lumbar enlargement segment frozen
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